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Article Open Access

Vitamin D analogs combined with 5-fluorouracil in human HT-29 colon cancer treatment

  • Authors:
    • Magdalena Milczarek
    • Beata Filip-Psurska
    • Wiesław Świętnicki
    • Andrzej Kutner
    • Joanna Wietrzyk
  • View Affiliations / Copyright

    Affiliations: Department of Experimental Oncology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland, Wroclaw Research Centre EIT+, 54-066 Wroclaw, Poland, Pharmaceutical Research Institute, 01-793 Warsaw, Poland
    Copyright: © Milczarek et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 491-504
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    Published online on: June 11, 2014
       https://doi.org/10.3892/or.2014.3247
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Abstract

In the present study, we evaluated the antitumor effect of two synthetic analogs of vitamin D, namely PRI-2191 [(24R)-1,24-dihydroxyvitamin D3] and PRI-2205 (5,6-trans calcipotriol), in combined human colon HT-29 cancer treatment with 5-fluorouracil (5-FU). Mice bearing HT-29 tumors transplanted subcutaneously or orthotopically were injected with vitamin D analogs and 5-FU in various schedules. A statistically significant inhibition of subcutaneous or orthotopic tumor growth was observed as a result of combined therapy. In HT-29 tumors and in cells from in vitro culture, we observed increased vitamin D receptor (VDR) expression after treatment with either PRI-2205 or 5-FU alone, or in combination. Moreover, PRI-2205 decreased the percentage of cells from intestinal tumors in G2/M and S stages and increased sub-G1. Increased VDR expression was also observed after combined treatment of mice with 5-FU and PRI-2191. Moreover, our docking studies showed that PRI-2205 has stronger affinity for VDR, DBP and CAR/RXR ligand binding domains than PRI-2191. PRI-2191 analog, used with 5-FU, increased the percentage of subcutaneous tumor cells in G0/G1 and decreased the percentage in G2/M, S and sub-G1 populations as compared to 5-FU alone. In in vitro studies, we observed increased expression of p21 and p-ERK1/2 diminution via use of both analogs as compared to use of 5-FU alone. Simultaneously, PRI-2191 antagonizes some pro-apoptotic activities of 5-FU in vitro. However, in spite of these disadvantageous effects in terms of apoptosis, the therapeutic effect expressed as tumor growth retardation by PRI-2191 is significant. Our results suggest that the mechanism of potentiation of 5-FU antitumor action by both analogs is realized via increased p21 expression and decreased p-ERK1/2 level which may lead to diminution of thymidylate synthase expression. Higher binding affinity for VDR, DBP, but also for CAR\RXR ligand binding domain of PRI-2205 may, in part, explain its very low toxicity with sustained anticancer activity.
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1 

Lamprecht SA and Lipkin M: Chemoprevention of colon cancer by calcium, vitamin D and folate: molecular mechanisms. Nat Rev Cancer. 3:601–614. 2003. View Article : Google Scholar : PubMed/NCBI

2 

McCarthy TC, Li X and Sinal CJ: Vitamin D receptor-dependent regulation of colon multidrug resistance-associated protein 3 gene expression by bile acids. J Biol Chem. 280:23232–23242. 2005. View Article : Google Scholar

3 

Pritchard RS, Baron JA and Gerhardsson de Verdier M: Dietary calcium, vitamin D, and the risk of colorectal cancer in Stockholm, Sweden. Cancer Epidemiol Biomarkers Prev. 5:897–900. 1996.PubMed/NCBI

4 

Terry P, Baron JA, Bergkvist L, Holmberg L and Wolk A: Dietary calcium and vitamin D intake and risk of colorectal cancer: a prospective cohort study in women. Nutr Cancer. 43:39–46. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Berkovich L, Sintov AC and Ben-Shabat S: Inhibition of cancer growth and induction of apoptosis by BGP-13 and BGP-15, new calcipotriene-derived vitamin D3analogs, in-vitro and in-vivo studies. Invest New Drugs. 31:247–255. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Grau MV, Baron JA, Sandler RS, Haile RW, Beach ML, Church TR and Heber D: Vitamin D, calcium supplementation, and colorectal adenomas: results of a randomized trial. J Natl Cancer Inst. 95:1765–1771. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Hartman TJ, Albert PS, Snyder K, Slattery ML, Caan B, Paskett E, Iber F, Kikendall JW, Marshall J, Shike M, Weissfeld J, Brewer B, Schatzkin A and Lanza E: The association of calcium and vitamin D with risk of colorectal adenomas. J Nutr. 135:252–259. 2005.PubMed/NCBI

