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Article

Vitamin C enhances anticancer activity in methotrexate‑treated Hep3B hepatocellular carcinoma cells

  • Authors:
    • Giou-Teng Yiang
    • Pei-Lun Chou
    • Yu-Ting Hung
    • Jen-Ni Chen
    • Wei-Jung Chang
    • Yung-Luen Yu
    • Chyou-Wei Wei
  • View Affiliations / Copyright

    Affiliations: Department of Emergency Medicine, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei 231, Taiwan, R.O.C., Division of Allergy-Immunology-Rheumatology, Department of Internal Medicine, Saint Mary's Hospital Luodong, Yilan 265, Taiwan, R.O.C., Department of Nutrition, Master Program of Biomedical Nutrition, Hungkuang University, Shalu, Taichung 433, Taiwan, R.O.C., Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 402, Taiwan, R.O.C., Graduate Institute of Cancer Biology, and Center for Molecular Medicine, China Medical University, Taichung 404, Taiwan, R.O.C.
  • Pages: 1057-1063
    |
    Published online on: June 25, 2014
       https://doi.org/10.3892/or.2014.3289
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Abstract

Methotrexate (MTX) has been widely used for rheumatoid arthritis therapy for a long time. MTX is also used as an anticancer drug for various tumors. However, many studies have shown that high-dose MTX treatment for cancer therapy may cause liver and renal damage. Alhough the mechanisms involved in MTX-induced liver and renal damage require further research, many studies have indicated that MTX-induced cytotoxicity is associated with increases in oxidative stress and caspase activation. In order to reduce MTX-induced side-effects and increase anticancer efficiency, currently, combination treatments of low-dose MTX and other anticancer drugs are considered and applied for various tumor treatments. The present study showed that MTX induces increases in H2O2 levels and caspase-9/-3 activation leading to cell death in hepatocellular carcinoma Hep3B cells. Importantly, this study is the first to demonstrate that vitamin C can efficiently aid low-dose MTX in inducing cell death in Hep3B cells. Therefore, the present study provides a possible powerful therapeutic method for tumors using a combined treatment of vitamin C and low-dose MTX.
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1 

Weinblatt ME: Methotrexate in rheumatoid arthritis: a quarter century of development. Trans Am Clin Climatol Assoc. 124:16–25. 2013.PubMed/NCBI

2 

Patane M, Ciriaco M, Chimirri S, et al: Interactions among low dose of methotrexate and drugs used in the treatment of rheumatoid arthritis. Adv Pharmacol Sci. 2013:3138582013.PubMed/NCBI

3 

Cakir T, Ozkan E, Dulundu E, et al: Caffeic acid phenethyl ester (CAPE) prevents methotrexate-induced hepatorenal oxidative injury in rats. J Pharm Pharmacol. 63:1566–1571. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Keystone E, Landewe R, van Vollenhoven R, et al: Long-term safety and efficacy of certolizumab pegol in combination with methotrexate in the treatment of rheumatoid arthritis: 5-year results from the RAPID 1 trial and open-label extension. Ann Rheum Dis. Aug 5–2013.(Epub ahead of print). View Article : Google Scholar

5 

Cordero MD, Sanchez-Alcazar JA, Bautista-Ferrufino MR, et al: Acute oxidant damage promoted on cancer cells by amitriptyline in comparison with some common chemotherapeutic drugs. Anticancer Drugs. 21:932–944. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Otrocka M, Verschueren H and Malicka-Blaszkiewicz M: The effect of methotrexate on actin and invasiveness of hepatoma Morris 5123 cells in culture. Acta Biochim Pol. 48:1051–1060. 2001.PubMed/NCBI

7 

Jolivet J, Cowan KH, Curt GA, Clendeninn NJ and Chabner BA: The pharmacology and clinical use of methotrexate. N Engl J Med. 309:1094–1104. 1983. View Article : Google Scholar : PubMed/NCBI

