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Article

Upregulation of miR-194 contributes to tumor growth and progression in pancreatic ductal adenocarcinoma

  • Authors:
    • Jing Zhang
    • Chen-Yan Zhao
    • Shu-Hui Zhang
    • Dang-Hui Yu
    • Ying Chen
    • Qing-Hua Liu
    • Min Shi
    • Can-Rong Ni
    • Ming-Hua Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China, Department of Pathology, Yueyang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, P.R. China
  • Pages: 1157-1164
    |
    Published online on: December 31, 2013
       https://doi.org/10.3892/or.2013.2960
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Abstract

Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal types of human cancer worldwide. In the present study, we investigated the diagnostic and biological significance of microRNA-194 (miR-194) in PDAC. miRNA expression profiling of human PDACs and adjacent normal pancreatic tissues identified a total of 16 genes including miR-194 with >1.15-fold expression changes (8 overexpressed and 8 underexpressed). Quantitative real-time polymerase chain reaction (PCR) revealed elevation of serum miR-194 levels were significantly greater in PDAC patients than in duodenal adenocarcinoma patients and healthy controls. Receiver operating characteristic analysis demonstrated that serum miR-194 had a sensitivity of 54.3% and a specificity of 57.5% for discriminating PDAC patients from healthy controls. Combined analysis of the 3 groups yielded a sensitivity of 84.0 and a specificity of 75.0% for the combined detection of miR-192 and miR-194 in the diagnosis of PDAC. Ectopic expression of miR-194 in PANC-1 pancreatic cancer cells enhanced cell proliferation, migration and colony formation, which was coupled with decreased expression of the tumor suppressor DACH1. miR-194 overexpression increased tumor growth and local invasion and suppressed the expression of DACH1 in an orthotopic pancreatic cancer mouse model. In conclusion, upregulation of miR-194 contributes to tumor growth and progression in PDAC, possibly through suppression of DACH1. However, serum miR-194 has a low capacity for detection of PDAC. Combined detection of serum miR-192 and miR-194 levels may serve as a sensitive diagnostic biomarker for PDAC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Zhao C, Zhang S, Yu D, Chen Y, Liu Q, Shi M, Ni C and Zhu M: Upregulation of miR-194 contributes to tumor growth and progression in pancreatic ductal adenocarcinoma. Oncol Rep 31: 1157-1164, 2014.
APA
Zhang, J., Zhao, C., Zhang, S., Yu, D., Chen, Y., Liu, Q. ... Zhu, M. (2014). Upregulation of miR-194 contributes to tumor growth and progression in pancreatic ductal adenocarcinoma. Oncology Reports, 31, 1157-1164. https://doi.org/10.3892/or.2013.2960
MLA
Zhang, J., Zhao, C., Zhang, S., Yu, D., Chen, Y., Liu, Q., Shi, M., Ni, C., Zhu, M."Upregulation of miR-194 contributes to tumor growth and progression in pancreatic ductal adenocarcinoma". Oncology Reports 31.3 (2014): 1157-1164.
Chicago
Zhang, J., Zhao, C., Zhang, S., Yu, D., Chen, Y., Liu, Q., Shi, M., Ni, C., Zhu, M."Upregulation of miR-194 contributes to tumor growth and progression in pancreatic ductal adenocarcinoma". Oncology Reports 31, no. 3 (2014): 1157-1164. https://doi.org/10.3892/or.2013.2960
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Zhao C, Zhang S, Yu D, Chen Y, Liu Q, Shi M, Ni C and Zhu M: Upregulation of miR-194 contributes to tumor growth and progression in pancreatic ductal adenocarcinoma. Oncol Rep 31: 1157-1164, 2014.
APA
Zhang, J., Zhao, C., Zhang, S., Yu, D., Chen, Y., Liu, Q. ... Zhu, M. (2014). Upregulation of miR-194 contributes to tumor growth and progression in pancreatic ductal adenocarcinoma. Oncology Reports, 31, 1157-1164. https://doi.org/10.3892/or.2013.2960
MLA
Zhang, J., Zhao, C., Zhang, S., Yu, D., Chen, Y., Liu, Q., Shi, M., Ni, C., Zhu, M."Upregulation of miR-194 contributes to tumor growth and progression in pancreatic ductal adenocarcinoma". Oncology Reports 31.3 (2014): 1157-1164.
Chicago
Zhang, J., Zhao, C., Zhang, S., Yu, D., Chen, Y., Liu, Q., Shi, M., Ni, C., Zhu, M."Upregulation of miR-194 contributes to tumor growth and progression in pancreatic ductal adenocarcinoma". Oncology Reports 31, no. 3 (2014): 1157-1164. https://doi.org/10.3892/or.2013.2960
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