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2014-April Volume 31 Issue 4

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Gene profiling and bioinformatics analyses reveal time course differential gene expression in surgically resected colorectal tissues

  • Authors:
    • Aya Yamagishi
    • Satoshi Matsumoto
    • Atsushi Watanabe
    • Yoshiaki Mizuguchi
    • Keisuke Hara
    • Hayato Kan
    • Takeshi Yamada
    • Michihiro Koizumi
    • Seiichi Shinji
    • Akihisa Matsuda
    • Junpei Sasaki
    • Takashi Shimada
    • Eiji Uchida
  • View Affiliations / Copyright

    Affiliations: Surgery for Organ Function and Biological Regulation, Graduate School of Medicine, Nippon Medical School, Tokyo 113-8603, Japan, Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo 113-8603, Japan
    Copyright: © Yamagishi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1531-1538
    |
    Published online on: February 24, 2014
       https://doi.org/10.3892/or.2014.3053
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Abstract

It has previously been reported that gene profiles in surgically-resected colorectal cancer tissues are altered over time possibly due to the different tissue-acquisition methods and sample extraction timing that were used. However, the changes that occur are still not clearly understood. In the present study, time-dependent changes in gene expression profiling in colorectal surgical specimens were analyzed. Normal and tumor tissues at several time-points (0, 30, 60 and 120 min) were extracted, and RNA quality, microarray experiments, quantitative PCR and bioinformatics clustering were performed. Although RNA integrity was preserved 2 h after resection, inherent increased/decreased gene expression was observed from 30-120 min in approximately 10% of genes. Bioinformatics clustering could not distinguish case-by-case, probably due to gene profiling changes. Irregular changes in gene expression after surgical resection were found, which could be a crucial confounding factor for quantitative analyses.
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Copy and paste a formatted citation
Spandidos Publications style
Yamagishi A, Matsumoto S, Watanabe A, Mizuguchi Y, Hara K, Kan H, Yamada T, Koizumi M, Shinji S, Matsuda A, Matsuda A, et al: Gene profiling and bioinformatics analyses reveal time course differential gene expression in surgically resected colorectal tissues. Oncol Rep 31: 1531-1538, 2014.
APA
Yamagishi, A., Matsumoto, S., Watanabe, A., Mizuguchi, Y., Hara, K., Kan, H. ... Uchida, E. (2014). Gene profiling and bioinformatics analyses reveal time course differential gene expression in surgically resected colorectal tissues. Oncology Reports, 31, 1531-1538. https://doi.org/10.3892/or.2014.3053
MLA
Yamagishi, A., Matsumoto, S., Watanabe, A., Mizuguchi, Y., Hara, K., Kan, H., Yamada, T., Koizumi, M., Shinji, S., Matsuda, A., Sasaki, J., Shimada, T., Uchida, E."Gene profiling and bioinformatics analyses reveal time course differential gene expression in surgically resected colorectal tissues". Oncology Reports 31.4 (2014): 1531-1538.
Chicago
Yamagishi, A., Matsumoto, S., Watanabe, A., Mizuguchi, Y., Hara, K., Kan, H., Yamada, T., Koizumi, M., Shinji, S., Matsuda, A., Sasaki, J., Shimada, T., Uchida, E."Gene profiling and bioinformatics analyses reveal time course differential gene expression in surgically resected colorectal tissues". Oncology Reports 31, no. 4 (2014): 1531-1538. https://doi.org/10.3892/or.2014.3053
Copy and paste a formatted citation
x
Spandidos Publications style
Yamagishi A, Matsumoto S, Watanabe A, Mizuguchi Y, Hara K, Kan H, Yamada T, Koizumi M, Shinji S, Matsuda A, Matsuda A, et al: Gene profiling and bioinformatics analyses reveal time course differential gene expression in surgically resected colorectal tissues. Oncol Rep 31: 1531-1538, 2014.
APA
Yamagishi, A., Matsumoto, S., Watanabe, A., Mizuguchi, Y., Hara, K., Kan, H. ... Uchida, E. (2014). Gene profiling and bioinformatics analyses reveal time course differential gene expression in surgically resected colorectal tissues. Oncology Reports, 31, 1531-1538. https://doi.org/10.3892/or.2014.3053
MLA
Yamagishi, A., Matsumoto, S., Watanabe, A., Mizuguchi, Y., Hara, K., Kan, H., Yamada, T., Koizumi, M., Shinji, S., Matsuda, A., Sasaki, J., Shimada, T., Uchida, E."Gene profiling and bioinformatics analyses reveal time course differential gene expression in surgically resected colorectal tissues". Oncology Reports 31.4 (2014): 1531-1538.
Chicago
Yamagishi, A., Matsumoto, S., Watanabe, A., Mizuguchi, Y., Hara, K., Kan, H., Yamada, T., Koizumi, M., Shinji, S., Matsuda, A., Sasaki, J., Shimada, T., Uchida, E."Gene profiling and bioinformatics analyses reveal time course differential gene expression in surgically resected colorectal tissues". Oncology Reports 31, no. 4 (2014): 1531-1538. https://doi.org/10.3892/or.2014.3053
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