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Article

MCM7 serves as a prognostic marker in diffuse-type gastric adenocarcinoma and siRNA-mediated knockdown suppresses its oncogenic function

  • Authors:
    • Wei Kang
    • Joanna H.M. Tong
    • Anthony W.H. Chan
    • Alfred S.L. Cheng
    • Jun Yu
    • Kafai To
  • View Affiliations / Copyright

    Affiliations: Department of Anatomical and Cellular Pathology, State Key Laboratory in Oncology in South China, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, SAR, P.R. China, School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, SAR, P.R. China, Institute of Digestive Disease, Partner State Key Laboratory of Digestive Disease, Sir Y.K. Pao Cancer Center, The Chinese University of Hong Kong, Hong Kong, SAR, P.R. China
  • Pages: 2071-2078
    |
    Published online on: March 19, 2014
       https://doi.org/10.3892/or.2014.3094
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Abstract

MCM7 (mini-chromosome maintenance protein 7) is essential for the initiation of genomic replication as a component of the pre-replication complex. The present study aimed to analyze its expression, clinical significance and biological functions in gastric adenocarcinoma (GAC). The MCM7 protein was upregulated in all 9 GAC cell lines. In 6 paired primary GACs, MCM7 was upregulated in tumor compared with the corresponding non-tumorous gastric tissues. In normal gastric epithelium tissue, MCM7 was strictly expressed in the proliferative compartment. MCM7 knockdown by siRNA in gastric cancer cell line AGS and NCI-N87 significantly suppressed cell proliferation, inhibited monolayer colony formation, reduced cell invasion and induced late apoptosis. Its nuclear expression correlated with advanced age and poorer disease specific survival in diffuse-type GACs. All the findings supported that MCM7 might play an oncogenic role in gastric tumorigenesis. It serves as a potential prognostic marker and therapeutic target in diffuse-type GACs.
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1 

Kelley JR and Duggan JM: Gastric cancer epidemiology and risk factors. J Clin Epidemiol. 56:1–9. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Smith MG, Hold GL, Tahara E and El-Omar EM: Cellular and molecular aspects of gastric cancer. World J Gastroenterol. 12:2979–2990. 2006.

3 

Shiota S, Suzuki R and Yamaoka Y: The significance of virulence factors in Helicobacter pylori. J Dig Dis. 14:341–349. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Sepulveda AR: Helicobacter, inflammation, and gastric cancer. Curr Pathobiol Rep. 1:9–18. 2013. View Article : Google Scholar

5 

Zhong C, Li KN, Bi JW and Wang BC: Sodium intake, salt taste and gastric cancer risk according to Helicobacter pylori infection, smoking, histological type and tumor site in China. Asian Pac J Cancer Prev. 13:2481–2484. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Zhang MW, Jin MJ, Yu YX, et al: Associations of lifestyle-related factors, hsa-miR-149 and hsa-miR-605 gene polymorphisms with gastrointestinal cancer risk. Mol Carcinog. 51(Suppl 1): E21–E31. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Gomceli I, Demiriz B and Tez M: Gastric carcinogenesis. World J Gastroenterol. 18:5164–5170. 2012.PubMed/NCBI

8 

Green D, Ponce de Leon S, Leon-Rodriguez E and Sosa-Sanchez R: Adenocarcinoma of the stomach: univariate and multivariate analysis of factors associated with survival. Am J Clin Oncol. 25:84–89. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Kebebew E, Peng M, Reiff E, Duh QY, Clark OH and McMillan A: Diagnostic and prognostic value of cell-cycle regulatory genes in malignant thyroid neoplasms. World J Surg. 30:767–774. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Luo JH: Oncogenic activity of MCM7 transforming cluster. World J Clin Oncol. 2:120–124. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Wei Q, Li J, Liu T, Tong X and Ye X: Phosphorylation of minichromosome maintenance protein 7 (MCM7) by cyclin/cyclin-dependent kinase affects its function in cell cycle regulation. J Biol Chem. 288:19715–19725. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Ishimi Y: A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex. J Biol Chem. 272:24508–24513. 1997. View Article : Google Scholar : PubMed/NCBI