8 

Takemura Y and Jackman AL: Folate-based thymidylate synthase inhibitors in cancer chemotherapy. Anticancer Drugs. 8:3–16. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Shirao K, Boku N, Yamada Y, et al: Randomized phase III study of 5-fluorouracil continuous infusion vs. sequential methotrexate and 5-fluorouracil therapy in far advanced gastric cancer with peritoneal metastasis (JCOG0106). Jpn J Clin Oncol. 43:972–980. 2013. View Article : Google Scholar

10 

Chen Y, Zhang W, Gu J, et al: Enhanced antitumor efficacy by methotrexate conjugated Pluronic mixed micelles against KBv multidrug resistant cancer. Int J Pharm. 452:421–433. 2013. View Article : Google Scholar

11 

Ding L, Hu XM, Wu H, et al: Combined transfection of Bcl-2 siRNA and miR-15a oligonucleotides enhanced methotrexate-induced apoptosis in Raji cells. Cancer Biol Med. 10:16–21. 2013.PubMed/NCBI

12 

Florou D, Patsis C, Ardavanis A and Scorilas A: Effect of doxorubicin, oxaliplatin, and methotrexate administration on the transcriptional activity of BCL-2 family gene members in stomach cancer cells. Cancer Biol Ther. 14:587–596. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Saez-Ayala M, Montenegro MF, Sanchez-Del-Campo L, et al: Directed phenotype switching as an effective antimelanoma strategy. Cancer Cell. 24:105–119. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Yan KH, Lee LM, Hsieh MC, et al: Aspirin antagonizes the cytotoxic effect of methotrexate in lung cancer cells. Oncol Rep. 30:1497–1505. 2013.PubMed/NCBI

15 

Ohgidani M, Komizu Y, Goto K and Ueoka R: Residual powders from Shochu distillation remnants induce apoptosis in human hepatoma cells via the caspase-independent pathway. J Biosci Bioeng. 114:104–109. 2012.PubMed/NCBI

16 

Yu VW and Ho WS: Tetrandrine inhibits hepatocellular carcinoma cell growth through the caspase pathway and G2/M phase. Oncol Rep. 29:2205–2210. 2013.PubMed/NCBI

17 

Fan CM, Foster BK, Hui SK and Xian CJ: Prevention of bone growth defects, increased bone resorption and marrow adiposity with folinic acid in rats receiving long-term methotrexate. PloS One. 7:e469152012. View Article : Google Scholar : PubMed/NCBI

18 

Okamura M, Hashimoto K, Shimada J and Sakagami H: Apoptosis-inducing activity of cisplatin (CDDP) against human hepatoma and oral squamous cell carcinoma cell lines. Anticancer Res. 24:655–661. 2004.PubMed/NCBI

19 

Papachristopoulou G, Talieri M and Scorilas A: Significant alterations in the expression pattern of kallikrein-related peptidase genes KLK4, KLK5 and KLK14 after treatment of breast cancer cells with the chemotherapeutic agents epirubicin, docetaxel and methotrexate. Tumour Biol. 34:369–378. 2013. View Article : Google Scholar

20 

Wehner R, Bitterlich A, Meyer N, et al: Impact of chemotherapeutic agents on the immunostimulatory properties of human 6-sulfo LacNAc+(slan) dendritic cells. Int J Cancer. 132:1351–1359. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Zhang D and Loughran TP Jr: Large granular lymphocytic leukemia: molecular pathogenesis, clinical manifestations, and treatment. Hematology Am Soc Hematol Educ Program. 2012.652–659. 2012.PubMed/NCBI

22 

Xie XB, Yin JQ, Wen LL, et al: Critical role of heat shock protein 27 in bufalin-induced apoptosis in human osteosarcomas: a proteomic-based research. PloS One. 7:e473752012. View Article : Google Scholar : PubMed/NCBI

