Establishment of NOD/SCID mouse model of central nervous system leukemia

  • Authors:
    • Dongfang Li
    • Pinggan Li
    • Zhanwen He
    • Zhe Meng
    • Xiangyang Luo
    • Jianpei Fang
  • View Affiliations

  • Published online on: June 12, 2014     https://doi.org/10.3892/or.2014.3254
  • Pages: 684-690
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Abstract

In the present study, we successfully established a NOD/SCID mouse model of central nervous system leukemia by injection of acute monocytic leukemia cell line SHI-1 cells into the lateral ventricle. Immunohistochemistry was used to detect human leukocyte common antigen in brain slices. Nested PCR assay was used to detect MLL/AF6 fusion gene expression. After injection, the condition of the mice gradually progressed to cachexia and death (median survival time, 25 days). Leukemic cells were identified in the lung, bone marrow, and lymph node of one mouse. Brain tissue sections showed invasion into the subdural space, pia mater, arachnoid, along the Virchow-Robin space and into the deep brain parenchyma. In summary, a central nervous system leukemia (CNSL) model was established in NOD/SCID mice.
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August-2014
Volume 32 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Li D, Li P, He Z, Meng Z, Luo X and Fang J: Establishment of NOD/SCID mouse model of central nervous system leukemia. Oncol Rep 32: 684-690, 2014
APA
Li, D., Li, P., He, Z., Meng, Z., Luo, X., & Fang, J. (2014). Establishment of NOD/SCID mouse model of central nervous system leukemia. Oncology Reports, 32, 684-690. https://doi.org/10.3892/or.2014.3254
MLA
Li, D., Li, P., He, Z., Meng, Z., Luo, X., Fang, J."Establishment of NOD/SCID mouse model of central nervous system leukemia". Oncology Reports 32.2 (2014): 684-690.
Chicago
Li, D., Li, P., He, Z., Meng, Z., Luo, X., Fang, J."Establishment of NOD/SCID mouse model of central nervous system leukemia". Oncology Reports 32, no. 2 (2014): 684-690. https://doi.org/10.3892/or.2014.3254