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Article

A preliminary study on the construction of double suicide gene delivery vectors by mesenchymal stem cells and the in vitro inhibitory effects on SKOV3 cells

  • Authors:
    • Jingyan Jiang
    • Dee Wei
    • Li Sun
    • Yuxia Wang
    • Xihai Wu
    • Ying Li
    • Zhenghui Fang
    • Hui Shang
    • Zengtao Wei
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250014, P.R. China, Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan, Shandong 250117, P.R. China, Department of Stomatology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250014, P.R. China
  • Pages: 781-787
    |
    Published online on: December 5, 2013
       https://doi.org/10.3892/or.2013.2898
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Abstract

The aim of the present study was to investigate the efficacy of using human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) as gene delivery vectors in the treatment of ovarian cancer. Lentivectors overexpressing cytosine deaminase (CD) and herpes simplex virus thymidine kinase (HSV-tk) (pGC-FU-CD-TK) were constructed, and confirmed by enzyme digestion, DNA sequence and western blotting. Quantitative PCR (PCR) was used to verify the overexpression of the fusion gene (CD and HSV-tk). SKOV3 cells were co-cultured with MSCs/tk+CD+ at a 1:1 ratio, and were then treated with the prodrugs (GCV) and/or 5-fluorocytosine (5-FC) at different concentrations, and the cytotoxic effects were evaluated using MTT assay and flow cytometry. DNA sequencing demonstrated that the sequence of HSV-tk and CD genes were consistent with the objective sequence and western blotting verified that the constructed lentivector could produce the HSV-tk/CD gene. The packed titer was 2.00e+8 TU/ml. The pGC-FU-CD-TK could be stably transferred to hUCB‑MSCs, and the infection efficiency was almost 80%. RT-PCR demonstrated that the expression levels of the HSV-tk/CD fusion gene in MSCs/tk+CD+ group was 75 times that in the negative control (P<0.05). Compared with GCV or 5-FC alone, the growth inhibition rate (GIR) was significantly higher in the combined treatment (F=85.35, P<0.05). The reconstructed MSCs/tk+CD+ vectors were capable of slowing down the growth of human SKOV3 cells in the presence of prodrugs in vitro. The use of combination chemotherapy exhibited a more significant inhibitory effect than using a single prodrug.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang J, Wei D, Sun L, Wang Y, Wu X, Li Y, Fang Z, Shang H and Wei Z: A preliminary study on the construction of double suicide gene delivery vectors by mesenchymal stem cells and the in vitro inhibitory effects on SKOV3 cells. Oncol Rep 31: 781-787, 2014.
APA
Jiang, J., Wei, D., Sun, L., Wang, Y., Wu, X., Li, Y. ... Wei, Z. (2014). A preliminary study on the construction of double suicide gene delivery vectors by mesenchymal stem cells and the in vitro inhibitory effects on SKOV3 cells. Oncology Reports, 31, 781-787. https://doi.org/10.3892/or.2013.2898
MLA
Jiang, J., Wei, D., Sun, L., Wang, Y., Wu, X., Li, Y., Fang, Z., Shang, H., Wei, Z."A preliminary study on the construction of double suicide gene delivery vectors by mesenchymal stem cells and the in vitro inhibitory effects on SKOV3 cells". Oncology Reports 31.2 (2014): 781-787.
Chicago
Jiang, J., Wei, D., Sun, L., Wang, Y., Wu, X., Li, Y., Fang, Z., Shang, H., Wei, Z."A preliminary study on the construction of double suicide gene delivery vectors by mesenchymal stem cells and the in vitro inhibitory effects on SKOV3 cells". Oncology Reports 31, no. 2 (2014): 781-787. https://doi.org/10.3892/or.2013.2898
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang J, Wei D, Sun L, Wang Y, Wu X, Li Y, Fang Z, Shang H and Wei Z: A preliminary study on the construction of double suicide gene delivery vectors by mesenchymal stem cells and the in vitro inhibitory effects on SKOV3 cells. Oncol Rep 31: 781-787, 2014.
APA
Jiang, J., Wei, D., Sun, L., Wang, Y., Wu, X., Li, Y. ... Wei, Z. (2014). A preliminary study on the construction of double suicide gene delivery vectors by mesenchymal stem cells and the in vitro inhibitory effects on SKOV3 cells. Oncology Reports, 31, 781-787. https://doi.org/10.3892/or.2013.2898
MLA
Jiang, J., Wei, D., Sun, L., Wang, Y., Wu, X., Li, Y., Fang, Z., Shang, H., Wei, Z."A preliminary study on the construction of double suicide gene delivery vectors by mesenchymal stem cells and the in vitro inhibitory effects on SKOV3 cells". Oncology Reports 31.2 (2014): 781-787.
Chicago
Jiang, J., Wei, D., Sun, L., Wang, Y., Wu, X., Li, Y., Fang, Z., Shang, H., Wei, Z."A preliminary study on the construction of double suicide gene delivery vectors by mesenchymal stem cells and the in vitro inhibitory effects on SKOV3 cells". Oncology Reports 31, no. 2 (2014): 781-787. https://doi.org/10.3892/or.2013.2898
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