Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
September-2014 Volume 32 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2014 Volume 32 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

IGF expression in HPV-related and HPV-unrelated human cancer cells

  • Authors:
    • Julia Durzyńska
    • Elisabeth Barton
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Virology, Faculty of Biology, Institute of Experimental Biology, Adam Mickiewicz University, 61‑614 Poznań, Poland, Department of Anatomy and Cell Biology, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Copyright: © Durzyńska et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 893-900
    |
    Published online on: July 11, 2014
       https://doi.org/10.3892/or.2014.3329
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The human Igf-1 gene not only produces insulin‑like growth factor-I (IGF-I), but also different carboxy‑terminal extensions, known as E peptides, through alternative splicing. We and others have shown that human Eb peptide (hEb) derived from Igf-1 has intrinsic biological activity and is localized to nuclei of transfected cells. Since hEb actions can complement the activity of IGF-I itself, the aim of the present study was to compare IGF-I isoforms at the endogenous protein and transcript level in cancer cell lines, including HeLa, U2OS, HepG2 and K562 cells. Quantitative real-time PCR (qRT‑PCR) using Igf-1 isoform specific primers was performed to determine expression patterns, using β-actin as a reference gene. The overall relative Igf-1 transcript level was different across the cell lines, with ~80-fold higher expression in K562 (130.2±31.2) than in U2OS cells (1.7±1.1). The relative copy number of Igf-1b was the highest in HepG2 (69.9±28.6) and K562 cells (28.3±6.7), whereas the relative copy numbers of Igf-1a and Igf-1c were significantly higher in K562 cells compared to all other cell lines. Immunoblotting using total cell lysates, cytoplasmic and nuclear fractions were carried out to determine the level and distribution of IGF-I proteins. K562 cells exhibited the highest level of hEb in total cell lysates and nuclear fractions and no cell lines displayed hEb in the cytoplasmic fractions. In contrast, IGF-IA was the highest in HeLa cells and was enriched only in the cytoplasmic fraction. Since relatively low IGF-1A transcript level but relatively high pro‑IGF-1A protein level is plausible, we hypothesized that these transcripts could be processed with higher efficiency and/or the protein product may be stabilized by viral HPV oncogenes in HeLa cells. We assert that while it is important to analyze Igf-1 transcript level, it may be more relevant to determine the IGF isoforms at the protein level.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Brett D, Pospisil H, Valcárcel J, Reich J and Bork P: Alternative splicing and genome complexity. Nat Genet. 30:29–30. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Roy B, Haupt LM and Griffiths LR: Review: alternative splicing (AS) of genes as an approach for generating protein complexity. Curr Genomics. 14:182–194. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Renaville R, Hammadi M and Portetelle D: Role of the somatotropic axis in the mammalian metabolism. Domest Anim Endocrinol. 23:351–360. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Langdahl BL, Kassem M, Møller MK and Eriksen EF: The effects of IGF-I and IGF-II on proliferation and differentiation of human osteoblasts and interactions with growth hormone. Eur J Clin Invest. 28:176–183. 1998. View Article : Google Scholar : PubMed/NCBI

5 

Siegfried JM, Kasprzyk PG, Treston AM, Mulshine JL, Quinn KA and Cuttitta F: A mitogenic peptide amide encoded within the E peptide domain of the insulin-like growth factor IB prohormone. Proc Natl Acad Sci USA. 89:8107–8111. 1992. View Article : Google Scholar : PubMed/NCBI

6 

Kuo YH and Chen TT: Novel activities of pro-IGF-I E peptides: regulation of morphological differentiation and anchorage-independent growth in human neuroblastoma cells. Exp Cell Res. 280:75–89. 2002. View Article : Google Scholar

7 

Matheny RW Jr and Nindl BC: Loss of IGF-IEa or IGF-IEb impairs myogenic differentiation. Endocrinology. 152:1923–1934. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Hede MS, Salimova E, Piszczek A, et al: E-peptides control bioavailability of IGF-1. PLoS One. 7:e511522012. View Article : Google Scholar : PubMed/NCBI

