Effect of brachytherapy on NF-κB and VEGF in gastric carcinoma xenografts

  • Authors:
    • Wan-Fu Zhang
    • Wen-Di Jin
    • Bo Li
    • Ming-Chun Wang
    • Xiao-Gang Li
    • Wen-Yuan Mao
    • Kai-Yuan Luo
  • View Affiliations

  • Published online on: June 12, 2014     https://doi.org/10.3892/or.2014.3255
  • Pages: 635-640
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Abstract

Iodine-125 (125I) seed irradiation can be used as an important supplementary treatment for unresectable advanced gastric cancer. However, the radiobiological mechanism underlying brachytherapy remains unclear. Therefore, we investigated the influence of continuous and low-energy 125I irradiation on the cell cycle distribution, apoptosis, expression of NF-κB and VEGF and tumor growth in a human gastric cancer xenograft model. To create an animal model of gastric cancer, SGC-7901 cells were surgically implanted into mice. The 60 mice bearing SGC-7901 gastric cancer xenografts were randomly separated into 2 groups. Sham seeds (0 mCi) were implanted into the control group (n=30); 125I seeds (0.6 mCi) were implanted into the treatment group (n=30). At 28 days after irradiation, apoptosis was detected by flow cytometry. fluorescence micrograph detected intense VEGF and NF-κB immunofluorescence in the tumor samples, and changes in NF-κB and VEGF mRNA and protein expression were assessed by real-time PCR and western blot analysis, respectively. The tumor volume and weight were measured 0-28 days after 125I seed implantation. 125I seed irradiation induced significant apoptosis and G2/M phase arrest. Reduction in the intensities of VEGF and NF-κB immunofluorescence in tumor vessels was observed after treatment. NF-κB and VEGF mRNA and protein expression levels were substantially lower in the implantation treatment group than in the control group. Consequently, 125I seed implantation inhibited cancer growth and reduced cancer volume. The present study revealed that 125I seed irradiation significantly induced apoptosis and cell cycle arrest in the human gastric cancer xenografts. 125I-induced changes in NF-κB and VEGF expression are suggested as potential mechanisms underlying effective brachytherapy.
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August-2014
Volume 32 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang W, Jin W, Li B, Wang M, Li X, Mao W and Luo K: Effect of brachytherapy on NF-κB and VEGF in gastric carcinoma xenografts. Oncol Rep 32: 635-640, 2014
APA
Zhang, W., Jin, W., Li, B., Wang, M., Li, X., Mao, W., & Luo, K. (2014). Effect of brachytherapy on NF-κB and VEGF in gastric carcinoma xenografts. Oncology Reports, 32, 635-640. https://doi.org/10.3892/or.2014.3255
MLA
Zhang, W., Jin, W., Li, B., Wang, M., Li, X., Mao, W., Luo, K."Effect of brachytherapy on NF-κB and VEGF in gastric carcinoma xenografts". Oncology Reports 32.2 (2014): 635-640.
Chicago
Zhang, W., Jin, W., Li, B., Wang, M., Li, X., Mao, W., Luo, K."Effect of brachytherapy on NF-κB and VEGF in gastric carcinoma xenografts". Oncology Reports 32, no. 2 (2014): 635-640. https://doi.org/10.3892/or.2014.3255