Expression of fatty acid binding protein 4 is involved in the cell growth of oral squamous cell carcinoma

Lee,Doksa; Wada,Koichiro; Taniguchi,Yoshitaka; Al-Shareef,Hani; Masuda,Tomotake; Usami,Yu; Aikawa,Tomonao; Okura,Masaya; Kamisaki,Yoshinori; Kogo,Mikihiko

doi:10.3892/or.2014.2975

Expression of fatty acid binding protein 4 is involved in the cell growth of oral squamous cell carcinoma

Authors:
- Doksa Lee
- Koichiro Wada
- Yoshitaka Taniguchi
- Hani Al-Shareef
- Tomotake Masuda
- Yu Usami
- Tomonao Aikawa
- Masaya Okura
- Yoshinori Kamisaki
- Mikihiko Kogo
View Affiliations

Affiliations: First Department of Oral and Maxillofacial Surgery, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan, Department of Pharmacology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan, Clinical Laboratory, Osaka University Dental Hospital, Suita, Osaka 565-0871, Japan
Published online on: January 13, 2014 https://doi.org/10.3892/or.2014.2975
Pages: 1116-1120

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Fatty acid binding proteins (FABPs) are a family of small and highly conserved lipid chaperone molecules with highly varied functions. Among them, fatty acid binding protein 4 (FABP4, also known as aP2) is highly expressed by adipocytes, macrophages and dendritic cells. Although the role of FABP4 in cancer is still unclear, it has been reported to be highly expressed by human tumors such as ovarian and bladder cancers. In the present study, we investigated the expression and role of FABP4 in oral squamous cell carcinoma (SCC) and its expression in oral SCC tissues. Immunohistochemical staining revealed that FABP4 expression in the tumor tissue was much higher than that in the non-tumor area of the same specimen. In the in vitro studies, an FABP4-knockdown SCC cell line (established through FABP4-specific siRNA) showed inhibited growth, and inhibited expression and activation of mitogen-activated protein kinase (MAPK). These results indicate that expression of FABP4 plays an important role in the cell growth of oral SCC through the MAPK pathway.

