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Article

Livistona chinensis seeds inhibit hepatocellular carcinoma angiogenesis in vivo via suppression of the Notch pathway

  • Authors:
    • Wei Lin
    • Jinyan Zhao
    • Zhiyun Cao
    • Qunchuan Zhuang
    • Liangpu Zheng
    • Jianwei Zeng
    • Zhenfeng Hong
    • Jun Peng
  • View Affiliations / Copyright

    Affiliations: Academy of Integrative Medicine Biomedical Research Center, Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian 350122, P.R. China
  • Pages: 1723-1728
    |
    Published online on: February 24, 2014
       https://doi.org/10.3892/or.2014.3051
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Abstract

Livistona chinensis seeds have been used for centuries to clinically treat various types of cancer. Our published data suggest that Livistona chinensis seeds are able to inhibit hepatocellular carcinoma (HCC) growth in vitro and in vivo via promotion of mitochondrial-dependent apoptosis. To further elucidate the molecular mechanisms of its antitumor activity, in the present study, we used an HCC xenograft mouse model to evaluate the effect of an ethanol extract of Livistona chinensis seeds (EELC) on tumor angiogenesis and on the activation of the Notch pathway. Intratumoral microvessel density (MVD) in HCC xenograft mouse tumors was evaluated via immunohistochemical (IHC) staining for CD31. The mRNA and protein expression of vascular endothelial growth factor A (VEGF-A), VEGFR-2, Notch, Dll4 and Jagged1 was evaluated using RT-PCR and IHC, respectively. We found that EELC profoundly reduced MVD in the HCC mouse tumors, demonstrating the in vivo inhibitory effect of EELC on tumor angiogenesis. In addition, EELC treatment reduced the expression of VEGF-A and VEGFR-2 in tumor tissues. Furthermore, EELC treatment inhibited the expression of Notch, Dll4 and Jagged1. Our findings suggest that Livistona chinensis seeds inhibit tumor angiogenesis through suppression of the Notch pathway.
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1 

Parkin DM, Bray F, Ferlay J and Pisani P: Global cancer statistics, 2002. CA Cancer J Clin. 55:74–108. 2005. View Article : Google Scholar

2 

Lang H, Sotiropoulos GC, Brokalaki EI, Schmitz KJ, Bertona C, Meyer G, Frilling A, Paul A, Malagó M and Broelsch CE: Survival and recurrence rates after resection for hepatocellular carcinoma in noncirrhotic livers. J Am Coll Surg. 205:27–36. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Gish RG and Baron A: Hepatocellular carcinoma (HCC): current and evolving therapies. IDrugs. 11:198–203. 2008.PubMed/NCBI

4 

Gordaliza M: Natural products as leads to anticancer drugs. Clin Transl Oncol. 9:767–776. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Jia L: Cancer complementary and alternative medicine research at the US National Cancer Institute. Chin J Integr Med. 18:325–332. 2012. View Article : Google Scholar

6 

Carmady B and Smith CA: Use of Chinese medicine by cancer patients: a review of surveys. Chin Med. 9:222011. View Article : Google Scholar

7 

Cheueng S and Tai J: In vitro studies of the dry fruit of Chinese fan palm Livistona chinensis. Oncol Rep. 5:1331–1336. 2005.

8 

Sartippour MR, Liu C and Shao ZM: Livistona extract inhibits angiogenesis and cancer growth. Oncol Rep. 6:1355–1357. 2001.

9 

Huang WC, Hsu RM, Chi LM, et al: Selective downregulation of EGF receptor and downstream MAPK pathway in human cancer cell lines by active components partially purified from the seeds of Livistona chinensis R. Brown. Cancer Lett. 248:137–146. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Wang H, Li A, Dong XP and Xu XY: Screening of anti-tumor parts from the seeds of Livistona chinensis and its anti-angiogenesis effect. Zhong Yao Cai. 31:718–722. 2008.(In Chinese).

11 

Lin W, Zhao J, Cao Z, Zhuang Q, Zheng L, Cai Q, Chen D, Wang L, Hong Z and Peng J: Livistona chinensis seed suppresses hepatocellular carcinoma growth through promotion of mitochondrial-dependent apoptosis. Oncol Rep. 29:1859–1866. 2013.

