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Article

Using [18F]FBAU for imaging brain tumor progression in an F98/tk-luc glioma-bearing rat model

  • Authors:
    • Yi-Chun Chien
    • John Chun-Hao Chen
    • Wei-Chan Lin
    • Hueisch-Jy Ding
    • Hsin-Ell Wang
    • Chih-Hao K. Kao
    • Jeng-Jong Hwang
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Bei-tou 11221, Taipei, Taiwan, R.O.C., Department of Radiology, Cathay General Hospital, Taipei 10630, Taiwan, R.O.C., Department of Medical Imaging and Radiological Sciences, I-Shou University, Jiaosu Village, Kaohsiung 82445, Taiwan, R.O.C., Department of Radiopharmaceutical Production, Buddhist Tzu Chi General Hospital, Hualien 97002, Taiwan, R.O.C.
  • Pages: 691-699
    |
    Published online on: June 12, 2014
       https://doi.org/10.3892/or.2014.3256
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Abstract

1-(2-Deoxy-2-[18F]fluoro-β-D-arabinofuranosyl)-5-bromouracil ([18F]FBAU), a substitute for thymine, has been reported as an effective reporter probe by which to trace cellular metabolism with its positron emission. In the present study, a rat xenograft model bearing F98 glioma transfected with dual reporter genes, herpes simplex virus type 1 thymidine kinase (HSV1-tk) and firefly luciferase (luc) was used for monitoring tumor progression by multimodalities of molecular imaging using [18F]FBAU and D-luciferase as probes. Rat F98 glioma cells were transfected with the pC1-tk-IRES-luc vectors. The selected stable clone was renamed as the F98/tk-luc cell line. Fischer 344 male rats bearing orthotropic F98/tk-luc gliomas in the left brain were used. On day 13 post tumor inoculation, biodistribution, positron emission tomography (PET), magnetic resonance imaging (MRI) and ex vivo autoradiography were performed. The surviving fraction of F98/tk-luc cells treated with 15 µM ganciclovir (GCV) was 15.9%, and the uptake of [131I]FIAU in these cells was significantly enhanced when compared with F98 cells. The correlation coefficient of tumor volume vs. the bioluminescence in the F98/tk-luc glioma-bearing rats was 0.90. The biodistribution showed that the accumulation ratios of [18F]FBAU for glioma-to-normal brain were 9.16, 14.24, 5.7 and 13.7 at 30, 60, 90 and 120 min post i.v. injection, respectively. Consistent tumor enhancement of [18F]FBAU/PET imaging was also noted from 30-90 min post injection. Ex vivo autoradiography also confirmed significant [18F]FBAU uptake in tumors. In conclusion, [18F]FBAU may be used as a PET probe for monitoring glioma progression in animal models and may have potential for clinical use as well.
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1 

Stupp R, Mason WP, van den Bent MJ, et al: Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma. N Engl J Med. 352:987–996. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Sathornsumetee S and Rich JN: Designer therapies for glioblastoma multiforme. Ann NY Acad Sci. 1142:108–132. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Clarke J, Butowski N and Chang S: Recent advances in therapy for glioblastoma. Arch Neurol. 67:279–283. 2010. View Article : Google Scholar

4 

Huang FY, Lee TW, Kao CH, et al: Imaging, autoradiography, and biodistribution of 188Re-labeled PEGylated nanoliposome in orthotopic glioma bearing rat model. Cancer Biother Radiopharm. 26:717–725. 2011.PubMed/NCBI

5 

Miyata S, Kawabata S, Hiramatsu R, et al: Computed tomography imaging of transferrin targeting liposomes encapsulating both boron and iodine contrast agents by convection-enhanced delivery to F98 rat glioma for boron neutron capture therapy. Neurosurgery. 68:1380–1387. 2011.

6 

Wolburg H, Wolburg-Buchholz K, Kraus J, et al: Localization of claudin-3 in tight junctions of the blood-brain barrier is selectively lost during experimental autoimmune encephalomyelitis and human glioblastoma multiforme. Acta Neuropathol. 105:586–592. 2003.

7 

Valk PE, Townsend DW and Maisey MN: Positron Emission Tomography: Basic Science and Clinical Practice. Springer-Verlag Publishing; New York, NY: 2003

8 

Strauss LG: Fluorine-18 deoxyglucose and false-positive results: a major problem in the diagnostics of oncological patients. Eur J Nucl Med. 23:1409–1415. 1996. View Article : Google Scholar : PubMed/NCBI

9 

Kong XB, Zhu QY, Vidal PM, et al: Comparisons of anti-human immunodeficiency virus activities, cellular transport, and plasma and intracellular pharmacokinetics of 3′-fluoro-3′-deoxythymidine and 3′-azido-3′-deoxythymidine. Antimicrob Agents Chemother. 36:808–818. 1992.PubMed/NCBI

10 

Jensen MM, Erichsen KD, Johnbeck CB, et al: [18F]FLT and [18F]FDG PET for non-invasive treatment monitoring of the nicotinamide phosphoribosyltransferase inhibitor APO866 in human xenografts. PLoS One. 8:e534102013.

