Nuclear-cytoplasmic PARP-1 expression as an unfavorable prognostic marker in lymph node‑negative early breast cancer: 15-year follow-up

  • Authors:
    • Piotr Donizy
    • Grazyna Pietrzyk
    • Agnieszka Halon
    • Cyprian Kozyra
    • Tserenchunt Gansukh
    • Hermann Lage
    • Pawel Surowiak
    • Rafal Matkowski
  • View Affiliations

  • Published online on: February 18, 2014     https://doi.org/10.3892/or.2014.3024
  • Pages: 1777-1787
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Abstract

PARP-1 plays an important role in DNA damage repair and maintaining genome integrity by repairing DNA single-strand breaks (SSBs) by base excision repair (BER). The aim of the present study was to examine the expression of PARP-1 in breast cancer (BC) patients and to assess the relationship between the subcellular localization of this protein and clinicopathological characteristics. The reactivity of PARP-1 was analyzed by immunohistochemistry in a homogeneous group of 83 stage II ductal BC patients with a 15-year follow-up. Immunostaining of PARP-1 was also evaluated in 4 human BC cell lines and resistance prediction profile for 11 anticancer agents was performed using 3 models of drug-resistant cell lines. Nuclear-cytoplasmic expression (NCE) was associated with shorter overall survival, which was not statistically significant during the 10-year follow-up but became statistically significant after 10 years of observation, during the 15-year follow-up (P=0.015). Analysis performed in subgroups of patients with (N+) and without (N-) nodal metastases showed that NCE was associated with poor clinical outcome in N- patients (P=0.017). Multivariate analysis confirmed a significant impact of NCE on unfavorable prognosis in N- early BC. The presence of PARP-1 NCE may be a new potential unfavorable prognostic factor in lymph node- negative early BC.
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2014-April
Volume 31 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Donizy P, Pietrzyk G, Halon A, Kozyra C, Gansukh T, Lage H, Surowiak P and Matkowski R: Nuclear-cytoplasmic PARP-1 expression as an unfavorable prognostic marker in lymph node‑negative early breast cancer: 15-year follow-up. Oncol Rep 31: 1777-1787, 2014
APA
Donizy, P., Pietrzyk, G., Halon, A., Kozyra, C., Gansukh, T., Lage, H. ... Matkowski, R. (2014). Nuclear-cytoplasmic PARP-1 expression as an unfavorable prognostic marker in lymph node‑negative early breast cancer: 15-year follow-up. Oncology Reports, 31, 1777-1787. https://doi.org/10.3892/or.2014.3024
MLA
Donizy, P., Pietrzyk, G., Halon, A., Kozyra, C., Gansukh, T., Lage, H., Surowiak, P., Matkowski, R."Nuclear-cytoplasmic PARP-1 expression as an unfavorable prognostic marker in lymph node‑negative early breast cancer: 15-year follow-up". Oncology Reports 31.4 (2014): 1777-1787.
Chicago
Donizy, P., Pietrzyk, G., Halon, A., Kozyra, C., Gansukh, T., Lage, H., Surowiak, P., Matkowski, R."Nuclear-cytoplasmic PARP-1 expression as an unfavorable prognostic marker in lymph node‑negative early breast cancer: 15-year follow-up". Oncology Reports 31, no. 4 (2014): 1777-1787. https://doi.org/10.3892/or.2014.3024