8 

Baniahmad A and Tsai MJ: Mechanisms of transcriptional activation by steroid hormone receptors. J Cell Biochem. 51:151–156. 1993. View Article : Google Scholar : PubMed/NCBI

9 

Nagpal S, Na S and Rathnachalam R: Noncalcemic actions of vitamin D receptor ligands. Endocr Rev. 26:662–687. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Saramaki A, Banwell CM, Campbell MJ and Carlberg C: Regulation of the human p21 (waf1/cip1) gene promoter via multiple binding sites for p53 and the vitamin D3receptor. Nucleic Acids Res. 34:543–554. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Alvarez-Diaz S, Valle N, Ferrer-Mayorga G, Lombardia L, Herrera M, Dominguez O, Segura MF, Bonilla F, Hernando E and Munoz A: MicroRNA-22 is induced by vitamin D and contributes to its antiproliferative, antimigratory and gene regulatory effects in colon cancer cells. Hum Mol Genet. 21:2157–2165. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Matusiak D, Murillo G, Carroll RE, Mehta RG and Benya RV: Expression of vitamin D receptor and 25-hydroxyvitamin D3-1{alpha}-hydroxylase in normal and malignant human colon. Cancer Epidemiol Biomarkers Prev. 14:2370–2376. 2005.

13 

Tangpricha V, Spina C, Yao M, Chen TC, Wolfe MM and Holick MF: Vitamin D deficiency enhances the growth of MC-26 colon cancer xenografts in Balb/c mice. J Nutr. 135:2350–2354. 2005.PubMed/NCBI

14 

Mokady E, Schwartz B, Shany S and Lamprecht SA: A protective role of dietary vitamin D3in rat colon carcinogenesis. Nutr Cancer. 38:65–73. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Hummel DM, Thiem U, Hobaus J, Mesteri I, Gober L, Stremnitzer C, Graca J, Obermayer-Pietsch B and Kallay E: Prevention of preneoplastic lesions by dietary vitamin D in a mouse model of colorectal carcinogenesis. J Steroid Biochem Mol Biol. 136:284–288. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Larriba MJ, Ordonez-Moran P, Chicote I, Martin-Fernandez G, Puig I, Munoz A and Palmer HG: Vitamin D receptor deficiency enhances Wnt/β-catenin signaling and tumor burden in colon cancer. PLoS ONE. 6:e235242011.PubMed/NCBI

17 

Cho YL, Christensen C, Saunders DE, Lawrence WD, Deppe G, Malviya VK and Malone JM: Combined effects of 1,25-dihydroxyvitamin D3 and platinum drugs on the growth of MCF-7 cells. Cancer Res. 51:2848–2853. 1991.PubMed/NCBI

18 

Ravid A, Rocker D, Machlenkin A, Rotem C, Hochman A, Kessler-Icekson G, Liberman UA and Koren R: 1,25-Dihydroxyvitamin D3 enhances the susceptibility of breast cancer cells to doxorubicin-induced oxidative damage. Cancer Res. 59:862–867. 1999.PubMed/NCBI

19 

Siwinska A, Opolski A, Chrobak A, Wietrzyk J, Wojdat E, Kutner A, Szelejewski W and Radzikowski C: Potentiation of the antiproliferative effect in vitro of doxorubicin, cisplatin and genistein by new analogues of vitamin D. Anticancer Res. 21:1925–1929. 2001.PubMed/NCBI

20 

Pelczynska M, Switalska M, Maciejewska M, Jaroszewicz I, Kutner A and Opolski A: Antiproliferative activity of vitamin D compounds in combination with cytostatics. Anticancer Res. 26:2701–2705. 2006.PubMed/NCBI

21 

Opolski A, Wietrzyk J, Siwinska A, Marcinkowska E, Chrobak A, Radzikowski C and Kutner A: Biological activity in vitro of side-chain modified analogues of calcitriol. Curr Pharm Des. 6:755–765. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Kota BP, Allen JD and Roufogalis BD: The effect of vitamin D3and ketoconazole combination on VDR-mediated P-gp expression and function in human colon adenocarcinoma cells: implications in drug disposition and resistance. Basic Clin Pharmacol Toxicol. 109:97–102. 2011.

