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2014-April Volume 31 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Validation of histological diagnostic methods for detecting endothelin B receptor expression

  • Authors:
    • Naoya Fukuda
    • Takahiro Tsuchikawa
    • Akira Fukunaga
    • Hiroshi Kawase
    • Naotake Homma
    • Toru Nakamura
    • Toshiaki Shichinohe
    • Satoshi Hirano
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Pages: 1561-1566
    |
    Published online on: February 19, 2014
       https://doi.org/10.3892/or.2014.3031
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Abstract

Pancreatic ductal adenocarcinoma (PDAC) has an extremely poor prognosis. Recently, it was reported that the endothelin B receptor (ETBR) of tumor endothelial cells prevents antitumor immunity. However, the immuno­histochemistry (IHC) conditions required to detect ETBR expression remain unclear. The aim of the present study was to confirm the appropriate conditions for IHC for ETBR using ETBR cDNA and transfectant cells and to assess ETBR expression in PDAC patients. An ETBR-expressing cell was established as an objective positive control and the detectability of ETBR expression was evaluated using several types of anti-ETBR antibodies. ETBR mRNA expression was then studied. Finally, ETBR expression was examined in human PDAC tissue using IHC. As a result, four different anti-ETBR antibodies recognized the cell surface ETBR appropriately. A non-specific reaction was shown in the detection of ETBR in normal human tissues. ETBR mRNA expression was weakly detected only in the adrenal gland. No biologically significant correlation was observed in the ETBR-IHC of human PDAC sections. In conclusion, it is necessary to perform IHC using an appropriate control to assess the tissue expression of ETBR.
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Copy and paste a formatted citation
Spandidos Publications style
Fukuda N, Tsuchikawa T, Fukunaga A, Kawase H, Homma N, Nakamura T, Shichinohe T and Hirano S: Validation of histological diagnostic methods for detecting endothelin B receptor expression. Oncol Rep 31: 1561-1566, 2014.
APA
Fukuda, N., Tsuchikawa, T., Fukunaga, A., Kawase, H., Homma, N., Nakamura, T. ... Hirano, S. (2014). Validation of histological diagnostic methods for detecting endothelin B receptor expression. Oncology Reports, 31, 1561-1566. https://doi.org/10.3892/or.2014.3031
MLA
Fukuda, N., Tsuchikawa, T., Fukunaga, A., Kawase, H., Homma, N., Nakamura, T., Shichinohe, T., Hirano, S."Validation of histological diagnostic methods for detecting endothelin B receptor expression". Oncology Reports 31.4 (2014): 1561-1566.
Chicago
Fukuda, N., Tsuchikawa, T., Fukunaga, A., Kawase, H., Homma, N., Nakamura, T., Shichinohe, T., Hirano, S."Validation of histological diagnostic methods for detecting endothelin B receptor expression". Oncology Reports 31, no. 4 (2014): 1561-1566. https://doi.org/10.3892/or.2014.3031
Copy and paste a formatted citation
x
Spandidos Publications style
Fukuda N, Tsuchikawa T, Fukunaga A, Kawase H, Homma N, Nakamura T, Shichinohe T and Hirano S: Validation of histological diagnostic methods for detecting endothelin B receptor expression. Oncol Rep 31: 1561-1566, 2014.
APA
Fukuda, N., Tsuchikawa, T., Fukunaga, A., Kawase, H., Homma, N., Nakamura, T. ... Hirano, S. (2014). Validation of histological diagnostic methods for detecting endothelin B receptor expression. Oncology Reports, 31, 1561-1566. https://doi.org/10.3892/or.2014.3031
MLA
Fukuda, N., Tsuchikawa, T., Fukunaga, A., Kawase, H., Homma, N., Nakamura, T., Shichinohe, T., Hirano, S."Validation of histological diagnostic methods for detecting endothelin B receptor expression". Oncology Reports 31.4 (2014): 1561-1566.
Chicago
Fukuda, N., Tsuchikawa, T., Fukunaga, A., Kawase, H., Homma, N., Nakamura, T., Shichinohe, T., Hirano, S."Validation of histological diagnostic methods for detecting endothelin B receptor expression". Oncology Reports 31, no. 4 (2014): 1561-1566. https://doi.org/10.3892/or.2014.3031
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