View Figures

View References

1	Prince S and Bailey BM: Squamous carcinoma of the tongue: review. Br J Oral Maxillofac Surg. 37:164–174. 1999. View Article : Google Scholar : PubMed/NCBI
2	Okura M, Hiranuma T, Adachi T, et al: Induction chemotherapy is associated with an increase in the incidence of locoregional recurrence in patients with carcinoma of the oral cavity: results from a single institution. Cancer. 82:804–815. 1998. View Article : Google Scholar : PubMed/NCBI
3	Goepfert H: Squamous cell carcinoma of the head and neck: past progress and future promise. CA Cancer J Clin. 48:195–198. 1998. View Article : Google Scholar : PubMed/NCBI
4	Schroeder F, Petrescu AD, Huang H, et al: Role of fatty acid binding proteins and long chain fatty acids in modulating nuclear receptors and gene transcription. Lipids. 43:1–17. 2008. View Article : Google Scholar : PubMed/NCBI
5	Furuhashi M and Hotamisligil GS: Fatty acid-binding proteins: role in metabolic diseases and potential as drug targets. Nat Rev Drug Discov. 7:489–503. 2008. View Article : Google Scholar : PubMed/NCBI
6	Storch J and Thumser AE: The fatty acid transport function of fatty acid-binding proteins. Biochim Biophys Acta. 1486:28–44. 2000. View Article : Google Scholar : PubMed/NCBI
7	Hotamisligil GS, Johnson RS, Distel RJ, Ellis R, Papaioannou VE and Spiegelman BM: Uncoupling of obesity from insulin resistance through a targeted mutation in aP2, the adipocyte fatty acid binding protein. Science. 274:1377–1379. 1996. View Article : Google Scholar : PubMed/NCBI
8	Uysal KT, Scheja L, Wiesbrock SM, Bonner-Weir S and Hotamisligil GS: Improved glucose and lipid metabolism in genetically obese mice lacking aP2. Endocrinology. 141:3388–3396. 2000. View Article : Google Scholar : PubMed/NCBI
9	Makowski L, Boord JB, Maeda K, et al: Lack of macrophage fatty-acid-binding protein aP2 protects mice deficient in apolipoprotein E against atherosclerosis. Nat Med. 7:699–705. 2001. View Article : Google Scholar : PubMed/NCBI
10	Nieman KM, Kenny HA, Penicka CV, et al: Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth. Nat Med. 17:1498–1503. 2011. View Article : Google Scholar : PubMed/NCBI
11	Masuda T, Wada K, Nakajima A, et al: Critical role of peroxisome proliferator-activated receptor γ on anoikis and invasion of squamous cell carcinoma. Clin Cancer Res. 11:4012–4021. 2005.
12	Nagata M, Wada K, Nakajima A, et al: Role of myeloid cell leukemia-1 in cell growth of squamous cell carcinoma. J Pharmacol Sci. 110:344–353. 2009. View Article : Google Scholar : PubMed/NCBI
13	Kusayama M, Wada K, Nagata M, et al: Critical role of aquaporin 3 on growth of human esophageal and oral squamous cell carcinoma. Cancer Sci. 102:1128–1136. 2011. View Article : Google Scholar : PubMed/NCBI
14	Ishimoto S, Wada K, Tanaka N, et al: Role of endothelin receptor signalling in squamous cell carcinoma. Int J Oncol. 40:1011–1019. 2012.PubMed/NCBI
15	Ishimoto S, Wada K, Usami Y, et al: Differential expression of aquaporin 5 and aquaporin 3 in squamous cell carcinoma and adenoid cystic carcinoma. Int J Oncol. 41:67–75. 2012.PubMed/NCBI
16	Tan NS, Shaw NS, Vinckenbosch N, et al: Selective cooperation between fatty acid binding proteins and peroxisome proliferator-activated receptors in regulating transcription. Mol Cell Biol. 22:5114–5127. 2002. View Article : Google Scholar
17	Ishida H, Wada K, Masuda T, et al: Critical role of estrogen receptor on anoikis and invasion of squamous cell carcinoma. Cancer Sci. 98:636–643. 2007. View Article : Google Scholar : PubMed/NCBI
18	Takahashi H, Fujita K, Fujisawa T, et al: Inhibition of peroxisome proliferator-activated receptor gamma activity in esophageal carcinoma cells results in a drastic decrease of invasive properties. Cancer Sci. 97:854–860. 2006. View Article : Google Scholar
19	Roux PP and Blenis J: ERK and p38 MAPK-activated protein kinases: a family of protein kinases with diverse biological functions. Microbiol Mol Biol Rev. 68:320–344. 2004. View Article : Google Scholar : PubMed/NCBI
20	Roberts PJ and Der CJ: Targeting the Raf-MEK-ERK mitogen-activated protein kinase cascade for the treatment of cancer. Oncogene. 26:3291–3310. 2007. View Article : Google Scholar : PubMed/NCBI
21	Santarpia L, Lippman SM and El-Naggar AK: Targeting the MAPK-RAS-RAF signaling pathway in cancer therapy. Expert Opin Ther Targets. 16:103–119. 2012. View Article : Google Scholar : PubMed/NCBI
22	Boiteux G, Lascombe I, Roche E, et al: A-FABP, a candidate progression marker of human transitional cell carcinoma of the bladder, is differentially regulated by PPAR in urothelial cancer cells. Int J Cancer. 124:1820–1828. 2009. View Article : Google Scholar : PubMed/NCBI
23	Uma RS, Naresh KN, D’Cruz AK, Mulherkar R and Borges AM: Metastasis of squamous cell carcinoma of the oral tongue is associated with down-regulation of epidermal fatty acid binding protein (E-FABP). Oral Oncol. 43:27–32. 2007. View Article : Google Scholar : PubMed/NCBI
24	Fang LY, Wong TY, Chiang WF and Chen YL: Fatty-acid-binding protein 5 promotes cell proliferation and invasion in oral squamous cell carcinoma. J Oral Pathol Med. 39:342–348. 2010. View Article : Google Scholar : PubMed/NCBI