11 

Tjuvajev JG, Stockhammer G, Desai R, et al: Imaging the expression of transfected genes in vivo. Cancer Res. 55:6126–6132. 1995.PubMed/NCBI

12 

Tjuvajev JG, Finn R, Watanabe K, et al: Noninvasive imaging of herpes virus thymidine kinase gene transfer and expression: a potential method for monitoring clinical gene therapy. Cancer Res. 56:4087–4095. 1996.PubMed/NCBI

13 

Borbath I, Gregoire V, Bergstrom M, Laryea D, Langstrom B and Pauwels S: Use of 5-[76Br]bromo-2′-fluoro-2′-deoxyuridine as a ligand for tumour proliferation: validation in an animal tumour model. Eur J Nucl Med Mol Imaging. 29:19–27. 2002.

14 

Cho SY, Ravasi L, Szajek LP, et al: Evaluation of 76Br-FBAU as a PET reporter probe for HSV1-tk gene expression imaging using mouse models of human glioma. J Nucl Med. 46:1923–1930. 2005.

15 

Kilbourn MR, Dence CS, Welch MJ and Mathias CJ: Fluorine-18 labeling of proteins. J Nucl Med. 28:462–470. 1987.PubMed/NCBI

16 

Gambhir SS, Barrio JR, Herschman HR and Phelps ME: Assays for noninvasive imaging of reporter gene expression. Nucl Med Biol. 26:481–490. 1999. View Article : Google Scholar : PubMed/NCBI

17 

Jacobs AH, Li H, Winkeler A, et al: PET-based molecular imaging in neuroscience. Eur J Nucl Med Mol Imaging. 30:1051–1065. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Pan MH, Huang SC, Liao YP, et al: FLT-PET imaging of radiation responses in murine tumors. Mol Imaging Biol. 10:325–334. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Wang HE, Liao AH, Deng WP, et al: Evaluation of 4-borono-2-18F-fluoro-L-phenylalanine-fructose as a probe for boron neutron capture therapy in a glioma-bearing rat model. J Nucl Med. 45:302–308. 2004.

20 

Wang HE, Wu SY, Chang CW, et al: Evaluation of F-18-labeled amino acid derivatives and [18F]FDG as PET probes in a brain tumor-bearing animal model. Nucl Med Biol. 32:367–375. 2005.

21 

Massoud TF and Gambhir SS: Molecular imaging in living subjects: seeing fundamental biological processes in a new light. Genes Dev. 17:545–580. 2003. View Article : Google Scholar : PubMed/NCBI

22 

De A, Lewis XZ and Gambhir SS: Noninvasive imaging of lentiviral-mediated reporter gene expression in living mice. Mol Ther. 7:681–691. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Bryant MJ, Chuah TL, Luff J, Lavin MF and Walker DG: A novel rat model for glioblastoma multiforme using a bioluminescent F98 cell line. J Clin Neurosci. 15:545–551. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Chang YL, Wang HE, Liu RS, Pang F and Hwang JJ: Monitoring of tumor growth and metastasis potential in MDA-MB-435s/tk-luc human breast cancer xenografts. Nucl Instrum Meth A. 571:155–159. 2007. View Article : Google Scholar

25 

Deng WP, Yang WK, Lai WF, et al: Non-invasive in vivo imaging with radiolabelled FIAU for monitoring cancer gene therapy using herpes simplex virus type 1 thymidine kinase and ganciclovir. Eur J Nucl Med Mol Imaging. 31:99–109. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Alauddin MM, Shahinian A, Park R, Tohme M, Fissekis JD and Conti PS: A general synthesis of 2′-deoxy-2′-[18F]fluoro-5-methyl-1-β-D-arabinofuranosyluracil and its 5-substituted nucleosides. J Labelled Compds Radiopharm. 46:285–289. 2003.

27 

Kao CH, Xie HL, Liao CH, Chen WM and Kao PF: [18F]FBAU 3′,5′-dibenzoate, a lipophilic prodrug, enhances brain uptake of the cell proliferation tracer [18F]FBAU. Nucl Med Biol. 35:635–643. 2008.

28 

Engelhorn T, Eyupoglu IY, Schwarz MA, et al: In vivo micro-CT imaging of rat brain glioma: a comparison with 3T MRI and histology. Neurosci Lett. 458:28–31. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Lin C, Itti E, Haioun C, et al: Early 18F-FDG PET for prediction of prognosis in patients with diffuse large B-cell lymphoma: SUV-based assessment versus visual analysis. J Nucl Med. 48:1626–1632. 2007.