23 

Yang TM, Qi SN, Zhao N, et al: Induction of apoptosis through caspase-independent or caspase-9-dependent pathway in mouse and human osteosarcoma cells by a new nitroxyl spin-labeled derivative of podophyllotoxin. Apoptosis. 18:727–738. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Green DR and Reed JC: Mitochondria and apoptosis. Science. 281:1309–1312. 1998. View Article : Google Scholar : PubMed/NCBI

25 

Thornberry NA and Lazebnik Y: Caspases: enemies within. Science. 281:1312–1316. 1998. View Article : Google Scholar : PubMed/NCBI

26 

Tang CH, Hu CC, Wei CW and Wang JJ: Synergism of Rana catesbeiana ribonuclease and IFN-γ triggers distinct death machineries in different human cancer cells. FEBS Lett. 579:265–270. 2005.

27 

Yaoxian W, Hui Y, Yunyan Z, Yanqin L, Xin G and Xiaoke W: Emodin induces apoptosis of human cervical cancer hela cells via intrinsic mitochondrial and extrinsic death receptor pathway. Cancer Cell Int. 13:712013. View Article : Google Scholar : PubMed/NCBI

28 

Beaudouin J, Liesche C, Aschenbrenner S, Horner M and Eils R: Caspase-8 cleaves its substrates from the plasma membrane upon CD95-induced apoptosis. Cell Death Differ. 20:599–610. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Yu YL, Wei CW, Chen YL, Chen MH and Yiang GT: Immunotherapy of breast cancer by single delivery with rAAV2-mediated interleukin-15 expression. Int J Oncol. 36:365–370. 2010.PubMed/NCBI

30 

Zheng B, Wu L, Ma L, et al: Telekin induces apoptosis associated with the mitochondria-mediated pathway in human hepatocellular carcinoma cells. Biol Pharm Bull. 36:1118–1125. 2013. View Article : Google Scholar

31 

Yiang GT, Harn HJ, Yu YL, et al: Immunotherapy: rAAV2 expressing interleukin-15 inhibits HeLa cell tumor growth in mice. J Biomed Sci. 16:472009. View Article : Google Scholar : PubMed/NCBI

32 

Chen YX, Lv WG, Chen HZ, Ye F and Xie X: Methotrexate induces apoptosis of human choriocarcinoma cell line JAR via a mitochondrial pathway. Eur J Obstet Gynecol Reprod Biol. 143:107–111. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Shiu LY, Chang LC, Liang CH, Huang YS, Sheu HM and Kuo KW: Solamargine induces apoptosis and sensitizes breast cancer cells to cisplatin. Food Chem Toxicol. 45:2155–2164. 2007. View Article : Google Scholar : PubMed/NCBI

34 

Ehrhardt H, Wachter F, Maurer M, Stahnke K and Jeremias I: Important role of caspase-8 for chemosensitivity of ALL cells. Clin Cancer Res. 17:7605–7613. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Caglar Y, Ozgur H, Matur I, et al: Ultrastructural evaluation of the effect of N-acetylcysteine on methotrexate nephrotoxicity in rats. Histol Histopathol. 28:865–874. 2013.PubMed/NCBI

36 

Kolli VK, Abraham P, Isaac B and Selvakumar D: Neutrophil infiltration and oxidative stress may play a critical role in methotrexate-induced renal damage. Chemotherapy. 55:83–90. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Tunali-Akbay T, Sehirli O, Ercan F and Sener G: Resveratrol protects against methotrexate-induced hepatic injury in rats. J Pharm Pharm Sci. 13:303–310. 2010.PubMed/NCBI

38 

Ciralik H, Bulbuloglu E, Cetinkaya A, Kurutas EB, Celik M and Polat A: Effects of N-acetylcysteine on methotrexate-induced small intestinal damage in rats. Mt Sinai J Med. 73:1086–1092. 2006.PubMed/NCBI

39 

Cetinkaya A, Kurutas EB, Bulbuloglu E and Kantarceken B: The effects of N-acetylcysteine on methotrexate-induced oxidative renal damage in rats. Nephrol Dial Transplant. 22:284–285. 2007. View Article : Google Scholar : PubMed/NCBI

