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Article Open Access

Upregulation of long non-coding RNA urothelial carcinoma associated 1 by CCAAT/enhancer binding protein α contributes to bladder cancer cell growth and reduced apoptosis

  • Authors:
    • Mei Xue
    • Xu Li
    • Wenjing Wu
    • Shuwan Zhang
    • Shouzhen Wu
    • Zhengkun Li
    • Wei Chen
  • View Affiliations / Copyright

    Affiliations: Center for Translational Medicine, The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, P.R. China, Clinical Laboratory, The First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, P.R. China
    Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1993-2000
    |
    Published online on: March 19, 2014
       https://doi.org/10.3892/or.2014.3092
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Abstract

Long non-coding RNA urothelial carcinoma associated 1 (lncRNA-UCA1) is upregulated in bladder cancer and plays a pivotal role in bladder cancer progression and metastasis. Recent studies and our research found that lncRNA-UCA1 may be an important biomarker and therapeutic target for bladder cancer. However, the molecular mechanism involved in the upregulation of lncRNA-UCA1 in bladder cancer is largely unknown. In the present study, we showed that lncRNA-UCA1 expression in bladder cancer cells was upregulated by transcription factor CCAAT/enhancer binding protein α (C/EBPα), which was the only candidate transcription factor simultaneously predicted by a total of five bioinformatical software programs. Electrophoretic mobility shift assay and chromatin immunoprecipitation assay indicated that C/EBPα bound to the lncRNA-UCA1 core promoter region in vitro and in vivo. The luciferase assays further showed that there was a point mutation (A231G) in the C/EBPα binding site of the lncRNA-UCA1 core promoter in various bladder cancer cell lines, which in turn significantly increased the transcriptional activity of lncRNA-UCA1. We also demonstrated that C/EBPα siRNA treatment contributed to the downregulation of lncRNA-UCA1 expression, whereas overexpression of C/EBPα enhanced lncRNA-UCA1 expression. Furthermore, lncRNA-UCA1 transcriptional repression by C/EBPα siRNA sharply reduced cell viability and induced cell apoptosis in vitro. Collectively, our results provide a novel therapeutic strategy for bladder cancer by effectively interrupting the binding of the lncRNA-UCA1 promoter and certain transcription factors, so as to reverse the upregulation of lncRNA-UCA1 and prevent bladder cancer progression.
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Copy and paste a formatted citation
Spandidos Publications style
Xue M, Li X, Wu W, Zhang S, Wu S, Li Z and Chen W: Upregulation of long non-coding RNA urothelial carcinoma associated 1 by CCAAT/enhancer binding protein α contributes to bladder cancer cell growth and reduced apoptosis. Oncol Rep 31: 1993-2000, 2014.
APA
Xue, M., Li, X., Wu, W., Zhang, S., Wu, S., Li, Z., & Chen, W. (2014). Upregulation of long non-coding RNA urothelial carcinoma associated 1 by CCAAT/enhancer binding protein α contributes to bladder cancer cell growth and reduced apoptosis. Oncology Reports, 31, 1993-2000. https://doi.org/10.3892/or.2014.3092
MLA
Xue, M., Li, X., Wu, W., Zhang, S., Wu, S., Li, Z., Chen, W."Upregulation of long non-coding RNA urothelial carcinoma associated 1 by CCAAT/enhancer binding protein α contributes to bladder cancer cell growth and reduced apoptosis". Oncology Reports 31.5 (2014): 1993-2000.
Chicago
Xue, M., Li, X., Wu, W., Zhang, S., Wu, S., Li, Z., Chen, W."Upregulation of long non-coding RNA urothelial carcinoma associated 1 by CCAAT/enhancer binding protein α contributes to bladder cancer cell growth and reduced apoptosis". Oncology Reports 31, no. 5 (2014): 1993-2000. https://doi.org/10.3892/or.2014.3092
Copy and paste a formatted citation
x
Spandidos Publications style
Xue M, Li X, Wu W, Zhang S, Wu S, Li Z and Chen W: Upregulation of long non-coding RNA urothelial carcinoma associated 1 by CCAAT/enhancer binding protein α contributes to bladder cancer cell growth and reduced apoptosis. Oncol Rep 31: 1993-2000, 2014.
APA
Xue, M., Li, X., Wu, W., Zhang, S., Wu, S., Li, Z., & Chen, W. (2014). Upregulation of long non-coding RNA urothelial carcinoma associated 1 by CCAAT/enhancer binding protein α contributes to bladder cancer cell growth and reduced apoptosis. Oncology Reports, 31, 1993-2000. https://doi.org/10.3892/or.2014.3092
MLA
Xue, M., Li, X., Wu, W., Zhang, S., Wu, S., Li, Z., Chen, W."Upregulation of long non-coding RNA urothelial carcinoma associated 1 by CCAAT/enhancer binding protein α contributes to bladder cancer cell growth and reduced apoptosis". Oncology Reports 31.5 (2014): 1993-2000.
Chicago
Xue, M., Li, X., Wu, W., Zhang, S., Wu, S., Li, Z., Chen, W."Upregulation of long non-coding RNA urothelial carcinoma associated 1 by CCAAT/enhancer binding protein α contributes to bladder cancer cell growth and reduced apoptosis". Oncology Reports 31, no. 5 (2014): 1993-2000. https://doi.org/10.3892/or.2014.3092
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