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Article

The role of plasma cell-free DNA detection in predicting preoperative chemoradiotherapy response in rectal cancer patients

  • Authors:
    • W. Sun
    • Y. Sun
    • M. Zhu
    • Z. Wang
    • H. Zhang
    • Y. Xin
    • G. Jiang
    • X. Guo
    • Z. Zhang
    • Y. Liu
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai 200032, P.R. China, Cancer Research Institute of Fudan University Shanghai Cancer Center, Shanghai 200032, P.R. China
  • Pages: 1466-1472
    |
    Published online on: December 30, 2013
       https://doi.org/10.3892/or.2013.2949
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Abstract

In the present study, we studied the relationship between plasma cell-free DNA and the effect of preoperative chemoradiotherapy in patients with rectal cancer. The concentration, KRAS mutation and O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation status of cell-free DNA were measured by using polymerase chain reaction (PCR) analyses. The response to chemoradiotherapy was assessed using tumor regression grading (TRG) scores. The cell-free DNA concentrations in patients with rectal cancer (n=34) were significantly higher compared to healthy controls (n=10). The 400-base pair (bp) DNA concentration, 400-/100-bp DNA ratio decreased significantly after chemoradiotherapy in the good response group. The incidence of KRAS mutation decreased significantly after chemoradiotherapy in both good and poor response groups. Higher MGMT promoter methylation status at baseline DNA was associated with a better tumor response. Therefore, cell-free DNA detection may be useful in evaluating the effect of preoperative chemoradiotherapy in patients with rectal cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Sun W, Sun Y, Zhu M, Wang Z, Zhang H, Xin Y, Jiang G, Guo X, Zhang Z, Liu Y, Liu Y, et al: The role of plasma cell-free DNA detection in predicting preoperative chemoradiotherapy response in rectal cancer patients. Oncol Rep 31: 1466-1472, 2014.
APA
Sun, W., Sun, Y., Zhu, M., Wang, Z., Zhang, H., Xin, Y. ... Liu, Y. (2014). The role of plasma cell-free DNA detection in predicting preoperative chemoradiotherapy response in rectal cancer patients. Oncology Reports, 31, 1466-1472. https://doi.org/10.3892/or.2013.2949
MLA
Sun, W., Sun, Y., Zhu, M., Wang, Z., Zhang, H., Xin, Y., Jiang, G., Guo, X., Zhang, Z., Liu, Y."The role of plasma cell-free DNA detection in predicting preoperative chemoradiotherapy response in rectal cancer patients". Oncology Reports 31.3 (2014): 1466-1472.
Chicago
Sun, W., Sun, Y., Zhu, M., Wang, Z., Zhang, H., Xin, Y., Jiang, G., Guo, X., Zhang, Z., Liu, Y."The role of plasma cell-free DNA detection in predicting preoperative chemoradiotherapy response in rectal cancer patients". Oncology Reports 31, no. 3 (2014): 1466-1472. https://doi.org/10.3892/or.2013.2949
Copy and paste a formatted citation
x
Spandidos Publications style
Sun W, Sun Y, Zhu M, Wang Z, Zhang H, Xin Y, Jiang G, Guo X, Zhang Z, Liu Y, Liu Y, et al: The role of plasma cell-free DNA detection in predicting preoperative chemoradiotherapy response in rectal cancer patients. Oncol Rep 31: 1466-1472, 2014.
APA
Sun, W., Sun, Y., Zhu, M., Wang, Z., Zhang, H., Xin, Y. ... Liu, Y. (2014). The role of plasma cell-free DNA detection in predicting preoperative chemoradiotherapy response in rectal cancer patients. Oncology Reports, 31, 1466-1472. https://doi.org/10.3892/or.2013.2949
MLA
Sun, W., Sun, Y., Zhu, M., Wang, Z., Zhang, H., Xin, Y., Jiang, G., Guo, X., Zhang, Z., Liu, Y."The role of plasma cell-free DNA detection in predicting preoperative chemoradiotherapy response in rectal cancer patients". Oncology Reports 31.3 (2014): 1466-1472.
Chicago
Sun, W., Sun, Y., Zhu, M., Wang, Z., Zhang, H., Xin, Y., Jiang, G., Guo, X., Zhang, Z., Liu, Y."The role of plasma cell-free DNA detection in predicting preoperative chemoradiotherapy response in rectal cancer patients". Oncology Reports 31, no. 3 (2014): 1466-1472. https://doi.org/10.3892/or.2013.2949
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