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2014-March Volume 31 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Article

The heme precursor 5-aminolevulinic acid disrupts the Warburg effect in tumor cells and induces caspase-dependent apoptosis

  • Authors:
    • Yuta Sugiyama
    • Yuichiro Hagiya
    • Motowo Nakajima
    • Masahiro Ishizuka
    • Tohru Tanaka
    • Shun-Ichiro Ogura
  • View Affiliations / Copyright

    Affiliations: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa 226‑8501, Japan, SBI Pharmaceuticals Co., Ltd., Tokyo 106-6020, Japan
  • Pages: 1282-1286
    |
    Published online on: December 23, 2013
       https://doi.org/10.3892/or.2013.2945
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Abstract

Our previous study demonstrated that 5-aminolevulinic acid (ALA) administered to mice stimulates oxidative phosphorylation by upregulation of the mitochondrial respiratory chain complex IV enzyme cytochrome c oxidase (COX). The present study investigated whether ALA disrupts the Warburg effect, which represents a shift in ATP generation from oxidative phosphorylation to glycolysis, protecting tumor cells against oxidative stress-mediated apoptosis. The human lung carcinoma cell line A549 exposed to ALA exhibited enhanced oxidative phosphorylation, which was indicated by an increase in COX protein expression and oxygen consumption. Furthermore, ALA suppressed glycolysis-mediated acidosis. This normalization of the ATP metabolic pathways significantly increased the generation of superoxide anion radical (O2•-) and the functional expression of active caspase-3, leading to caspase-dependent apoptosis. These data demonstrate that ALA inhibits the Warburg effect and induces cancer cell death. Use of this endogenous compound might constitute a novel approach to cancer therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Sugiyama Y, Hagiya Y, Nakajima M, Ishizuka M, Tanaka T and Ogura S: The heme precursor 5-aminolevulinic acid disrupts the Warburg effect in tumor cells and induces caspase-dependent apoptosis. Oncol Rep 31: 1282-1286, 2014.
APA
Sugiyama, Y., Hagiya, Y., Nakajima, M., Ishizuka, M., Tanaka, T., & Ogura, S. (2014). The heme precursor 5-aminolevulinic acid disrupts the Warburg effect in tumor cells and induces caspase-dependent apoptosis. Oncology Reports, 31, 1282-1286. https://doi.org/10.3892/or.2013.2945
MLA
Sugiyama, Y., Hagiya, Y., Nakajima, M., Ishizuka, M., Tanaka, T., Ogura, S."The heme precursor 5-aminolevulinic acid disrupts the Warburg effect in tumor cells and induces caspase-dependent apoptosis". Oncology Reports 31.3 (2014): 1282-1286.
Chicago
Sugiyama, Y., Hagiya, Y., Nakajima, M., Ishizuka, M., Tanaka, T., Ogura, S."The heme precursor 5-aminolevulinic acid disrupts the Warburg effect in tumor cells and induces caspase-dependent apoptosis". Oncology Reports 31, no. 3 (2014): 1282-1286. https://doi.org/10.3892/or.2013.2945
Copy and paste a formatted citation
x
Spandidos Publications style
Sugiyama Y, Hagiya Y, Nakajima M, Ishizuka M, Tanaka T and Ogura S: The heme precursor 5-aminolevulinic acid disrupts the Warburg effect in tumor cells and induces caspase-dependent apoptosis. Oncol Rep 31: 1282-1286, 2014.
APA
Sugiyama, Y., Hagiya, Y., Nakajima, M., Ishizuka, M., Tanaka, T., & Ogura, S. (2014). The heme precursor 5-aminolevulinic acid disrupts the Warburg effect in tumor cells and induces caspase-dependent apoptosis. Oncology Reports, 31, 1282-1286. https://doi.org/10.3892/or.2013.2945
MLA
Sugiyama, Y., Hagiya, Y., Nakajima, M., Ishizuka, M., Tanaka, T., Ogura, S."The heme precursor 5-aminolevulinic acid disrupts the Warburg effect in tumor cells and induces caspase-dependent apoptosis". Oncology Reports 31.3 (2014): 1282-1286.
Chicago
Sugiyama, Y., Hagiya, Y., Nakajima, M., Ishizuka, M., Tanaka, T., Ogura, S."The heme precursor 5-aminolevulinic acid disrupts the Warburg effect in tumor cells and induces caspase-dependent apoptosis". Oncology Reports 31, no. 3 (2014): 1282-1286. https://doi.org/10.3892/or.2013.2945
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