Novel drug discovery system for cancer stem cells in human squamous cell carcinoma of the esophagus

Kano,Yoshihiro; Konno,Masamitsu; Kawamoto,Koichi; Tamari,Keisuke; Hayashi,Kazuhiko; Fukusumi,Takahito; Satoh,Taroh; Tanaka,Shinji; Ogawa,Kazuhiko; Mori,Masaki; Doki,Yuichiro; Ishii,Hideshi

doi:10.3892/or.2013.2952

2014-March Volume 31 Issue 3

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2014-March Volume 31 Issue 3

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Article

Novel drug discovery system for cancer stem cells in human squamous cell carcinoma of the esophagus

Authors:
- Yoshihiro Kano
- Masamitsu Konno
- Koichi Kawamoto
- Keisuke Tamari
- Kazuhiko Hayashi
- Takahito Fukusumi
- Taroh Satoh
- Shinji Tanaka
- Kazuhiko Ogawa
- Masaki Mori
- Yuichiro Doki
- Hideshi Ishii
View Affiliations / Copyright

Affiliations: Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan, Department of Frontier Science for Cancer and Chemotherapy, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan, Department of Radiation Oncology, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan, Department of Otorhinolaryngology-Head and Neck Surgery, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan, Department of Hepato-Biliary-Pancreatic Surgery, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo 113-0034, Japan
Pages: 1133-1138
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Published online on: December 30, 2013

https://doi.org/10.3892/or.2013.2952
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Abstract

Cancer stem cells (CSCs) have been identified in several tumor tissues. Since CSCs are resistant to cancer therapies, including chemotherapy and radiation therapy, and can even remain after therapies, tumor tissue often regrows and relapses. Thus, identification of CSCs and treatment targeting CSCs are required to treat tumor tissues. Reportedly, a fluorescent vector consisting of fluorescein ZsGreen fused to the carboxyl-terminal region of ornithine decarboxylase (cODC) was used to detect CSCs or therapy-resistant cancer cells in tumor tissues of the brain, pancreas and liver. Cells transfected with the fluorescent vector can express a fluorescein fused to cODC and become fluorescent only when the fusion protein is accumulated. In the present study, CSCs or therapy-resistant cancer cells were identified with the fluorescent vector in esophageal squamous cell carcinoma. The use of this fluorescent vector in drug screening enabled the detection of three drugs, AKT inhibitor XI, ERK inhibitor II and JAK inhibitor I, which target malignant CSCs.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Novel drug discovery system for cancer stem cells in human squamous cell carcinoma of the esophagus

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