Analysis of the B-RafV600E mutation in cutaneous melanoma patients with occupational sun exposure

Candido,Saverio; Rapisarda,Venerando; Marconi,Andrea; Malaponte,Grazia; Bevelacqua,Valentina; Gangemi,Pietro; Scalisi,Aurora; McCubrey,James A.; Maestro,Roberta; Spandidos,Demetrios A.; Fenga,Concettina; Libra,Massimo

doi:10.3892/or.2014.2977

Analysis of the B-RafV600E mutation in cutaneous melanoma patients with occupational sun exposure

Authors:
- Saverio Candido
- Venerando Rapisarda
- Andrea Marconi
- Grazia Malaponte
- Valentina Bevelacqua
- Pietro Gangemi
- Aurora Scalisi
- James A. McCubrey
- Roberta Maestro
- Demetrios A. Spandidos
- Concettina Fenga
- Massimo Libra
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Affiliations: Laboratory of Translational Oncology and Functional Genomics, Section of General Pathology and Oncology, Department of Bio-medical Sciences, University of Catania, Catania 95124, Italy, Occupational Medicine, Vittorio Emanuele - Policlinico Hospital, University of Catania, Catania 95100, Italy, Section of Occupational Medicine, Department of the Environment, Security, Territory, Food and Health Sciences, University of Messina, Messina 98125, Italy, Division of Pathology, Vittorio Emanuele - Policlinico Hospital, University of Catania, Catania 95100, Italy, Unit of Oncologic Diseases, ASP-Catania, Catania 95100, Italy, Department of Microbiology and Immunology, East Carolina University, Greenville, NC, USA, Experimental Oncology 1, CRO National Cancer Institute, Aviano, Italy, Department of Virology, Medical School, University of Crete, Heraklion 71003, Crete, Greece
Published online on: January 14, 2014 https://doi.org/10.3892/or.2014.2977
Pages: 1079-1082
Copyright: © Candido et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Sun-exposure is one of the risk factors associated with the development of a cutaneous neoplasm. In melanoma, the Ras-Raf-MEK-ERK (MAPK) signaling pathway is constitutively activated through multiple mechanisms, including B-Raf mutation. It has been hypothesized that B-Raf mutations in melanocytic lesions arise from DNA damage induced by ultraviolet (UV) radiation. However, it is still discussed if B-Raf mutations are associated with melanoma patients exposed to the sun. Therefore, in the present study, the known B-RafV600E mutation was analysed in melanoma samples from 30 indoor and 38 outdoor workers. B-RafV600E mutation was detected in 52 and 73% of outdoor workers and indoor workers, respectively. Of note, this mutation was identified in 12 of 14 (85%) melanoma of the trunk diagnosed in indoor workers and in 9 of 19 (47%) samples from outdoor workers (p=0.03). By analyzing melanomas of other body sites, no statistical difference in the frequency of B-RafV600E mutation was identified between the groups of workers. It appears that the mutation detected among indoor workers may be associated with a recreational or intermittent exposure to the sun, as usually the trunk is a sun-protected body site. Overall, these data indicate that the B-RafV600E mutation detected in melanoma is not associated with a chronic exposure to the sun. Mutations detected in other genes may also contribute to melanoma development in the subset of patients exposed to UV radiation.

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