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Article

MicroRNA-503 acts as a tumor suppressor in glioblastoma for multiple antitumor effects by targeting IGF-1R

  • Authors:
    • Yingying Zhang
    • Xiong Chen
    • Haiwei Lian
    • Jianmiao Liu
    • Beiyan Zhou
    • Song Han
    • Biwen Peng
    • Jun Yin
    • Wanhong Liu
    • Xiaohua He
  • View Affiliations / Copyright

    Affiliations: Hubei Province Key Laboratory of Allergy and Immunology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei 430071, P.R. China, Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, P.R. China, Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA
  • Pages: 1445-1452
    |
    Published online on: December 30, 2013
       https://doi.org/10.3892/or.2013.2951
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Abstract

microRNA (miRNA) dysregulation is associated with various types of human cancer by regulating cancer cell survival, proliferation and invasion. Aberrant expression of microRNA-503 (miR-503) has been reported in several cancer profiles. However, potential linkage of miR-503 levels and the underlying regulatory mechanisms in human glioblastoma multiforme (GBM) remain unclear. In the present study, we showed for the first time that the expression of miR-503 was significantly reduced in GBM tissues and cell lines (U251 and U87MG) relative to normal brain tissues. Furthermore, our results demonstrated that overexpression of miR-503 in GBM cell lines not only suppressed cell proliferation through inducing G0/G1 cell cycle arrest and apoptosis, but also inhibited cancer cell migration and tumor invasion. In addition, we identified insulin-like growth factor-1 (IGF‑1R) receptor mRNA is a bona fide target of miR-503 by computational analysis followed by luciferase reporter assays. Of note, upregulation of miR-503 in GBM cells suppressed endogenous IGF-1R protein expression. Further mechanistic analysis revealed that forced expression of miR-503 inhibited AKT activation, suggesting the tumor suppressive effect of miR-503 in GBM cells is partially mediated by phosphatidylinositol 3-kinase/AKT signaling. Taken together, the results of the present study demonstrated that miR-503 is a tumor suppressor for GBM and a favorable factor against glioma progression through targeting IGF-1R, thus providing a new evidence-supported prognostic marker for GBM diagnosis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Chen X, Lian H, Liu J, Zhou B, Han S, Peng B, Yin J, Liu W, He X, He X, et al: MicroRNA-503 acts as a tumor suppressor in glioblastoma for multiple antitumor effects by targeting IGF-1R. Oncol Rep 31: 1445-1452, 2014.
APA
Zhang, Y., Chen, X., Lian, H., Liu, J., Zhou, B., Han, S. ... He, X. (2014). MicroRNA-503 acts as a tumor suppressor in glioblastoma for multiple antitumor effects by targeting IGF-1R. Oncology Reports, 31, 1445-1452. https://doi.org/10.3892/or.2013.2951
MLA
Zhang, Y., Chen, X., Lian, H., Liu, J., Zhou, B., Han, S., Peng, B., Yin, J., Liu, W., He, X."MicroRNA-503 acts as a tumor suppressor in glioblastoma for multiple antitumor effects by targeting IGF-1R". Oncology Reports 31.3 (2014): 1445-1452.
Chicago
Zhang, Y., Chen, X., Lian, H., Liu, J., Zhou, B., Han, S., Peng, B., Yin, J., Liu, W., He, X."MicroRNA-503 acts as a tumor suppressor in glioblastoma for multiple antitumor effects by targeting IGF-1R". Oncology Reports 31, no. 3 (2014): 1445-1452. https://doi.org/10.3892/or.2013.2951
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Chen X, Lian H, Liu J, Zhou B, Han S, Peng B, Yin J, Liu W, He X, He X, et al: MicroRNA-503 acts as a tumor suppressor in glioblastoma for multiple antitumor effects by targeting IGF-1R. Oncol Rep 31: 1445-1452, 2014.
APA
Zhang, Y., Chen, X., Lian, H., Liu, J., Zhou, B., Han, S. ... He, X. (2014). MicroRNA-503 acts as a tumor suppressor in glioblastoma for multiple antitumor effects by targeting IGF-1R. Oncology Reports, 31, 1445-1452. https://doi.org/10.3892/or.2013.2951
MLA
Zhang, Y., Chen, X., Lian, H., Liu, J., Zhou, B., Han, S., Peng, B., Yin, J., Liu, W., He, X."MicroRNA-503 acts as a tumor suppressor in glioblastoma for multiple antitumor effects by targeting IGF-1R". Oncology Reports 31.3 (2014): 1445-1452.
Chicago
Zhang, Y., Chen, X., Lian, H., Liu, J., Zhou, B., Han, S., Peng, B., Yin, J., Liu, W., He, X."MicroRNA-503 acts as a tumor suppressor in glioblastoma for multiple antitumor effects by targeting IGF-1R". Oncology Reports 31, no. 3 (2014): 1445-1452. https://doi.org/10.3892/or.2013.2951
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