13 

You Z, Komamura Y and Ishimi Y: Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity. Mol Cell Biol. 19:8003–8015. 1999.PubMed/NCBI

14 

Honeycutt KA, Chen Z, Koster MI, et al: Deregulated minichromosomal maintenance protein MCM7 contributes to oncogene driven tumorigenesis. Oncogene. 25:4027–4032. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Ren B, Yu G, Tseng GC, et al: MCM7 amplification and overexpression are associated with prostate cancer progression. Oncogene. 25:1090–1098. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Kan T, Sato F, Ito T, et al: The miR-106b-25 polycistron, activated by genomic amplification, functions as an oncogene by suppressing p21 and Bim. Gastroenterology. 136:1689–1700. 2009.

17 

Kang W, Tong JH, Chan AW, et al: Stathmin1 plays oncogenic role and is a target of microRNA-223 in gastric cancer. PLoS One. 7:e339192012. View Article : Google Scholar : PubMed/NCBI

18 

Kang W, Tong JH, Chan AW, et al: Yes-associated protein 1 exhibits oncogenic property in gastric cancer and its nuclear accumulation associates with poor prognosis. Clin Cancer Res. 17:2130–2139. 2011. View Article : Google Scholar : PubMed/NCBI

19 

van Dekken H, Tilanus HW, Hop WC, et al: Array comparative genomic hybridization, expression array, and protein analysis of critical regions on chromosome arms 1q, 7q, and 8p in adenocarcinomas of the gastroesophageal junction. Cancer Genet Cytogenet. 189:37–42. 2009.

20 

Ambs S, Prueitt RL, Yi M, et al: Genomic profiling of microRNA and messenger RNA reveals deregulated microRNA expression in prostate cancer. Cancer Res. 68:6162–6170. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Petrocca F, Visone R, Onelli MR, et al: E2F1-regulated microRNAs impair TGFβ-dependent cell-cycle arrest and apoptosis in gastric cancer. Cancer Cell. 13:272–286. 2008.

22 

Fristrup N, Birkenkamp-Demtroder K, Reinert T, et al: Multicenter validation of cyclin D1, MCM7, TRIM29, and UBE2C as prognostic protein markers in non-muscle-invasive bladder cancer. Am J Pathol. 182:339–349. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Liu YZ, Jiang YY, Hao JJ, et al: Prognostic significance of MCM7 expression in the bronchial brushings of patients with non-small cell lung cancer (NSCLC). Lung Cancer. 77:176–182. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Toyokawa G, Masuda K, Daigo Y, et al: Minichromosome Maintenance Protein 7 is a potential therapeutic target in human cancer and a novel prognostic marker of non-small cell lung cancer. Mol Cancer. 10:652011. View Article : Google Scholar : PubMed/NCBI

25 

Marnerides A, Vassilakopoulos TP, Boltetsou E, et al: Immunohistochemical expression and prognostic significance of CCND3, MCM2 and MCM7 in Hodgkin lymhoma. Anticancer Res. 31:3585–3594. 2011.PubMed/NCBI

26 

Zhou YM, Zhang XF, Cao L, et al: MCM7 expression predicts post-operative prognosis for hepatocellular carcinoma. Liver Int. 32:1505–1509. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Saydam O, Senol O, Schaaij-Visser TB, et al: Comparative protein profiling reveals minichromosome maintenance (MCM) proteins as novel potential tumor markers for meningiomas. J Proteome Res. 9:485–494. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Pillaire MJ, Selves J, Gordien K, et al: A ‘DNA replication’ signature of progression and negative outcome in colorectal cancer. Oncogene. 29:876–887. 2010.