23 

Abe J, Nakano T, Nishii Y, Matsumoto T, Ogata E and Ikeda K: A novel vitamin D3analog, 22-oxa-1,25-dihydroxyvitamin D3, inhibits the growth of human breast cancer in vitro and in vivo without causing hypercalcemia. Endocrinology. 129:832–837. 1991.

24 

Abe-Hashimoto J, Kikuchi T, Matsumoto T, Nishii Y, Ogata E and Ikeda K: Antitumor effect of 22-oxa-calcitriol, a noncalcemic analogue of calcitriol, in athymic mice implanted with human breast carcinoma and its synergism with tamoxifen. Cancer Res. 53:2534–2537. 1993.PubMed/NCBI

25 

Chodyński M, Wietrzyk J, Marcinkowska E, Opolski A, Szelejewski W and Kutner A: Synthesis and antiproliferative activity of side-chain unsaturated and homologated analogs of 1,25-dihydroxyvitamin D(2). (24E)-(1S)-24-Dehydro-24a-homo-1,25-dihydroxyergocalciferol and congeners. Steroids. 67:789–798. 2002.PubMed/NCBI

26 

Wietrzyk J, Chodynski M, Fitak H, Wojdat E, Kutner A and Opolski A: Antitumor properties of diastereomeric and geometric analogs of vitamin D3. Anticancer Drugs. 18:447–457. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Wietrzyk J, Nevozhay D, Filip B, Milczarek M and Kutner A: The antitumor effect of lowered doses of cytostatics combined with new analogs of vitamin D in mice. Anticancer Res. 27:3387–3398. 2007.PubMed/NCBI

28 

Wietrzyk J, Pelczynska M, Madej J, Dzimira S, Kusnierczyk H, Kutner A, Szelejewski W and Opolski A: Toxicity and antineoplastic effect of (24R)-1,24-dihydroxyvitamin D3 (PRI-2191). Steroids. 69:629–635. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Wietrzyk J, Nevozhay D, Milczarek M, Filip B and Kutner A: Toxicity and antitumor activity of the vitamin D analogs PRI-1906 and PRI-1907 in combined treatment with cyclophosphamide in a mouse mammary cancer model. Cancer Chemother Pharmacol. 62:787–797. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Milczarek M, Rosinska S, Psurski M, Maciejewska M, Kutner A and Wietrzyk J: Combined colonic cancer treatment with vitamin D analogs and irinotecan or oxaliplatin. Anticancer Res. 33:433–444. 2013.PubMed/NCBI

31 

Lowe SW, Bodis S, McClatchey A, Remington L, Ruley HE, Fisher DE, Housman DE and Jacks T: p53 status and the efficacy of cancer therapy in vivo. Science. 266:807–810. 1994. View Article : Google Scholar : PubMed/NCBI

32 

Sun XX, Dai MS and Lu H: 5-fluorouracil activation of p53 involves an MDM2-ribosomal protein interaction. J Biol Chem. 282:8052–8059. 2007. View Article : Google Scholar : PubMed/NCBI

33 

Chakrabarty S, Radjendirane V, Appelman H and Varani J: Extracellular calcium and calcium sensing receptor function in human colon carcinomas: promotion of E-cadherin expression and suppression of beta-catenin/TCF activation. Cancer Res. 63:67–71. 2003.

34 

Bhagavathula N, Hanosh AW, Nerusu KC, Appelman H, Chakrabarty S and Varani J: Regulation of E-cadherin and beta-catenin by Ca2+in colon carcinoma is dependent on calcium-sensing receptor expression and function. Int J Cancer. 121:1455–1462. 2007.PubMed/NCBI

35 

Chakrabarty S, Wang H, Canaff L, Hendy GN, Appelman H and Varani J: Calcium sensing receptor in human colon carcinoma: interaction with Ca2+and 1,25-dihydroxyvitamin D3. Cancer Res. 65:493–498. 2005.PubMed/NCBI

36 

Wang X, Chen W, Singh N, Promkan M and Liu G: Effects of potential calcium sensing receptor inducers on promoting chemosensitivity of human colon carcinoma cells. Int J Oncol. 36:1573–1580. 2010.PubMed/NCBI