40 

Wu CS, Yen CJ, Chou RH, et al: Cancer-associated carbohydrate antigens as potential biomarkers for hepatocellular carcinoma. PloS One. 7:e394662012. View Article : Google Scholar : PubMed/NCBI

41 

Pyrhonen S, Kuitunen T, Nyandoto P and Kouri M: Randomised comparison of fluorouracil, epidoxorubicin and methotrexate (FEMTX) plus supportive care with supportive care alone in patients with non-resectable gastric cancer. Br J Cancer. 71:587–591. 1995. View Article : Google Scholar : PubMed/NCBI

42 

Lu Y, Sun J, Petrova K, et al: Metabolomics evaluation of the effects of green tea extract on acetaminophen-induced hepatotoxicity in mice. Food Chem Toxicol. 62:707–721. 2013. View Article : Google Scholar : PubMed/NCBI

43 

Mukhopadhyay PK, Dey A, Mukherjee S and Pradhan NK: The effect of coadministration of α-tocopherol and ascorbic acid on arsenic trioxide-induced testicular toxicity in adult rats. J Basic Clin Physiol Pharmacol. 24:245–253. 2013.

44 

Kim H, Bae S, Kim Y, et al: Vitamin C prevents stress-induced damage on the heart caused by the death of cardiomyocytes, through down-regulation of the excessive production of catecholamine, TNF-α, and ROS production in Gulo(−/−) mice. Free Radic Biol Med. 65C:573–583. 2013.PubMed/NCBI

45 

Eroglu S, Pandir D, Uzun FG and Bas H: Protective role of vitamins C and E in diclorvos-induced oxidative stress in human erythrocytes in vitro. Biol Res. 46:33–38. 2013. View Article : Google Scholar : PubMed/NCBI

46 

Murata W, Tanaka T, Kubo I and Fujita K: Protective effects of alpha-tocopherol and ascorbic acid against cardol-induced cell death and reactive oxygen species generation in Staphylococcus aureus. Planta Med. 79:768–774. 2013. View Article : Google Scholar : PubMed/NCBI

47 

Hattiwale SH, Saha S, Yendigeri SM, Jargar JG, Dhundasi SA and Das KK: Protective effect of L-ascorbic acid on nickel induced pulmonary nitrosative stress in male albino rats. Biometals. 26:329–336. 2013. View Article : Google Scholar : PubMed/NCBI

48 

Alexander B, Fishman AI, Eshghi M, Choudhury M and Konno S: Induction of cell death in renal cell carcinoma with combination of D-fraction and vitamin C. Integr Cancer Ther. 12:442–448. 2013. View Article : Google Scholar : PubMed/NCBI

49 

Nagappan A, Park KI, Park HS, et al: Vitamin C induces apoptosis in AGS cells by down-regulation of 14-3-3σ via a mitochondrial dependent pathway. Food Chem. 135:1920–1928. 2012.PubMed/NCBI

50 

Vetvicka V and Vetvickova J: Combination of glucan, resveratrol and vitamin C demonstrates strong anti-tumor potential. Anticancer Res. 32:81–87. 2012.PubMed/NCBI

51 

Lee WJ: The prospects of vitamin C in cancer therapy. Immune Netw. 9:147–152. 2009. View Article : Google Scholar : PubMed/NCBI

52 

Li W, Wu JX and Tu YY: Synergistic effects of tea polyphenols and ascorbic acid on human lung adenocarcinoma SPC-A-1 cells. J Zhejiang Univ Sci B. 11:458–464. 2010. View Article : Google Scholar : PubMed/NCBI

53 

Wei CW, Lin CC, Yu YL, et al: n-Butylidenephthalide induced apoptosis in the A549 human lung adenocarcinoma cell line by coupled down-regulation of AP-2α and telomerase activity. Acta Pharmacol Sin. 30:1297–1306. 2009. View Article : Google Scholar

