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2014-March Volume 31 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

siRNA-mediated knockdown of hTDE2 retards cell cycle progression through transcriptional activation of p21

  • Authors:
    • Wei-Hua Ren
    • Chen-Yi Yang
    • Xian-Mei Yang
    • Long Yu
  • View Affiliations / Copyright

    Affiliations: The State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, P.R. China
  • Pages: 1314-1322
    |
    Published online on: January 14, 2014
       https://doi.org/10.3892/or.2014.2980
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Abstract

Carcinogenesis is a very complex process involving a series of changes of tumor-related genes. Therefore, genes differentially expressed in tumors have received significant attention. Among them is the tumor differentially expressed (TDE) protein family, which shows no homologue to any other protein families and is unique to eukaryotes. The members of the TDE (also known as Serinc) family are highly conserved, showing approximately 30-80% homologue of their amino acid sequences. Previous reports have shown that both human and mice TDE/Serinc proteins are always upregulated in carcinomatous tissues. However, their precise physiological roles remain unclear. The human TDE2/Serinc1 gene was cloned by our laboratory during the screening for differentially expressed genes in hepatocarcinoma. In the present study, we knocked down the expression of TDE2 with specific siRNA fragments in two human hepatocarcinoma cell lines, and this caused cell cycle arrest at G2. Cell cycle progression is monitored and regulated by several factors. p21, the cdk inhibitor, is a key player and could be transcriptionally activated by many factors including sterol regulatory element-binding proteins (SREBPs). Previous research demonstrated that rat TDE2 could facilitate the cellular sphingolipids biosynthesis in both yeast and mammalian cells. Therefore, we further analyzed the effect of TDE2 knockdown on p21 and SREBP, and found that endogenous p21 expression was upregulated, as was that of SREBPs (-1a and 2). In conclusion, our preliminary results indicated that TDE2 may have an effect on tumor cell growth by influencing the expression of SREBP and p21.
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Copy and paste a formatted citation
Spandidos Publications style
Ren W, Yang C, Yang X and Yu L: siRNA-mediated knockdown of hTDE2 retards cell cycle progression through transcriptional activation of p21. Oncol Rep 31: 1314-1322, 2014.
APA
Ren, W., Yang, C., Yang, X., & Yu, L. (2014). siRNA-mediated knockdown of hTDE2 retards cell cycle progression through transcriptional activation of p21. Oncology Reports, 31, 1314-1322. https://doi.org/10.3892/or.2014.2980
MLA
Ren, W., Yang, C., Yang, X., Yu, L."siRNA-mediated knockdown of hTDE2 retards cell cycle progression through transcriptional activation of p21". Oncology Reports 31.3 (2014): 1314-1322.
Chicago
Ren, W., Yang, C., Yang, X., Yu, L."siRNA-mediated knockdown of hTDE2 retards cell cycle progression through transcriptional activation of p21". Oncology Reports 31, no. 3 (2014): 1314-1322. https://doi.org/10.3892/or.2014.2980
Copy and paste a formatted citation
x
Spandidos Publications style
Ren W, Yang C, Yang X and Yu L: siRNA-mediated knockdown of hTDE2 retards cell cycle progression through transcriptional activation of p21. Oncol Rep 31: 1314-1322, 2014.
APA
Ren, W., Yang, C., Yang, X., & Yu, L. (2014). siRNA-mediated knockdown of hTDE2 retards cell cycle progression through transcriptional activation of p21. Oncology Reports, 31, 1314-1322. https://doi.org/10.3892/or.2014.2980
MLA
Ren, W., Yang, C., Yang, X., Yu, L."siRNA-mediated knockdown of hTDE2 retards cell cycle progression through transcriptional activation of p21". Oncology Reports 31.3 (2014): 1314-1322.
Chicago
Ren, W., Yang, C., Yang, X., Yu, L."siRNA-mediated knockdown of hTDE2 retards cell cycle progression through transcriptional activation of p21". Oncology Reports 31, no. 3 (2014): 1314-1322. https://doi.org/10.3892/or.2014.2980
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