miR-101, downregulated in retinoblastoma, functions as a tumor suppressor in human retinoblastoma cells by targeting EZH2

Lei,Qiong; Shen,Fengmei; Wu,Jie; Zhang,Weishan; Wang,Jiahui; Zhang,Lin

doi:10.3892/or.2014.3167

miR-101, downregulated in retinoblastoma, functions as a tumor suppressor in human retinoblastoma cells by targeting EZH2

Authors:
- Qiong Lei
- Fengmei Shen
- Jie Wu
- Weishan Zhang
- Jiahui Wang
- Lin Zhang
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Affiliations: Department of Ophthalmology, First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710062, P.R. China, Department of Ophthalmology, No.1 Hospital of Xi'an, Xi'an, Shaanxi 710062, P.R. China
Published online on: May 7, 2014 https://doi.org/10.3892/or.2014.3167
Pages: 261-269

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Abstract

Accumulating evidence indicates that microRNAs are involved in multiple processes in cancer development and progression, and several miRNAs have emerged as candidate components of oncogene or tumor-suppressor networks in retinoblastoma. miR-101 has been identified as a tumor suppressor in several types of human cancer. However, the specific function of miR-101 in retinoblastoma remains unclear. In the present study, we found that the expression of miR-101 in retinoblastoma tissues was much lower than that in the normal controls. In addition, downregulation of miR-101 more frequently occurred in retinoblastoma specimens with adverse clinicopathological and histopathological features. In addition, miR-101 inhibited cell viability and progression in retinoblastoma cells by promoting cell apoptosis and arresting the cell cycle. Finally, we found that miR-101 directly inhibited EZH2 expression by targeting its 3'-UTR, and EZH2 was upregulated and inversely correlated with miR-101 expression in the retinoblastoma tissues. Thus, for the first time, we provide convincing evidence that downregulation of miR-101 is associated with tumor aggressiveness in retinoblastoma and inhibits cell growth and proliferation of retinoblastoma cells by targeting EZH2. In conclusion, all the evidence supports the tumor-suppressor role of miR-101 in human retinoblastoma.

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