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July-2014 Volume 32 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Article

β1 integrin modulates tumor growth and apoptosis of human colorectal cancer

  • Authors:
    • Jia Song
    • Jixiang Zhang
    • Jing Wang
    • Zhuo Cao
    • Jun Wang
    • Xufeng Guo
    • Weiguo Dong
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
  • Pages: 302-308
    |
    Published online on: May 7, 2014
       https://doi.org/10.3892/or.2014.3168
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Abstract

We aimed to ascertain whether β1 integrin (ITGB1) induces apoptosis of colorectal cancer (CRC) through regulation of the mitochondrial pathway in vitro and in vivo. We generated lentiviral vectors expressing ITGB1 or ITGB1-specific RNAi and an unrelated control vector. After infection of the HT29 cells, we used western blot analysis and flow cytometric analysis to validate the patterns of ITGB1 expression. Proliferation and apoptosis were evaluated by colony formation assay, flow cytometry and western blot analysis. Upregulation of ITGB1 significantly increased the proliferation of HT29 cells; however, the levels of Bcl-2 and cyclin D1 proteins were upregulated while Bax, caspase-3, caspase-9 and p21 were downregulated in the HT29-ITGB1 cells compared to these levels in the controls. Hoechst 33258 staining and flow cytometric analysis showed that ITGB1 may play a significant role in the apoptosis of CRC cells. Moreover, ITGB1 promoted the proliferation of cells in a xenograft tumor mouse model. TUNEL staining revealed a marked increase in the percentage of positive cells in the HT29-RNAi group (84.3±4.0%), which were more significant than in the HT29-ITGB1 group (48.3±2.9%) and the other two control groups, HT29 (52.0±3.6%) and HT29-NC (49.7±4.5%). These results suggest that ITGB1 regulates the growth and apoptosis of human colorectal cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Song J, Zhang J, Wang J, Cao Z, Wang J, Guo X and Dong W: β1 integrin modulates tumor growth and apoptosis of human colorectal cancer. Oncol Rep 32: 302-308, 2014.
APA
Song, J., Zhang, J., Wang, J., Cao, Z., Wang, J., Guo, X., & Dong, W. (2014). β1 integrin modulates tumor growth and apoptosis of human colorectal cancer. Oncology Reports, 32, 302-308. https://doi.org/10.3892/or.2014.3168
MLA
Song, J., Zhang, J., Wang, J., Cao, Z., Wang, J., Guo, X., Dong, W."β1 integrin modulates tumor growth and apoptosis of human colorectal cancer". Oncology Reports 32.1 (2014): 302-308.
Chicago
Song, J., Zhang, J., Wang, J., Cao, Z., Wang, J., Guo, X., Dong, W."β1 integrin modulates tumor growth and apoptosis of human colorectal cancer". Oncology Reports 32, no. 1 (2014): 302-308. https://doi.org/10.3892/or.2014.3168
Copy and paste a formatted citation
x
Spandidos Publications style
Song J, Zhang J, Wang J, Cao Z, Wang J, Guo X and Dong W: β1 integrin modulates tumor growth and apoptosis of human colorectal cancer. Oncol Rep 32: 302-308, 2014.
APA
Song, J., Zhang, J., Wang, J., Cao, Z., Wang, J., Guo, X., & Dong, W. (2014). β1 integrin modulates tumor growth and apoptosis of human colorectal cancer. Oncology Reports, 32, 302-308. https://doi.org/10.3892/or.2014.3168
MLA
Song, J., Zhang, J., Wang, J., Cao, Z., Wang, J., Guo, X., Dong, W."β1 integrin modulates tumor growth and apoptosis of human colorectal cancer". Oncology Reports 32.1 (2014): 302-308.
Chicago
Song, J., Zhang, J., Wang, J., Cao, Z., Wang, J., Guo, X., Dong, W."β1 integrin modulates tumor growth and apoptosis of human colorectal cancer". Oncology Reports 32, no. 1 (2014): 302-308. https://doi.org/10.3892/or.2014.3168
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