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Article

Overexpression of flotillin-1 is involved in proliferation and recurrence of bladder transitional cell carcinoma

  • Authors:
    • Yawei Guan
    • Haiyan Song
    • Guohui Zhang
    • Xing Ai
  • View Affiliations / Copyright

    Affiliations: Department of Urinary Surgery, Beijing Military Region General Hospital, Beijing 100700, P.R. China, Department of Blood Transfusion, The 309th Hospital of Chinese PLA, Beijing 100091, P.R. China
  • Pages: 748-754
    |
    Published online on: May 29, 2014
       https://doi.org/10.3892/or.2014.3221
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Abstract

Flotillin-1 (FLOT1) is known to have a role in tumorigenesis; however, the effect of FLOT1 on proliferation and recurrence of human transitional cell carcinoma (TCC) is unclear. Samples from 156 TCC patients and 142 patients undergoing open bladder surgery for indications other than TCC were used in the present study. FLOT1 protein expression was determined by immunohistochemistry and western blot analysis, and mRNA expression was detected by RT-PCR and real-time PCR. A FLOT1-expressing pcDEF3 vector was stably transfected into 4 TCC cell lines and FLOT1 expression was decreased by RNAi. Proliferative analysis of TCC cells was detected by the WST-1 assay and a xenograft model using BALB/C nude mice. The association between FLOT1 expression and TCC recurrence was also analyzed by adhesion, migration and invasion assays. FLOT1 expression in TCC was significantly overexpressed compared to normal urothelial tissue, and the level of FLOT1 expression was significantly correlated with tumor size, pathologic grade, clinical stage and recurrence. In addition, FLOT1 significantly increased the proliferative ability of TCC cells in vitro and in vivo. TCC cells with a high level of FLOT1 expression exhibited a higher level of adhesion, migration and invasion. FLOT1 expression was shown to be upregulated in human TCC. These findings suggest that FLOT1 plays an important role in the proliferation and recurrence of TCC and that silencing FLOT1 expression might be a novel therapeutic strategy.
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Copy and paste a formatted citation
Spandidos Publications style
Guan Y, Song H, Zhang G and Ai X: Overexpression of flotillin-1 is involved in proliferation and recurrence of bladder transitional cell carcinoma. Oncol Rep 32: 748-754, 2014.
APA
Guan, Y., Song, H., Zhang, G., & Ai, X. (2014). Overexpression of flotillin-1 is involved in proliferation and recurrence of bladder transitional cell carcinoma. Oncology Reports, 32, 748-754. https://doi.org/10.3892/or.2014.3221
MLA
Guan, Y., Song, H., Zhang, G., Ai, X."Overexpression of flotillin-1 is involved in proliferation and recurrence of bladder transitional cell carcinoma". Oncology Reports 32.2 (2014): 748-754.
Chicago
Guan, Y., Song, H., Zhang, G., Ai, X."Overexpression of flotillin-1 is involved in proliferation and recurrence of bladder transitional cell carcinoma". Oncology Reports 32, no. 2 (2014): 748-754. https://doi.org/10.3892/or.2014.3221
Copy and paste a formatted citation
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Spandidos Publications style
Guan Y, Song H, Zhang G and Ai X: Overexpression of flotillin-1 is involved in proliferation and recurrence of bladder transitional cell carcinoma. Oncol Rep 32: 748-754, 2014.
APA
Guan, Y., Song, H., Zhang, G., & Ai, X. (2014). Overexpression of flotillin-1 is involved in proliferation and recurrence of bladder transitional cell carcinoma. Oncology Reports, 32, 748-754. https://doi.org/10.3892/or.2014.3221
MLA
Guan, Y., Song, H., Zhang, G., Ai, X."Overexpression of flotillin-1 is involved in proliferation and recurrence of bladder transitional cell carcinoma". Oncology Reports 32.2 (2014): 748-754.
Chicago
Guan, Y., Song, H., Zhang, G., Ai, X."Overexpression of flotillin-1 is involved in proliferation and recurrence of bladder transitional cell carcinoma". Oncology Reports 32, no. 2 (2014): 748-754. https://doi.org/10.3892/or.2014.3221
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