37 

Liu G, Hu X and Chakrabarty S: Vitamin D mediates its action in human colon carcinoma cells in a calcium-sensing receptor-dependent manner: downregulates malignant cell behavior and the expression of thymidylate synthase and survivin and promotes cellular sensitivity to 5-FU. Int J Cancer. 126:631–639. 2010. View Article : Google Scholar

38 

Peters GJ, van der Wilt CL, van Moorsel CJ, Kroep JR, Bergman AM and Ackland SP: Basis for effective combination cancer chemotherapy with antimetabolites. Pharmacol Ther. 87:227–253. 2000. View Article : Google Scholar : PubMed/NCBI

39 

Kim R, Emi M, Tanabe K, Uchida Y and Arihiro K: The role of apoptotic or nonapoptotic cell death in determining cellular response to anticancer treatment. Eur J Surg Oncol. 32:269–277. 2006. View Article : Google Scholar : PubMed/NCBI

40 

Sastry GM, Adzhigirey M, Day T, Annabhimoju R and Sherman W: Protein and ligand preparation: parameters, protocols, and influence on virtual screening enrichments. J Comput Aided Mol Des. 27:221–234. 2013. View Article : Google Scholar : PubMed/NCBI

41 

Friesner RA, Murphy RB, Repasky MP, Frye LL, Greenwood JR, Halgren TA, Sanschagrin PC and Mainz DT: Extra precision glide: docking and scoring incorporating a model of hydrophobic enclosure for protein-ligand complexes. J Med Chem. 49:6177–6196. 2006. View Article : Google Scholar : PubMed/NCBI

42 

Halgren TA, Murphy RB, Friesner RA, Beard HS, Frye LL, Pollard WT and Banks JL: Glide: a new approach for rapid, accurate docking and scoring. 2. Enrichment factors in database screening. J Med Chem. 47:1750–1759. 2004. View Article : Google Scholar : PubMed/NCBI

43 

Friesner RA, Banks JL, Murphy RB, Halgren TA, Klicic JJ, Mainz DT, Repasky MP, Knoll EH, Shelley M, Perry JK, Shaw DE, Francis P and Shenkin PS: Glide: a new approach for rapid, accurate docking and scoring. 1. Method and assessment of docking accuracy. J Med Chem. 47:1739–1749. 2004. View Article : Google Scholar : PubMed/NCBI

44 

Li J, Abel R, Zhu K, Cao Y, Zhao S and Friesner RA: The VSGB 2.0 model: a next generation energy model for high resolution protein structure modeling. Proteins. 79:2794–2812. 2011. View Article : Google Scholar : PubMed/NCBI

45 

Hou T, Wang J, Li Y and Wang W: Assessing the performance of the MM/PBSA and MM/GBSA methods. 1. The accuracy of binding free energy calculations based on molecular dynamics simulations. J Chem Inf Model. 51:69–82. 2011. View Article : Google Scholar : PubMed/NCBI

46 

Hou T, Wang J, Li Y and Wang W: Assessing the performance of the molecular mechanics/Poisson Boltzmann surface area and molecular mechanics/generalized Born surface area methods. II. The accuracy of ranking poses generated from docking. J Comput Chem. 32:866–877. 2011. View Article : Google Scholar : PubMed/NCBI

47 

Rastelli G, Del Rio A, Degliesposti G and Sgobba M: Fast and accurate predictions of binding free energies using MM-PBSA and MM-GBSA. J Comput Chem. 31:797–810. 2010.PubMed/NCBI

48 

Danilenko M and Studzinski GP: Enhancement by other compounds of the anti-cancer activity of vitamin D3and its analogs. Exp Cell Res. 298:339–358. 2004. View Article : Google Scholar : PubMed/NCBI

49 

Milczarek M, Psurski M, Kutner A and Wietrzyk J: Vitamin D analogs enhance the anticancer activity of 5-fluorouracil in an in vivo mouse colon cancer model. BMC Cancer. 13:2942013. View Article : Google Scholar : PubMed/NCBI

50 

Zhao X and Feldman D: Regulation of vitamin D receptor abundance and responsiveness during differentiation of HT-29 human colon cancer cells. Endocrinology. 132:1808–1814. 1993.PubMed/NCBI

51 

Gaschott T, Werz O, Steinmeyer A, Steinhilber D and Stein J: Butyrate-induced differentiation of Caco-2 cells is mediated by vitamin D receptor. Biochem Biophys Res Commun. 288:690–696. 2001. View Article : Google Scholar : PubMed/NCBI

