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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Article

Silencing Dicer expression enhances cellular proliferative and invasive capacities in human tongue squamous cell carcinoma

  • Authors:
    • Shuguang Zeng
    • Jing Yang
    • Jianjiang Zhao
    • Qicai Liu
    • Mingdeng Rong
    • Zehong Guo
    • Wenfeng Gao
  • View Affiliations / Copyright

    Affiliations: Stomatological Hospital of Guangdong Province Affiliated to Southern Medical University, Guangzhou, Guangdong 510280, P.R. China, Department of Molecular Biology, Guangzhou Medical College, Guangzhou, Guangdong 510120, P.R. China
  • Pages: 867-873
    |
    Published online on: December 6, 2013
       https://doi.org/10.3892/or.2013.2903
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Abstract

microRNAs (miRNAs) are aberrantly expressed in cancer. An enzyme essential for miRNA processing is Dicer, whose expression is deregulated in diverse types of cancer and correlates with tumor progression. However, whether the regulation of Dicer expression affects tongue squamous cell carcinoma is unknown. In the present study, we investigated how silencing the expression of Dicer alters cell proliferation, cell cycle patterns, and cell migration and invasion in the Tca-8113 tongue squamous cell carcinoma cell line. Dicer expression levels were determined using quantitative PCR and western blot analysis in normal oral gingival epithelial cells and in two tongue squamous cell carcinoma lines, Tca-8113 and UM-1. Tca-8113 cells were transfected with Dicer siRNA or a negative control siRNA. Cell proliferation was determined using the MTT assay and the cell cycle was examined using flow cytometry. Cell migration and invasion changes were evaluated using wound-healing, adherence and Transwell assays. Dicer was expressed at lower levels in the tongue squamous cell carcinoma cell lines Tca-8113 and UM-1 compared to normal gingival epithelial cells, and less Dicer was expressed in UM-1 cells compared to Tca-8113 cells. Notably, Tca-8113 cells transfected with Dicer siRNA had significantly higher proliferative and invasive abilities than cells transfected with the negative control siRNA or non-transfected cells. Silencing Dicer may promote the progression of tongue squamous cell carcinoma. Dicer could serve a promising biomarker and a potential therapeutic target for tongue squamous cell carcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Zeng S, Yang J, Zhao J, Liu Q, Rong M, Guo Z and Gao W: Silencing Dicer expression enhances cellular proliferative and invasive capacities in human tongue squamous cell carcinoma. Oncol Rep 31: 867-873, 2014.
APA
Zeng, S., Yang, J., Zhao, J., Liu, Q., Rong, M., Guo, Z., & Gao, W. (2014). Silencing Dicer expression enhances cellular proliferative and invasive capacities in human tongue squamous cell carcinoma. Oncology Reports, 31, 867-873. https://doi.org/10.3892/or.2013.2903
MLA
Zeng, S., Yang, J., Zhao, J., Liu, Q., Rong, M., Guo, Z., Gao, W."Silencing Dicer expression enhances cellular proliferative and invasive capacities in human tongue squamous cell carcinoma". Oncology Reports 31.2 (2014): 867-873.
Chicago
Zeng, S., Yang, J., Zhao, J., Liu, Q., Rong, M., Guo, Z., Gao, W."Silencing Dicer expression enhances cellular proliferative and invasive capacities in human tongue squamous cell carcinoma". Oncology Reports 31, no. 2 (2014): 867-873. https://doi.org/10.3892/or.2013.2903
Copy and paste a formatted citation
x
Spandidos Publications style
Zeng S, Yang J, Zhao J, Liu Q, Rong M, Guo Z and Gao W: Silencing Dicer expression enhances cellular proliferative and invasive capacities in human tongue squamous cell carcinoma. Oncol Rep 31: 867-873, 2014.
APA
Zeng, S., Yang, J., Zhao, J., Liu, Q., Rong, M., Guo, Z., & Gao, W. (2014). Silencing Dicer expression enhances cellular proliferative and invasive capacities in human tongue squamous cell carcinoma. Oncology Reports, 31, 867-873. https://doi.org/10.3892/or.2013.2903
MLA
Zeng, S., Yang, J., Zhao, J., Liu, Q., Rong, M., Guo, Z., Gao, W."Silencing Dicer expression enhances cellular proliferative and invasive capacities in human tongue squamous cell carcinoma". Oncology Reports 31.2 (2014): 867-873.
Chicago
Zeng, S., Yang, J., Zhao, J., Liu, Q., Rong, M., Guo, Z., Gao, W."Silencing Dicer expression enhances cellular proliferative and invasive capacities in human tongue squamous cell carcinoma". Oncology Reports 31, no. 2 (2014): 867-873. https://doi.org/10.3892/or.2013.2903
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