MiR-449a functions as a tumor suppressor in endometrial cancer by targeting CDC25A

  • Authors:
    • Wenwei Ye
    • Jisen Xue
    • Qian Zhang
    • Fuyao Li
    • Wei Zhang
    • Huijun Chen
    • Yibo Huang
    • Feiyun Zheng
  • View Affiliations

  • Published online on: July 3, 2014     https://doi.org/10.3892/or.2014.3303
  • Pages: 1193-1199
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Abstract

Accumulating evidence has demonstrated that microRNAs (miRNAs) play critical roles in cancer initiation and development by functioning either as oncogenes or as tumor suppressors. The role of microRNA-449a (miR-449a) in endometrial cancer remains unclear. We examined the levels of miR-449a and miR-449b in benign endometrium, type I and type II endometrial cancer tissues by quantitative real-time polymerase chain reaction. To further investigate the roles of miR-449a in regulating the behavior of endometrial cancer cells, we overexpressed miR-449a in the endometrial cancer cell line HEC-1B, which had low endogenous miR-449a expression. We analyzed the effects of miR-449a overexpression on CDC25 expression, proliferation, invasion and apoptosis of HEC-1B cells. We found that miR-449a and miR-449b levels were markedly reduced in type II endometrial cancer tissues but not in type I endometrial cancer tissues compared with normal endometrium. Overexpression of miR-449a significantly inhibited the proliferation, invasion and clonogenic survival of HEC-1B cells. MiR-449a overexpression also induced apoptosis in HEC-1B cells. In addition, real-time RT-PCR and western blot analysis showed that CDC25A expression was suppressed by miR-449a overexpression. Our results suggest that miR-449a may act as a tumor suppressor by targeting CDC25A in endometrial cancer.
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September-2014
Volume 32 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ye W, Xue J, Zhang Q, Li F, Zhang W, Chen H, Huang Y and Zheng F: MiR-449a functions as a tumor suppressor in endometrial cancer by targeting CDC25A. Oncol Rep 32: 1193-1199, 2014
APA
Ye, W., Xue, J., Zhang, Q., Li, F., Zhang, W., Chen, H. ... Zheng, F. (2014). MiR-449a functions as a tumor suppressor in endometrial cancer by targeting CDC25A. Oncology Reports, 32, 1193-1199. https://doi.org/10.3892/or.2014.3303
MLA
Ye, W., Xue, J., Zhang, Q., Li, F., Zhang, W., Chen, H., Huang, Y., Zheng, F."MiR-449a functions as a tumor suppressor in endometrial cancer by targeting CDC25A". Oncology Reports 32.3 (2014): 1193-1199.
Chicago
Ye, W., Xue, J., Zhang, Q., Li, F., Zhang, W., Chen, H., Huang, Y., Zheng, F."MiR-449a functions as a tumor suppressor in endometrial cancer by targeting CDC25A". Oncology Reports 32, no. 3 (2014): 1193-1199. https://doi.org/10.3892/or.2014.3303