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Article

UHRF1 depletion suppresses growth of gallbladder cancer cells through induction of apoptosis and cell cycle arrest

  • Authors:
    • Yiyu Qin
    • Jiandong Wang
    • Wei Gong
    • Mingdi Zhang
    • Zhaohui Tang
    • Jun Zhang
    • Zhiwei Quan
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, P.R. China, Ministry of Education-Shanghai Key Laboratory of Children's Environmental Health, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, P.R. China
  • Pages: 2635-2643
    |
    Published online on: April 23, 2014
       https://doi.org/10.3892/or.2014.3145
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Abstract

Ubiquitin-like containing PHD and RING finger domains 1 (UHRF1), overexpressed in various human malignancies, functions as an important regulator in cell proliferation and epigenetic regulation. Depletion of UHRF1 has shown potential antitumor activities in several types of cancer. However, the role of UHRF1 in gallbladder cancer (GBC) has not been investigated. RT-PCR, western blotting and immunohistochemistry were performed to examine UHRF1 expression at mRNA and protein levels in GBC tissues and cell lines. UHRF1 siRNA and UHRF1 shRNA were used to deplete the expression of UHRF1. The results showed that UHRF1 was overexpressed in GBC and its expression correlated with advanced TNM stage and presence of lymph node metastasis. UHRF1 depletion in GBC-SD and NOZ cells markedly inhibited proliferation, migration in vitro and the ability of these cells to form tumors in vivo. UHRF1 depletion upregulated the expression of PML and triggered extrinsic and intrinsic apoptotic pathways by promoting the expression of FasL/FADD, bax, cytosolic cytochrome c, cleaved caspase-8, -9 and -3 and cleaved PRAP and by suppressing bcl-2 expression in GBC-SD and NOZ cells. In addition, UHRF1 depletion induced cell cycle arrest at G1/S transition by inducing p21 in a p53-independent manner in GBC-SD and NOZ cells. Our findings suggest that UHRF1 is involved in the proliferation and migration of GBC cells and may serve as a biomarker or even a therapeutic target for GBC.
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Copy and paste a formatted citation
Spandidos Publications style
Qin Y, Wang J, Gong W, Zhang M, Tang Z, Zhang J and Quan Z: UHRF1 depletion suppresses growth of gallbladder cancer cells through induction of apoptosis and cell cycle arrest. Oncol Rep 31: 2635-2643, 2014.
APA
Qin, Y., Wang, J., Gong, W., Zhang, M., Tang, Z., Zhang, J., & Quan, Z. (2014). UHRF1 depletion suppresses growth of gallbladder cancer cells through induction of apoptosis and cell cycle arrest. Oncology Reports, 31, 2635-2643. https://doi.org/10.3892/or.2014.3145
MLA
Qin, Y., Wang, J., Gong, W., Zhang, M., Tang, Z., Zhang, J., Quan, Z."UHRF1 depletion suppresses growth of gallbladder cancer cells through induction of apoptosis and cell cycle arrest". Oncology Reports 31.6 (2014): 2635-2643.
Chicago
Qin, Y., Wang, J., Gong, W., Zhang, M., Tang, Z., Zhang, J., Quan, Z."UHRF1 depletion suppresses growth of gallbladder cancer cells through induction of apoptosis and cell cycle arrest". Oncology Reports 31, no. 6 (2014): 2635-2643. https://doi.org/10.3892/or.2014.3145
Copy and paste a formatted citation
x
Spandidos Publications style
Qin Y, Wang J, Gong W, Zhang M, Tang Z, Zhang J and Quan Z: UHRF1 depletion suppresses growth of gallbladder cancer cells through induction of apoptosis and cell cycle arrest. Oncol Rep 31: 2635-2643, 2014.
APA
Qin, Y., Wang, J., Gong, W., Zhang, M., Tang, Z., Zhang, J., & Quan, Z. (2014). UHRF1 depletion suppresses growth of gallbladder cancer cells through induction of apoptosis and cell cycle arrest. Oncology Reports, 31, 2635-2643. https://doi.org/10.3892/or.2014.3145
MLA
Qin, Y., Wang, J., Gong, W., Zhang, M., Tang, Z., Zhang, J., Quan, Z."UHRF1 depletion suppresses growth of gallbladder cancer cells through induction of apoptosis and cell cycle arrest". Oncology Reports 31.6 (2014): 2635-2643.
Chicago
Qin, Y., Wang, J., Gong, W., Zhang, M., Tang, Z., Zhang, J., Quan, Z."UHRF1 depletion suppresses growth of gallbladder cancer cells through induction of apoptosis and cell cycle arrest". Oncology Reports 31, no. 6 (2014): 2635-2643. https://doi.org/10.3892/or.2014.3145
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