54 

Yu YL, Su KJ, Chen CJ, et al: Synergistic anti-tumor activity of isochaihulactone and paclitaxel on human lung cancer cells. J Cell Physiol. 227:213–222. 2012. View Article : Google Scholar : PubMed/NCBI

55 

Yiang GT, Chen YH, Chou PL, Chang WJ, Wei CW and Yu YL: The NS3 protease and helicase domains of Japanese encephalitis virus trigger cell death via caspase dependent and independent pathways. Mol Med Rep. 7:826–830. 2013.PubMed/NCBI

56 

Yiang GT, Yu YL, Hu SC, Chen MH, Wang JJ and Wei CW: PKC and MEK pathways inhibit caspase-9/-3-mediated cytotoxicity in differentiated cells. FEBS Lett. 582:881–885. 2008. View Article : Google Scholar : PubMed/NCBI

57 

Chen KH, Li PC, Lin WH, Chien CT and Low BH: Depression by a green tea extract of alcohol-induced oxidative stress and lipogenesis in rat liver. Biosci Biotechnol Biochem. 75:1668–1676. 2011. View Article : Google Scholar : PubMed/NCBI

58 

Lin BR, Yu CJ, Chen WC, et al: Green tea extract supplement reduces D-galactosamine-induced acute liver injury by inhibition of apoptotic and proinflammatory signaling. J Biomed Sci. 16:352009. View Article : Google Scholar : PubMed/NCBI

59 

Islam MS, Haq SA, Islam MN, et al: Comparative efficacy of subcutaneous versus oral methotrexate in active rheumatoid arthritis. Mymensingh Med J. 22:483–488. 2013.PubMed/NCBI

60 

Yiang GT, Yu YL, Chou PL, et al: The cytotoxic protein can induce autophagocytosis in addition to apoptosis in MCF-7 human breast cancer cells. In Vivo; 26:403–409. 2012.PubMed/NCBI

61 

Wei CW, Hu CC, Tang CH, Lee MC and Wang JJ: Induction of differentiation rescues HL-60 cells from Rana catesbeiana ribonuclease-induced cell death. FEBS Lett. 531:421–426. 2002. View Article : Google Scholar : PubMed/NCBI

62 

Cetinkaya A, Bulbuloglu E, Kurutas EB and Kantarceken B: N-acetylcysteine ameliorates methotrexate-induced oxidative liver damage in rats. Med Sci Monit. 12:BR274–BR278. 2006.PubMed/NCBI

63 

Chen J, Xiao XQ, Deng CM, Su XS and Li GY: Downregulation of xIAP expression by small interfering RNA inhibits cellular viability and increases chemosensitivity to methotrexate in human hepatoma cell line HepG2. J Chemother. 18:525–531. 2006. View Article : Google Scholar : PubMed/NCBI

64 

Miller KD, Loehrer PJ, Gonin R, et al: A phase II study of weekly oral methotrexate and zidovudine (AZT) in advanced adenocarcinoma of the pancreas and hepatocellular carcinoma. Invest New Drugs. 14:207–212. 1996. View Article : Google Scholar : PubMed/NCBI

65 

Muller M, Strand S, Hug H, et al: Drug-induced apoptosis in hepatoma cells is mediated by the CD95 (APO-1/Fas) receptor/ligand system and involves activation of wild-type p53. J Clin Invest. 99:403–413. 1997. View Article : Google Scholar : PubMed/NCBI

66 

Wang Y, Chen H, Liu Y, et al: pH-sensitive pullulan-based nanoparticle carrier of methotrexate and combretastatin A4 for the combination therapy against hepatocellular carcinoma. Biomaterials. 34:7181–7190. 2013. View Article : Google Scholar : PubMed/NCBI

67 

Zhao R, Hanscom M and Goldman ID: The relationship between folate transport activity at low pH and reduced folate carrier function in human Huh7 hepatoma cells. Biochim Biophys Acta. 1715:57–64. 2005. View Article : Google Scholar : PubMed/NCBI