30 

Tjuvajev JG, Avril N, Oku T, et al: Imaging herpes virus thymidine kinase gene transfer and expression by positron emission tomography. Cancer Res. 58:4333–4341. 1998.PubMed/NCBI

31 

Fu DX, Foss CA, Nimmagadda S, Ambinder RF and Pomper MG: Imaging virus-associated cancer. Curr Pharm Des. 14:3048–3065. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Miyagawa T, Gogiberidze G, Serganova I, et al: Imaging of HSV-tk reporter gene expression: comparison between [18F]FEAU, [18F]FFEAU, and other imaging probes. J Nucl Med. 49:637–648. 2008.

33 

Buursma AR, Rutgers V, Hospers GA, Mulder NH, Vaalburg W and de Vries EF: 18F-FEAU as a radiotracer for herpes simplex virus thymidine kinase gene expression: in-vitro comparison with other PET tracers. Nucl Med Commun. 27:25–30. 2006. View Article : Google Scholar

34 

Rehemtulla A, Stegman LD, Cardozo SJ, et al: Rapid and quantitative assessment of cancer treatment response using in vivo bioluminescence imaging. Neoplasia. 2:491–495. 2000. View Article : Google Scholar : PubMed/NCBI

35 

Jost SC, Collins L, Travers S, Piwnica-Worms D and Garbow JR: Measuring brain tumor growth: combined bioluminescence imaging-magnetic resonance imaging strategy. Mol Imaging. 8:245–253. 2009.PubMed/NCBI

36 

Song HT, Jordan EK, Lewis BK, et al: Rat model of metastatic breast cancer monitored by MRI at 3 tesla and bioluminescence imaging with histological correlation. J Transl Med. 7:882009. View Article : Google Scholar : PubMed/NCBI

37 

Spaeth N, Wyss MT, Pahnke J, et al: Uptake of 18F-fluorocholine, 18F-fluoro-ethyl-L-tyrosine and 18F-fluoro-2-deoxyglucose in F98 gliomas in the rat. Eur J Nucl Med Mol Imaging. 33:673–682. 2006.

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Copy and paste a formatted citation
Spandidos Publications style
Chien Y, Chen JC, Lin W, Ding H, Wang H, Kao CK and Hwang J: Using [18F]FBAU for imaging brain tumor progression in an F98/tk-luc glioma-bearing rat model. Oncol Rep 32: 691-699, 2014.
APA
Chien, Y., Chen, J.C., Lin, W., Ding, H., Wang, H., Kao, C.K., & Hwang, J. (2014). Using [18F]FBAU for imaging brain tumor progression in an F98/tk-luc glioma-bearing rat model. Oncology Reports, 32, 691-699. https://doi.org/10.3892/or.2014.3256
MLA
Chien, Y., Chen, J. C., Lin, W., Ding, H., Wang, H., Kao, C. K., Hwang, J."Using [18F]FBAU for imaging brain tumor progression in an F98/tk-luc glioma-bearing rat model". Oncology Reports 32.2 (2014): 691-699.
Chicago
Chien, Y., Chen, J. C., Lin, W., Ding, H., Wang, H., Kao, C. K., Hwang, J."Using [18F]FBAU for imaging brain tumor progression in an F98/tk-luc glioma-bearing rat model". Oncology Reports 32, no. 2 (2014): 691-699. https://doi.org/10.3892/or.2014.3256
Copy and paste a formatted citation
x
Spandidos Publications style
Chien Y, Chen JC, Lin W, Ding H, Wang H, Kao CK and Hwang J: Using [18F]FBAU for imaging brain tumor progression in an F98/tk-luc glioma-bearing rat model. Oncol Rep 32: 691-699, 2014.
APA
Chien, Y., Chen, J.C., Lin, W., Ding, H., Wang, H., Kao, C.K., & Hwang, J. (2014). Using [18F]FBAU for imaging brain tumor progression in an F98/tk-luc glioma-bearing rat model. Oncology Reports, 32, 691-699. https://doi.org/10.3892/or.2014.3256
MLA
Chien, Y., Chen, J. C., Lin, W., Ding, H., Wang, H., Kao, C. K., Hwang, J."Using [18F]FBAU for imaging brain tumor progression in an F98/tk-luc glioma-bearing rat model". Oncology Reports 32.2 (2014): 691-699.
Chicago
Chien, Y., Chen, J. C., Lin, W., Ding, H., Wang, H., Kao, C. K., Hwang, J."Using [18F]FBAU for imaging brain tumor progression in an F98/tk-luc glioma-bearing rat model". Oncology Reports 32, no. 2 (2014): 691-699. https://doi.org/10.3892/or.2014.3256
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