29 

Nishihara K, Shomori K, Fujioka S, et al: Minichromosome maintenance protein 7 in colorectal cancer: implication of prognostic significance. Int J Oncol. 33:245–251. 2008.PubMed/NCBI

30 

Lau KM, Chan QK, Pang JC, et al: Minichromosome maintenance proteins 2, 3 and 7 in medulloblastoma: overexpression and involvement in regulation of cell migration and invasion. Oncogene. 29:5475–5489. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Feng CJ, Li HJ, Li JN, Lu YJ and Liao GQ: Expression of Mcm7 and Cdc6 in oral squamous cell carcinoma and precancerous lesions. Anticancer Res. 28:3763–3769. 2008.PubMed/NCBI

32 

Shi YK, Yu YP, Tseng GC and Luo JH: Inhibition of prostate cancer growth and metastasis using small interference RNA specific for minichromosome complex maintenance component 7. Cancer Gene Ther. 17:694–699. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Fujioka S, Shomori K, Nishihara K, et al: Expression of minichromosome maintenance 7 (MCM7) in small lung adenocarcinomas (pT1): Prognostic implication. Lung Cancer. 65:223–229. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Ishino H, Hara Y, Takekoshi S, et al: Ki-67 and minichromosome maintenance-7 (MCM7) expression in canine pituitary corticotroph adenomas. Domest Anim Endocrinol. 41:207–213. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Li SS, Xue WC, Khoo US, et al: Replicative MCM7 protein as a proliferation marker in endometrial carcinoma: a tissue microarray and clinicopathological analysis. Histopathology. 46:307–313. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kang W, Tong JH, Chan AW, Cheng AS, Yu J and To K: MCM7 serves as a prognostic marker in diffuse-type gastric adenocarcinoma and siRNA-mediated knockdown suppresses its oncogenic function. Oncol Rep 31: 2071-2078, 2014.
APA
Kang, W., Tong, J.H., Chan, A.W., Cheng, A.S., Yu, J., & To, K. (2014). MCM7 serves as a prognostic marker in diffuse-type gastric adenocarcinoma and siRNA-mediated knockdown suppresses its oncogenic function. Oncology Reports, 31, 2071-2078. https://doi.org/10.3892/or.2014.3094
MLA
Kang, W., Tong, J. H., Chan, A. W., Cheng, A. S., Yu, J., To, K."MCM7 serves as a prognostic marker in diffuse-type gastric adenocarcinoma and siRNA-mediated knockdown suppresses its oncogenic function". Oncology Reports 31.5 (2014): 2071-2078.
Chicago
Kang, W., Tong, J. H., Chan, A. W., Cheng, A. S., Yu, J., To, K."MCM7 serves as a prognostic marker in diffuse-type gastric adenocarcinoma and siRNA-mediated knockdown suppresses its oncogenic function". Oncology Reports 31, no. 5 (2014): 2071-2078. https://doi.org/10.3892/or.2014.3094
Copy and paste a formatted citation
x
Spandidos Publications style
Kang W, Tong JH, Chan AW, Cheng AS, Yu J and To K: MCM7 serves as a prognostic marker in diffuse-type gastric adenocarcinoma and siRNA-mediated knockdown suppresses its oncogenic function. Oncol Rep 31: 2071-2078, 2014.
APA
Kang, W., Tong, J.H., Chan, A.W., Cheng, A.S., Yu, J., & To, K. (2014). MCM7 serves as a prognostic marker in diffuse-type gastric adenocarcinoma and siRNA-mediated knockdown suppresses its oncogenic function. Oncology Reports, 31, 2071-2078. https://doi.org/10.3892/or.2014.3094
MLA
Kang, W., Tong, J. H., Chan, A. W., Cheng, A. S., Yu, J., To, K."MCM7 serves as a prognostic marker in diffuse-type gastric adenocarcinoma and siRNA-mediated knockdown suppresses its oncogenic function". Oncology Reports 31.5 (2014): 2071-2078.
Chicago
Kang, W., Tong, J. H., Chan, A. W., Cheng, A. S., Yu, J., To, K."MCM7 serves as a prognostic marker in diffuse-type gastric adenocarcinoma and siRNA-mediated knockdown suppresses its oncogenic function". Oncology Reports 31, no. 5 (2014): 2071-2078. https://doi.org/10.3892/or.2014.3094
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