Suppressive role of miR-502-5p in breast cancer via downregulation of TRAF2

Sun,Li-Li; Wang,Jian; Zhao,Zhi-Juan; Liu,Ning; Wang,Ai-Lian; Ren,Hua-Yan; Yang,Fan; Diao,Ke-Xin; Fu,Wei-Neng; Wan,En-Hua; Mi,Xiao-Yi

doi:10.3892/or.2014.3105

Suppressive role of miR-502-5p in breast cancer via downregulation of TRAF2

Authors:
- Li-Li Sun
- Jian Wang
- Zhi-Juan Zhao
- Ning Liu
- Ai-Lian Wang
- Hua-Yan Ren
- Fan Yang
- Ke-Xin Diao
- Wei-Neng Fu
- En-Hua Wan
- Xiao-Yi Mi
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Affiliations: Department of Pathology, First Affiliated Hospital and College of Basic Medical Science, China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Pancreatic Surgery, First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Medical Genetics, China Medical University, Shenyang, Liaoning 110001, P.R. China
Published online on: March 21, 2014 https://doi.org/10.3892/or.2014.3105
Pages: 2085-2092

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Abstract

TRAF2 promotes cancer cell survival, proliferation and metastasis through the NF-κB pathway by directly interacting with various TNF recepors. However, the molecular mechanism of TRAF2 dysregulation in breast cancer remains to be elucidated. In the present study, miR-502-5p was predicted as a potential regulator of TRAF2. miR-502-5p was significantly downregulated in breast cancer tissues when compared to the level in paired normal breast tissues. The breast cancer cell lines including MCF-7 and MDA-MB-231 expressed a lower level of miR-502-5p when compared to the level in the non-malignant breast epithelial cell line MCF-10A. In vitro, miR-502-5p enhanced early apoptosis and inhibited proliferation of breast cancer cells. Luciferase reporter assay results showed that miR-502-5p could bind to the 3'-untranslated region of the TRAF2 gene, thus, exerting an inhibitory effect on TRAF2. Furthermore, silencing of TRAF2 exhibited effects similar to those of exogenous miR‑502-5p, while overexpression of TRAF2 partially abrogated miR-502-5p-mediated suppression in breast cancer cells. In conclusion, miR-502-5p may act as a tumor-suppressor gene by targeting oncogenic TRAF2 in breast cancer and, therefore, may be a potential diagnostic and anticancer therapeutic marker for breast cancer.

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