Cells susceptible to epithelial-mesenchymal transition are enriched in stem-like side population cells from prostate cancer

  • Authors:
    • Yong Luo
    • Xinhao Cui
    • Jiahui Zhao
    • Yili Han
    • Mingchuan Li
    • Yunhua Lin
    • Yongguang Jiang
    • Ling Lan
  • View Affiliations

  • Published online on: December 6, 2013     https://doi.org/10.3892/or.2013.2905
  • Pages: 874-884
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Accumulating evidence suggests that epithelial-mesenchymal transition (EMT) acts as an important factor for the promotion of tumor progression. Strategies for suppressing EMT remain the subject of ongoing research. In the present study, fluorescence-activated cell sorting (FACS) was used to isolate side population (SP) cells from human prostate cancer (PCa) cell lines and xenograft tissues. After identifying their molecular and functional stem-like characteristics, stem-like SP cells from a cell line and from xenograft tissue were transfected with hypoxia inducible factor-1α (HIF-1α). The potential of the prostate stem-like SP cells to undergo EMT was compared with that in their bulk counterparts after HIF-1α introduction. Stem-like SP cells acquired more complete EMT molecular features and exhibited stronger aggressive capability than the homologous bulk population cells both in vitro (proliferation and invasion) and in vivo (tumorigenesis and metastasis formation). We, therefore, concluded that EMT is closely associated with tumor heterogeneity, and that PCa cells susceptible to EMT are enriched in stem-like SP cells. These findings disclose a new approach, targeting the cellular basis of the EMT process that may help to identify effective and accurate methods for suppressing tumor growth and preventing distant dissemination.
View Figures
View References

Related Articles

Journal Cover

February 2014
Volume 31 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Luo, Y., Cui, X., Zhao, J., Han, Y., Li, M., Lin, Y. ... Lan, L. (2014). Cells susceptible to epithelial-mesenchymal transition are enriched in stem-like side population cells from prostate cancer. Oncology Reports, 31, 874-884. https://doi.org/10.3892/or.2013.2905
MLA
Luo, Y., Cui, X., Zhao, J., Han, Y., Li, M., Lin, Y., Jiang, Y., Lan, L."Cells susceptible to epithelial-mesenchymal transition are enriched in stem-like side population cells from prostate cancer". Oncology Reports 31.2 (2014): 874-884.
Chicago
Luo, Y., Cui, X., Zhao, J., Han, Y., Li, M., Lin, Y., Jiang, Y., Lan, L."Cells susceptible to epithelial-mesenchymal transition are enriched in stem-like side population cells from prostate cancer". Oncology Reports 31, no. 2 (2014): 874-884. https://doi.org/10.3892/or.2013.2905