52 

Palmer HG, Gonzalez-Sancho JM, Espada J, Berciano MT, Puig I, Baulida J, Quintanilla M, Cano A, de Herreros AG, Lafarga M and Munoz A: Vitamin D(3) promotes the differentiation of colon carcinoma cells by the induction of E-cadherin and the inhibition of beta-catenin signaling. J Cell Biol. 154:369–387. 2001. View Article : Google Scholar : PubMed/NCBI

53 

Wietrzyk J, Opolski A, Madej J and Radzikowski C: Antitumour and antimetastatic effect of genistein alone or combined with cyclophosphamide in mice transplanted with various tumours depends on the route of tumour transplantation. In Vivo. 14:357–362. 2000.

54 

Hidalgo AA, Paredes R, Garcia VM, Flynn G, Johnson CS, Trump DL and Onate SA: Altered VDR-mediated transcriptional activity in prostate cancer stroma. J Steroid Biochem Mol Biol. 103:731–736. 2007. View Article : Google Scholar : PubMed/NCBI

55 

Spina CS, Tangpricha V, Uskokovic M, Adorinic L, Maehr H and Holick MF: Vitamin D and cancer. Anticancer Res. 26:2515–2524. 2006.

56 

Tocchini-Valentini G, Rochel N, Wurtz JM and Moras D: Crystal structures of the vitamin D nuclear receptor liganded with the vitamin D side chain analogues calcipotriol and secocalcitol, receptor agonists of clinical importance. Insights into a structural basis for the switching of calcipotriol to a receptor antagonist by further side chain modification. J Med Chem. 47:1956–1961. 2004.

57 

Matsunaga T, Yamamoto M, Mimura H, Ohta T, Kiyoki M, Ohba T, Naruchi T, Hosoi J and Kuroki T: 1,24(R)-dihydroxyvitamin D3, a novel active form of vitamin D3 with high activity for inducing epidermal differentiation but decreased hypercalcemic activity. J Dermatol. 17:135–142. 1990.

58 

Bouillon R, Allewaert K, Xiang DZ, Tan BK and van Baelen HJ: Vitamin D analogs with low affinity for the vitamin D binding protein: enhanced in vitro and decreased in vivo activity. J Bone Miner Res. 6:1051–1057. 1991. View Article : Google Scholar : PubMed/NCBI

59 

Xu RX, Lambert MH, Wisely BB, et al: A structural basis for constitutive activity in the human CAR/RXRalpha heterodimer. Mol Cell. 16:919–928. 2004. View Article : Google Scholar : PubMed/NCBI

60 

Wu B, Li S and Dong D: 3D structures and ligand specificities of nuclear xenobiotic receptors CAR, PXR and VDR. Drug Discov Today. 18:574–581. 2013. View Article : Google Scholar : PubMed/NCBI

61 

Sutton AL and MacDonald PN: Vitamin D: more than a ‘bone-a-fide’ hormone. Mol Endocrinol. 17:777–791. 2003.

62 

Moreau A, Maurel P, Vilarem MJ and Pascussi JM: Constitutive androstane receptor-vitamin D receptor crosstalk: consequence on CYP24 gene expression. Biochem Biophys Res Commun. 360:76–82. 2007. View Article : Google Scholar : PubMed/NCBI

63 

Cheng HT, Chen JY, Huang YC, Chang HC and Hung WC: Functional role of VDR in the activation of p27Kip1 by the VDR/Sp1 complex. J Cell Biochem. 98:1450–1456. 2006. View Article : Google Scholar : PubMed/NCBI

64 

Huang YC, Chen JY and Hung WC: Vitamin D3 receptor/Sp1 complex is required for the induction of p27Kip1 expression by vitamin D3. Oncogene. 23:4856–4861. 2004. View Article : Google Scholar : PubMed/NCBI

65 

Diaz GD, Paraskeva C, Thomas MG, Binderup L and Hague A: Apoptosis is induced by the active metabolite of vitamin D3 and its analogue EB1089 in colorectal adenoma and carcinoma cells: possible implications for prevention and therapy. Cancer Res. 60:2304–2312. 2000.PubMed/NCBI

66 

Koren R, Wacksberg S, Weitsman GE and Ravid A: Calcitriol sensitizes colon cancer cells to H2O2-induced cytotoxicity while inhibiting caspase activation. J Steroid Biochem Mol Biol. 101:151–160. 2006. View Article : Google Scholar : PubMed/NCBI

