Factors involved in the cisplatin resistance of KCP‑4 human epidermoid carcinoma cells

  • Authors:
    • Shigeru Oiso
    • Yui Takayama
    • Rie Nakazaki
    • Naoko Matsunaga
    • Chie Motooka
    • Asuka Yamamura
    • Ryuji Ikeda
    • Kazuo Nakamura
    • Yasuo Takeda
    • Hiroko Kariyazono
  • View Affiliations

  • Published online on: December 5, 2013     https://doi.org/10.3892/or.2013.2896
  • Pages: 719-726
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Abstract

KCP-4 is a cisplatin-resistant cell line established from human epidermoid carcinoma KB-3-1 cells. Although our previous study revealed that one of the mechanisms for cisplatin resistance in KCP-4 cells is the activation of NF-κB, its high resistance is considered to be induced by multiple mechanisms. In the present study, we explored other factors involved in the development of cisplatin resistance in KCP-4 cells. Since it has been reported that an unknown efflux pump exports cisplatin from KCP-4 cells in an ATP-dependent manner, we examined 48 types of ATP-binding cassette proteins as candidate cisplatin efflux transporters. The mRNA expression levels of ABCA1, ABCA3, ABCA7 and ABCB10 in KCP-4 cells were higher when compared to those in KB-3-1 cells. These expression levels in cisplatin-sensitive revertant KCP-4 cells (KCP-4R cells), were reduced in parallel with the sensitivity of these cells to cisplatin and their intracellular accumulation of cisplatin. Next, we investigated the occurrence of mutations in p53 in KCP-4 cells. We found a heterozygous missense mutation at codon 72 (p.Pro72Arg) in p53 of both KCP-4 and KB-3-1 cells, but the protein expression level of p53 in KCP-4 cells was higher when compared to that in KB-3-1. These results suggest that ABCA1, ABCA3, ABCA7 and ABCB10 are candidate genes for the cisplatin efflux transporter that is involved in the cisplatin resistance of KCP-4 cells, and that the mutation at codon 72 of p53 may contribute to the development of cisplatin resistance.
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2014-February
Volume 31 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Oiso S, Takayama Y, Nakazaki R, Matsunaga N, Motooka C, Yamamura A, Ikeda R, Nakamura K, Takeda Y, Kariyazono H, Kariyazono H, et al: Factors involved in the cisplatin resistance of KCP‑4 human epidermoid carcinoma cells. Oncol Rep 31: 719-726, 2014
APA
Oiso, S., Takayama, Y., Nakazaki, R., Matsunaga, N., Motooka, C., Yamamura, A. ... Kariyazono, H. (2014). Factors involved in the cisplatin resistance of KCP‑4 human epidermoid carcinoma cells. Oncology Reports, 31, 719-726. https://doi.org/10.3892/or.2013.2896
MLA
Oiso, S., Takayama, Y., Nakazaki, R., Matsunaga, N., Motooka, C., Yamamura, A., Ikeda, R., Nakamura, K., Takeda, Y., Kariyazono, H."Factors involved in the cisplatin resistance of KCP‑4 human epidermoid carcinoma cells". Oncology Reports 31.2 (2014): 719-726.
Chicago
Oiso, S., Takayama, Y., Nakazaki, R., Matsunaga, N., Motooka, C., Yamamura, A., Ikeda, R., Nakamura, K., Takeda, Y., Kariyazono, H."Factors involved in the cisplatin resistance of KCP‑4 human epidermoid carcinoma cells". Oncology Reports 31, no. 2 (2014): 719-726. https://doi.org/10.3892/or.2013.2896