12 

Folkman J and Klagsbrun M: Angiogenic factors. Science. 235:442–447. 1987. View Article : Google Scholar

13 

Folkman J: Tumor angiogenesis: therapeutic implications. N Engl J Med. 285:1182–1186. 1971. View Article : Google Scholar : PubMed/NCBI

14 

Schneider BP and Miller KD: Angiogenesis of breast cancer. J Clin Oncol. 23:1782–1790. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Uzzan B, Nicolas P, Cucherat M and Perret GY: Microvessel density as a prognostic factor in women with breast cancer: a systematic review of the literature and meta-analysis. Cancer Res. 64:2941–2955. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Weidner N: The importance of tumor angiogenesis: the evidence continues to grow. Am J Clin Pathol. 122:675–677. 2004. View Article : Google Scholar : PubMed/NCBI

17 

Kut C, Mac Gabhann F and Popel AS: Where is VEGF in the body? A meta-analysis of VEGF distribution in cancer. Br J Cancer. 97:978–985. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Takeshita K, Satoh M, Ii M, Silver M, Limbourg FP, Mukai Y, Rikitake Y, Radtke F, Gridley T, Losordo DW and Liao JK: Critical role of endothelial Notch1 signaling in postnatal angiogenesis. Circ Res. 100:70–78. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Garcia A and Kandel JJ: Notch: a key regulator of tumor angiogenesis and metastasis. Histol Histopathol. 27:151–156. 2012.PubMed/NCBI

20 

Dufraine J, Funahashi Y and Kitajewski J: Notch signaling regulates tumor angiogenesis by diverse mechanisms. Oncogene. 27:5132–5137. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Ridgway J, Zhang G, Wu Y, Stawicki S, Liang WC, Chanthery Y, Kowalski J, Watts RJ, Callahan C, Kasman I, Singh M, Chien M, Tan C, Hongo JA, de Sauvage F, Plowman G and Yan M: Inhibition of Dll4 signalling inhibits tumour growth by deregulating angiogenesis. Nature. 444:1083–1087. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Miele L, Golde T and Osborne B: Notch signaling in cancer. Curr Mol Med. 6:905–918. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Yanagawa S, Lee JS, Kakimi K, Matsuda Y, Honjo T and Ishimoto A: Identification of Notch1 as a frequent target for provirus insertional mutagenesis in T-cell lymphomas induced by leukemogenic mutants of mouse mammary tumor virus. J Virol. 74:9786–9791. 2000.

24 

Ellisen LW, Bird J, West DC, Soreng AL, Reynolds TC, Smith SD and Sklar J: TAN-1, the human homolog of the Drosophila Notch gene, is broken by chromosomal translocations in T lymphoblastic neoplasms. Cell. 66:649–661. 1991. View Article : Google Scholar

25 

Sjölund J, Manetopoulos C, Stockhausen MT and Axelson H: The Notch pathway in cancer: differentiation gone awry. Eur J Cancer. 41:2620–2629. 2005.PubMed/NCBI

26 

Nijjar SS, Crosby HA, Wallace L, Hubscher SG and Strain AJ: Notch receptor expression in adult human liver: a possible role in bile duct formation and hepatic neovascularization. Hepatology. 34:1184–1192. 2001. View Article : Google Scholar

27 

Nijjar SS, Wallace L, Crosby HA, Hubscher SG and Strain AJ: Altered Notch ligand expression in human liver disease: further evidence for a role of the Notch signaling pathway in hepatic neovascularization and biliary ductular defects. Am J Pathol. 160:1695–1703. 2002. View Article : Google Scholar

28 

Dorrell MI, Aguilar E, Scheppke L, Barnett FH and Friedlander M: Combination angiostatic therapy completely inhibits ocular and tumor angiogenesis. Proc Natl Acad Sci USA. 104:967–972. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Folkman J and Shing Y: Angiogenesis. J Biol Chem. 267:10931–10934. 1992.

30 

Folkman J: Angiogenesis in cancer, vascular, rheumatoid and other disease. Nat Med. 1:27–31. 1995. View Article : Google Scholar : PubMed/NCBI

31 

Folkman J: Angiogenesis. Annu Rev Med. 57:1–18. 2006. View Article : Google Scholar

32 

Weidner N, Semple JP, Welch WR and Folkman J: Tumor angiogenesis and metastasis - correlation in invasive breast carcinoma. N Engl J Med. 324:1–8. 1991. View Article : Google Scholar : PubMed/NCBI

33 

Risau W: Mechanisms of angiogenesis. Nature. 386:671–674. 1997. View Article : Google Scholar : PubMed/NCBI

34 

Jain RK: Tumor angiogenesis and accessibility: role of vascular endothelial growth factor. Semin Oncol. 29(Suppl 16): 3–9. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Ferrara N: Role of vascular endothelial growth factor in physiologic and pathologic angiogenesis: therapeutic implications. Semin Oncol. 29(Suppl 16): 10–14. 2002. View Article : Google Scholar : PubMed/NCBI

36 

Maeda K, Chung YS, Ogawa Y, Takatsuka S, Kang SM, Ogawa M, Sawada T and Sowa M: Prognostic value of vascular endothelial growth factor expression in gastric carcinoma. Cancer. 77:858–863. 1996. View Article : Google Scholar : PubMed/NCBI

