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Role of c-Src activity in the regulation of gastric cancer cell migration

  • Authors:
    • Yun Yang
    • Zhi-Gang Bai
    • Jie Yin
    • Guo-Cong Wu
    • Zhong-Tao Zhang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Xuanwu, Beijing 100050, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 45-49
    |
    Published online on: May 15, 2014
       https://doi.org/10.3892/or.2014.3188
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Abstract

Gastric cancer is associated with increased migration and invasion. In the present study, we explored the role of c-Src in gastric cancer cell migration and invasion. BGC-823 gastric cancer cells were used to investigate migration following treatment of these cells with the c-Src inhibitors, PP2 and SU6656. Migration and invasion were analyzed by wound healing and Transwell assays. Western blot analysis was used to detect the expression of MT1-MMP and VEGF-C, while the activity of MMP2 and MMP9 was monitored with gelatin zymography assay. Immunoprecipitation was used to detect interactions among furin, pro-MT1-MMP and pro-VEGF-C. MT1-MMP and VEGF-C expression levels were inhibited by PP2 and SU6656 treatment, in accordance with decreased c-Src activity. Similarly, the zymography assay demonstrated that the activity of MMP2 and MMP9 was decreased following PP2 or SU6656 treatment. Blockade of c-Src also inhibited the invasive and migratory capacity of BGC-823 cells. Notably, c-Src interacted with furin in vivo, while interactions between furin and its substrates, pro-MT1-MMP and pro-VEGF-C, were decreased by c-Src inhibitors. In conclusion, the interaction among furin and pro-MT1-MMP or pro-VEGF-C or other tumor-associated precursor enzymes can be regulated by c-Src activity, thus reducing or changing the expression of these enzymes in order to reduce the development of gastric cancer, invasion and metastasis.
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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Bai Z, Yin J, Wu G and Zhang Z: Role of c-Src activity in the regulation of gastric cancer cell migration. Oncol Rep 32: 45-49, 2014.
APA
Yang, Y., Bai, Z., Yin, J., Wu, G., & Zhang, Z. (2014). Role of c-Src activity in the regulation of gastric cancer cell migration. Oncology Reports, 32, 45-49. https://doi.org/10.3892/or.2014.3188
MLA
Yang, Y., Bai, Z., Yin, J., Wu, G., Zhang, Z."Role of c-Src activity in the regulation of gastric cancer cell migration". Oncology Reports 32.1 (2014): 45-49.
Chicago
Yang, Y., Bai, Z., Yin, J., Wu, G., Zhang, Z."Role of c-Src activity in the regulation of gastric cancer cell migration". Oncology Reports 32, no. 1 (2014): 45-49. https://doi.org/10.3892/or.2014.3188
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Bai Z, Yin J, Wu G and Zhang Z: Role of c-Src activity in the regulation of gastric cancer cell migration. Oncol Rep 32: 45-49, 2014.
APA
Yang, Y., Bai, Z., Yin, J., Wu, G., & Zhang, Z. (2014). Role of c-Src activity in the regulation of gastric cancer cell migration. Oncology Reports, 32, 45-49. https://doi.org/10.3892/or.2014.3188
MLA
Yang, Y., Bai, Z., Yin, J., Wu, G., Zhang, Z."Role of c-Src activity in the regulation of gastric cancer cell migration". Oncology Reports 32.1 (2014): 45-49.
Chicago
Yang, Y., Bai, Z., Yin, J., Wu, G., Zhang, Z."Role of c-Src activity in the regulation of gastric cancer cell migration". Oncology Reports 32, no. 1 (2014): 45-49. https://doi.org/10.3892/or.2014.3188
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