Open Access

Capsaicin induces apoptosis in PC12 cells through ER stress

  • Authors:
    • Olga Krizanova
    • Iveta Steliarova
    • Lucia Csaderova
    • Michal Pastorek
    • Sona Hudecova
  • View Affiliations

  • Published online on: December 13, 2013     https://doi.org/10.3892/or.2013.2921
  • Pages: 581-588
  • Copyright: © Krizanova et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Capsaicin, the pungent agent in chili peppers, has been shown to act as a tumor-suppressor in cancer. In our previous study, capsaicin was shown to induce apoptosis in the rat pheochromocytoma cell line (PC12 cells). Thus, the aim of the present study was to determine the potential mechanism by which capsaicin induces apoptosis. We treated PC12 cells with 50, 100 and 500 µM capsaicin and measured the reticular calcium content and expression of the reticular calcium transport systems. These results were correlated with endoplasmic reticulum (ER) stress markers CHOP, ATF4 and X-box binding protein 1 (XBP1), as well as with apoptosis induction. We observed that capsaicin decreased reticular calcium in a concentration-dependent manner. Simultaneously, expression levels of the sarco/endoplasmic reticulum pump and ryanodin receptor of type 2 were modified. These changes were accompanied by increased ER stress, as documented by increased stress markers. Thus, from these results we propose that in PC12 cells capsaicin induces apoptosis through increased ER stress.
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2014-February
Volume 31 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Krizanova O, Steliarova I, Csaderova L, Pastorek M and Hudecova S: Capsaicin induces apoptosis in PC12 cells through ER stress. Oncol Rep 31: 581-588, 2014.
APA
Krizanova, O., Steliarova, I., Csaderova, L., Pastorek, M., & Hudecova, S. (2014). Capsaicin induces apoptosis in PC12 cells through ER stress. Oncology Reports, 31, 581-588. https://doi.org/10.3892/or.2013.2921
MLA
Krizanova, O., Steliarova, I., Csaderova, L., Pastorek, M., Hudecova, S."Capsaicin induces apoptosis in PC12 cells through ER stress". Oncology Reports 31.2 (2014): 581-588.
Chicago
Krizanova, O., Steliarova, I., Csaderova, L., Pastorek, M., Hudecova, S."Capsaicin induces apoptosis in PC12 cells through ER stress". Oncology Reports 31, no. 2 (2014): 581-588. https://doi.org/10.3892/or.2013.2921