37 

Kaya M, Wada T, Akatsuka T, Kawaguchi S, Nagoya S, Shindoh M, Higashino F, Mezawa F, Okada F and Ishii S: Vascular endothelial growth factor expression in untreated osteosarcoma is predictive of pulmonary metastasis and poor prognosis. Clin Cancer Res. 6:572–577. 2000.PubMed/NCBI

38 

Ferrara N, Gerber HP and LeCouter J: The biology of VEGF and its receptors. Nat Med. 9:669–676. 2003. View Article : Google Scholar : PubMed/NCBI

39 

Purow B: Notch inhibition as a promising new approach to cancer therapy. Adv Exp Med Biol. 727:305–319. 2012. View Article : Google Scholar : PubMed/NCBI

40 

Gale NW, Dominguez MG, Noguera I, Pan L, Hughes V, Valenzuela DM, Murphy AJ, Adams NC, Lin HC, Holash J, Thurston G and Yancopoulos GD: Haploinsufficiency of delta-like 4 ligand results in embryonic lethality due to major defects in arterial and vascular development. Proc Natl Acad Sci USA. 101:15949–15954. 2004. View Article : Google Scholar : PubMed/NCBI

41 

Duarte A, Hirashima M, Benedito R, Trindade A, Diniz P, Bekman E, Costa L, Henrique D and Rossant J: Dosage-sensitive requirement for mouse Dll4 in artery development. Genes Dev. 18:2474–2478. 2004. View Article : Google Scholar : PubMed/NCBI

42 

Skrtic A, Sokolic L, Borovecki A, Rosa J and Fenzl V: Immunohistochemical localization of CD31, NOTCH1 and JAGGED1 proteins in experimentally induced polycystic ovaries of immature rats. Acta Histochem. 113:262–269. 2011. View Article : Google Scholar : PubMed/NCBI

43 

Benedito R, Roca C, Sörensen I, Adams S, Gossler A, Fruttiger M and Adams RH: The notch ligands Dll4 and Jagged1 have opposing effects on angiogenesis. Cell. 137:1124–1135. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lin W, Zhao J, Cao Z, Zhuang Q, Zheng L, Zeng J, Hong Z and Peng J: Livistona chinensis seeds inhibit hepatocellular carcinoma angiogenesis in vivo via suppression of the Notch pathway. Oncol Rep 31: 1723-1728, 2014.
APA
Lin, W., Zhao, J., Cao, Z., Zhuang, Q., Zheng, L., Zeng, J. ... Peng, J. (2014). Livistona chinensis seeds inhibit hepatocellular carcinoma angiogenesis in vivo via suppression of the Notch pathway. Oncology Reports, 31, 1723-1728. https://doi.org/10.3892/or.2014.3051
MLA
Lin, W., Zhao, J., Cao, Z., Zhuang, Q., Zheng, L., Zeng, J., Hong, Z., Peng, J."Livistona chinensis seeds inhibit hepatocellular carcinoma angiogenesis in vivo via suppression of the Notch pathway". Oncology Reports 31.4 (2014): 1723-1728.
Chicago
Lin, W., Zhao, J., Cao, Z., Zhuang, Q., Zheng, L., Zeng, J., Hong, Z., Peng, J."Livistona chinensis seeds inhibit hepatocellular carcinoma angiogenesis in vivo via suppression of the Notch pathway". Oncology Reports 31, no. 4 (2014): 1723-1728. https://doi.org/10.3892/or.2014.3051
Copy and paste a formatted citation
x
Spandidos Publications style
Lin W, Zhao J, Cao Z, Zhuang Q, Zheng L, Zeng J, Hong Z and Peng J: Livistona chinensis seeds inhibit hepatocellular carcinoma angiogenesis in vivo via suppression of the Notch pathway. Oncol Rep 31: 1723-1728, 2014.
APA
Lin, W., Zhao, J., Cao, Z., Zhuang, Q., Zheng, L., Zeng, J. ... Peng, J. (2014). Livistona chinensis seeds inhibit hepatocellular carcinoma angiogenesis in vivo via suppression of the Notch pathway. Oncology Reports, 31, 1723-1728. https://doi.org/10.3892/or.2014.3051
MLA
Lin, W., Zhao, J., Cao, Z., Zhuang, Q., Zheng, L., Zeng, J., Hong, Z., Peng, J."Livistona chinensis seeds inhibit hepatocellular carcinoma angiogenesis in vivo via suppression of the Notch pathway". Oncology Reports 31.4 (2014): 1723-1728.
Chicago
Lin, W., Zhao, J., Cao, Z., Zhuang, Q., Zheng, L., Zeng, J., Hong, Z., Peng, J."Livistona chinensis seeds inhibit hepatocellular carcinoma angiogenesis in vivo via suppression of the Notch pathway". Oncology Reports 31, no. 4 (2014): 1723-1728. https://doi.org/10.3892/or.2014.3051
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