Establishment of NOD/SCID mouse model of central nervous system leukemia

Li,Dongfang; Li,Pinggan; He,Zhanwen; Meng,Zhe; Luo,Xiangyang; Fang,Jianpei

doi:10.3892/or.2014.3254

August-2014 Volume 32 Issue 2

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August-2014 Volume 32 Issue 2

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Article

Establishment of NOD/SCID mouse model of central nervous system leukemia

Authors:
- Dongfang Li
- Pinggan Li
- Zhanwen He
- Zhe Meng
- Xiangyang Luo
- Jianpei Fang
View Affiliations / Copyright

Affiliations: Department of Pediatrics, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510000, P.R. China
Pages: 684-690
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Published online on: June 12, 2014

https://doi.org/10.3892/or.2014.3254
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Abstract

In the present study, we successfully established a NOD/SCID mouse model of central nervous system leukemia by injection of acute monocytic leukemia cell line SHI-1 cells into the lateral ventricle. Immunohistochemistry was used to detect human leukocyte common antigen in brain slices. Nested PCR assay was used to detect MLL/AF6 fusion gene expression. After injection, the condition of the mice gradually progressed to cachexia and death (median survival time, 25 days). Leukemic cells were identified in the lung, bone marrow, and lymph node of one mouse. Brain tissue sections showed invasion into the subdural space, pia mater, arachnoid, along the Virchow-Robin space and into the deep brain parenchyma. In summary, a central nervous system leukemia (CNSL) model was established in NOD/SCID mice.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Establishment of NOD/SCID mouse model of central nervous system leukemia

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