miR-200b suppresses cell proliferation, migration and enhances chemosensitivity in prostate cancer by regulating Bmi-1

  • Authors:
    • Junjie Yu
    • Youyi Lu
    • Di Cui
    • Enhui Li
    • Yipin Zhu
    • Yuyang Zhao
    • Fujun Zhao
    • Shujie Xia
  • View Affiliations

  • Published online on: December 5, 2013     https://doi.org/10.3892/or.2013.2897
  • Pages: 910-918
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

microRNAs (miRNAs) are a class of small non-coding RNAs that can post-transcriptionally regulate gene expression and play critical roles in many important biological processes. The role of miRNAs in prostate cancer (PCa) development and pathogenesis remains largely unknown. In the present study, we showed that miR-200b was downregulated in clinical prostatic tumors when compared to normal prostate tissue and in advanced PCa cell lines when compared to normal epithelial prostatic cells. Enforced miR-200b expression suppressed PCa cell proliferation and migration and enhanced chemosensitivity to docetaxel by targeting B-cell-specific Moloney murine leukemia virus insertion site 1 (Bmi-1). Bmi-1 was detected at higher levels in PCa, and knockdown of Bmi-1 showed similar effects as miR-200b overexpression in PCa cells. Moreover, we confirmed that these effects were correlated with increased levels of E-cadherin and P16 and a reduction in vimentin expression and expression of stem cell markers (CD44 and OCT4). These findings suggest that miR-200b plays vital roles as a tumor-suppressor by targeting Bmi-1 and may be a promising therapeutic target for PCa treatment.
View Figures
View References

Related Articles

Journal Cover

February 2014
Volume 31 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Yu, J., Lu, Y., Cui, D., Li, E., Zhu, Y., Zhao, Y. ... Xia, S. (2014). miR-200b suppresses cell proliferation, migration and enhances chemosensitivity in prostate cancer by regulating Bmi-1. Oncology Reports, 31, 910-918. https://doi.org/10.3892/or.2013.2897
MLA
Yu, J., Lu, Y., Cui, D., Li, E., Zhu, Y., Zhao, Y., Zhao, F., Xia, S."miR-200b suppresses cell proliferation, migration and enhances chemosensitivity in prostate cancer by regulating Bmi-1". Oncology Reports 31.2 (2014): 910-918.
Chicago
Yu, J., Lu, Y., Cui, D., Li, E., Zhu, Y., Zhao, Y., Zhao, F., Xia, S."miR-200b suppresses cell proliferation, migration and enhances chemosensitivity in prostate cancer by regulating Bmi-1". Oncology Reports 31, no. 2 (2014): 910-918. https://doi.org/10.3892/or.2013.2897