68 

Aden DP, Fogel A, Plotkin S, Damjanov I and Knowles BB: Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line. Nature. 282:615–616. 1979. View Article : Google Scholar : PubMed/NCBI

69 

Ponchel F, Puisieux A, Tabone E, et al: Hepatocarcinoma-specific mutant 53-249ser induces mitotic activity but has no effect on transforming growth factor β 1-mediated apoptosis. Cancer Res. 54:2064–2068. 1994.PubMed/NCBI

70 

Held LA, Rizzieri D, Long GD, et al: A Phase I study of arsenic trioxide (Trisenox), ascorbic acid, and bortezomib (Velcade) combination therapy in patients with relapsed/refractory multiple myeloma. Cancer Invest. 31:172–176. 2013. View Article : Google Scholar

71 

Volta V, Ranzato E, Martinotti S, et al: Preclinical demonstration of synergistic active nutrients/drug (AND) combination as a potential treatment for malignant pleural mesothelioma. PloS One. 8:e580512013. View Article : Google Scholar : PubMed/NCBI

72 

Vuyyuri SB, Rinkinen J, Worden E, Shim H, Lee S and Davis KR: Ascorbic acid and a cytostatic inhibitor of glycolysis synergistically induce apoptosis in non-small cell lung cancer cells. PloS One. 8:e670812013. View Article : Google Scholar : PubMed/NCBI

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Yiang G, Chou P, Hung Y, Chen J, Chang W, Yu Y and Wei C: Vitamin C enhances anticancer activity in methotrexate‑treated Hep3B hepatocellular carcinoma cells. Oncol Rep 32: 1057-1063, 2014.
APA
Yiang, G., Chou, P., Hung, Y., Chen, J., Chang, W., Yu, Y., & Wei, C. (2014). Vitamin C enhances anticancer activity in methotrexate‑treated Hep3B hepatocellular carcinoma cells. Oncology Reports, 32, 1057-1063. https://doi.org/10.3892/or.2014.3289
MLA
Yiang, G., Chou, P., Hung, Y., Chen, J., Chang, W., Yu, Y., Wei, C."Vitamin C enhances anticancer activity in methotrexate‑treated Hep3B hepatocellular carcinoma cells". Oncology Reports 32.3 (2014): 1057-1063.
Chicago
Yiang, G., Chou, P., Hung, Y., Chen, J., Chang, W., Yu, Y., Wei, C."Vitamin C enhances anticancer activity in methotrexate‑treated Hep3B hepatocellular carcinoma cells". Oncology Reports 32, no. 3 (2014): 1057-1063. https://doi.org/10.3892/or.2014.3289
Copy and paste a formatted citation
x
Spandidos Publications style
Yiang G, Chou P, Hung Y, Chen J, Chang W, Yu Y and Wei C: Vitamin C enhances anticancer activity in methotrexate‑treated Hep3B hepatocellular carcinoma cells. Oncol Rep 32: 1057-1063, 2014.
APA
Yiang, G., Chou, P., Hung, Y., Chen, J., Chang, W., Yu, Y., & Wei, C. (2014). Vitamin C enhances anticancer activity in methotrexate‑treated Hep3B hepatocellular carcinoma cells. Oncology Reports, 32, 1057-1063. https://doi.org/10.3892/or.2014.3289
MLA
Yiang, G., Chou, P., Hung, Y., Chen, J., Chang, W., Yu, Y., Wei, C."Vitamin C enhances anticancer activity in methotrexate‑treated Hep3B hepatocellular carcinoma cells". Oncology Reports 32.3 (2014): 1057-1063.
Chicago
Yiang, G., Chou, P., Hung, Y., Chen, J., Chang, W., Yu, Y., Wei, C."Vitamin C enhances anticancer activity in methotrexate‑treated Hep3B hepatocellular carcinoma cells". Oncology Reports 32, no. 3 (2014): 1057-1063. https://doi.org/10.3892/or.2014.3289
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