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2014-April Volume 31 Issue 4

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Article

Serum levels of matrix metalloproteinase-9 predict lymph node metastasis in breast cancer patients

  • Authors:
    • Dong Sik Heo
    • Hoon Choi
    • Mi Young Yeom
    • Byung Joo Song
    • Se Jeong Oh
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Incheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Incheon, Republic of Korea, Department of Clinical Research Laboratory, Incheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Incheon, Republic of Korea, Department of Surgery, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
  • Pages: 1567-1572
    |
    Published online on: January 27, 2014
       https://doi.org/10.3892/or.2014.3001
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Abstract

Matrix metalloproteinases (MMPs) are proteolytic enzymes that play important roles in cancer progression and metastasis. Although serum MMP expression is known to correlate with the primary lesion of breast cancer, there has yet to be a study regarding the correlation between serum MMP expression and metastatic lesions, particularly lymph nodes. The present study evaluated the correlation of serum and lymph node MMP expression with axillary node metastasis. The preoperative serum levels of MMP-2 and MMP-9 in 77 patients with breast cancer and in 10 patients with benign breast tumor were determined by ELISA and zymography. One hundred and twelve axillary lymph nodes were collected for zymography during breast cancer surgery. Significantly higher serum levels of MMP-2 and MMP-9 were found in breast cancer patients compared to patients with benign tumor. High serum levels of MMP-2 and MMP-9 were significantly associated with node metastasis. ELISA and zymography results for serum MMP-2 and MMP-9 correlated significantly, with a Pearson correlation coefficient (r) of 0.76 for MMP-2 (P=0.001) and 0.81 for MMP-9 (P=0.001). In terms of lymph node, total MMP-2, MMP-9 and MMP-9 activity were significantly higher in metastatic than in non-metastatic nodes. There was a correlation between serum and lymph node MMP-9 levels on zymographic measurements (r=0.34, P=0.011), but not in terms of MMP-2 levels. Serum MMP-9 levels may have a diagnostic value for predicting axillary node metastasis.
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Copy and paste a formatted citation
Spandidos Publications style
Heo DS, Choi H, Yeom MY, Song BJ and Oh SJ: Serum levels of matrix metalloproteinase-9 predict lymph node metastasis in breast cancer patients. Oncol Rep 31: 1567-1572, 2014.
APA
Heo, D.S., Choi, H., Yeom, M.Y., Song, B.J., & Oh, S.J. (2014). Serum levels of matrix metalloproteinase-9 predict lymph node metastasis in breast cancer patients. Oncology Reports, 31, 1567-1572. https://doi.org/10.3892/or.2014.3001
MLA
Heo, D. S., Choi, H., Yeom, M. Y., Song, B. J., Oh, S. J."Serum levels of matrix metalloproteinase-9 predict lymph node metastasis in breast cancer patients". Oncology Reports 31.4 (2014): 1567-1572.
Chicago
Heo, D. S., Choi, H., Yeom, M. Y., Song, B. J., Oh, S. J."Serum levels of matrix metalloproteinase-9 predict lymph node metastasis in breast cancer patients". Oncology Reports 31, no. 4 (2014): 1567-1572. https://doi.org/10.3892/or.2014.3001
Copy and paste a formatted citation
x
Spandidos Publications style
Heo DS, Choi H, Yeom MY, Song BJ and Oh SJ: Serum levels of matrix metalloproteinase-9 predict lymph node metastasis in breast cancer patients. Oncol Rep 31: 1567-1572, 2014.
APA
Heo, D.S., Choi, H., Yeom, M.Y., Song, B.J., & Oh, S.J. (2014). Serum levels of matrix metalloproteinase-9 predict lymph node metastasis in breast cancer patients. Oncology Reports, 31, 1567-1572. https://doi.org/10.3892/or.2014.3001
MLA
Heo, D. S., Choi, H., Yeom, M. Y., Song, B. J., Oh, S. J."Serum levels of matrix metalloproteinase-9 predict lymph node metastasis in breast cancer patients". Oncology Reports 31.4 (2014): 1567-1572.
Chicago
Heo, D. S., Choi, H., Yeom, M. Y., Song, B. J., Oh, S. J."Serum levels of matrix metalloproteinase-9 predict lymph node metastasis in breast cancer patients". Oncology Reports 31, no. 4 (2014): 1567-1572. https://doi.org/10.3892/or.2014.3001
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