67 

Meng J, Zhang HH, Zhou CX, Li C, Zhang F and Mei QB: The histone deacetylase inhibitor trichostatin A induces cell cycle arrest and apoptosis in colorectal cancer cells via p53-dependent and -independent pathways. Oncol Rep. 28:384–388. 2012.PubMed/NCBI

68 

Stambolsky P, Tabach Y, Fontemaggi G, Weisz L, Maor-Aloni R, Siegfried Z, Shiff I, Kogan I, Shay M, Kalo E, Blandino G, Simon I, Oren M and Rotter V: Modulation of the vitamin D3 response by cancer-associated mutant p53. Cancer Cell. 17:273–285. 2010. View Article : Google Scholar : PubMed/NCBI

69 

Longley DB, Harkin DP and Johnston PG: 5-fluorouracil: mechanisms of action and clinical strategies. Nat Rev Cancer. 3:330–338. 2003. View Article : Google Scholar : PubMed/NCBI

70 

Shah MA and Schwartz GK: Cell cycle-mediated drug resistance: an emerging concept in cancer therapy. Clin Cancer Res. 7:2168–2181. 2001.PubMed/NCBI

71 

Mader RM, Muler M and Steger G: Resistance to 5-fluorouracil. Gen Pharmacol. 31:661–666. 1998. View Article : Google Scholar

72 

Takagi K, Sowa Y, Cevik OM, Nakanishi R and Sakai T: CDK inhibitor enhances the sensitivity to 5-fluorouracil in colorectal cancer cells. Int J Oncol. 32:1105–1110. 2008.PubMed/NCBI

73 

Watanabe M, Sowa Y, Yogosawa M and Sakai T: Novel MEK inhibitor trametinib and other retinoblastoma gene (RB)-reactivating agents enhance efficacy of 5-fluorouracil on human colon cancer cells. Cancer Sci. 104:687–693. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Milczarek M, Filip-Psurska B, Świętnicki W, Kutner A and Wietrzyk J: Vitamin D analogs combined with 5-fluorouracil in human HT-29 colon cancer treatment. Oncol Rep 32: 491-504, 2014.
APA
Milczarek, M., Filip-Psurska, B., Świętnicki, W., Kutner, A., & Wietrzyk, J. (2014). Vitamin D analogs combined with 5-fluorouracil in human HT-29 colon cancer treatment. Oncology Reports, 32, 491-504. https://doi.org/10.3892/or.2014.3247
MLA
Milczarek, M., Filip-Psurska, B., Świętnicki, W., Kutner, A., Wietrzyk, J."Vitamin D analogs combined with 5-fluorouracil in human HT-29 colon cancer treatment". Oncology Reports 32.2 (2014): 491-504.
Chicago
Milczarek, M., Filip-Psurska, B., Świętnicki, W., Kutner, A., Wietrzyk, J."Vitamin D analogs combined with 5-fluorouracil in human HT-29 colon cancer treatment". Oncology Reports 32, no. 2 (2014): 491-504. https://doi.org/10.3892/or.2014.3247
Copy and paste a formatted citation
x
Spandidos Publications style
Milczarek M, Filip-Psurska B, Świętnicki W, Kutner A and Wietrzyk J: Vitamin D analogs combined with 5-fluorouracil in human HT-29 colon cancer treatment. Oncol Rep 32: 491-504, 2014.
APA
Milczarek, M., Filip-Psurska, B., Świętnicki, W., Kutner, A., & Wietrzyk, J. (2014). Vitamin D analogs combined with 5-fluorouracil in human HT-29 colon cancer treatment. Oncology Reports, 32, 491-504. https://doi.org/10.3892/or.2014.3247
MLA
Milczarek, M., Filip-Psurska, B., Świętnicki, W., Kutner, A., Wietrzyk, J."Vitamin D analogs combined with 5-fluorouracil in human HT-29 colon cancer treatment". Oncology Reports 32.2 (2014): 491-504.
Chicago
Milczarek, M., Filip-Psurska, B., Świętnicki, W., Kutner, A., Wietrzyk, J."Vitamin D analogs combined with 5-fluorouracil in human HT-29 colon cancer treatment". Oncology Reports 32, no. 2 (2014): 491-504. https://doi.org/10.3892/or.2014.3247
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