9 

Barton ER: The ABCs of IGF-I isoforms: impact on muscle hypertrophy and implications for repair. Appl Physiol Nutr Metab. 31:791–797. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Tian XC, Chen MJ, Pantschenko AG, Yang TJ and Chen TT: Recombinant E-peptides of pro-IGF-I have mitogenic activity. Endocrinology. 140:3387–3390. 1999. View Article : Google Scholar : PubMed/NCBI

11 

Brisson BK and Barton ER: New modulators for IGF-I activity within IGF-I processing products. Front Endocrinol (Lausanne). 4:422013.PubMed/NCBI

12 

Durzyńska J, Philippou A, Brisson BK, Nguyen-McCarty M and Barton ER: The pro-forms of insulin-like growth factor I (IGF-I) are predominant in skeletal muscle and alter IGF-I receptor activation. Endocrinology. 154:1215–1224. 2013.PubMed/NCBI

13 

Tan DS, Cook A and Chew SL: Nucleolar localization of an isoform of the IGF-I precursor. BMC Cell Biol. 3:172002. View Article : Google Scholar : PubMed/NCBI

14 

Durzyńska J, Wardziński A, Koczorowska M, Goździcka-Józefiak A and Barton ER: Human Eb peptide: not just a by-product of pre-pro-IGF1b processing? Horm Metab Res. 45:415–422. 2013.PubMed/NCBI

15 

Brisson BK and Barton ER: Insulin-like growth factor-I E-peptide activity is dependent on the IGF-I receptor. PLoS One. 7:e455882012. View Article : Google Scholar : PubMed/NCBI

16 

Aro AL, Savikko J, Pulkkinen V and von Willebrand E: Expression of insulin-like growth factors IGF-I and IGF-II, and their receptors during the growth and megakaryocytic differentiation of K562 cells. Leuk Res. 26:831–837. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Wahner Hendrickson AE, Haluska P, Schneider PA, et al: Expression of insulin receptor isoform A and insulin-like growth factor-1 receptor in human acute myelogenous leukemia: effect of the dual-receptor inhibitor BMS-536924 in vitro. Cancer Res. 69:7635–7643. 2009.PubMed/NCBI

18 

Philippou A, Stavropoulou A, Sourla A, et al: Characterization of a rabbit antihuman mechano growth factor (MGF) polyclonal antibody against the last 24 amino acids of the E domain. In Vivo; 22:27–35. 2008.PubMed/NCBI

19 

Harris JR and Markl J: Keyhole limpet hemocyanin (KLH): a biomedical review. Micron. 30:597–623. 1999. View Article : Google Scholar : PubMed/NCBI

20 

Lowe WL Jr, Lasky SR, LeRoith D and Roberts CT Jr: Distribution and regulation of rat insulin-like growth factor I messenger ribonucleic acids encoding alternative carboxyterminal E-peptides: evidence for differential processing and regulation in liver. Mol Endocrinol. 2:528–535. 1988. View Article : Google Scholar

21 

Wallis M: New insulin-like growth factor (IGF)-precursor sequences from mammalian genomes: the molecular evolution of IGFs and associated peptides in primates. Growth Horm IGF Res. 19:12–23. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Pfeffer LA, Brisson BK, Lei H and Barton ER: The insulin-like growth factor (IGF)-I E-peptides modulate cell entry of the mature IGF-I protein. Mol Biol Cell. 20:3810–3817. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Adamo ML, Neuenschwander S, LeRoith D and Roberts CT Jr: Structure, expression, and regulation of the IGF-I gene. Adv Exp Med Biol. 343:1–11. 1993. View Article : Google Scholar : PubMed/NCBI

24 

Kasprzak A, Szaflarski W, Szmeja J, et al: Differential expression of IGF-1 mRNA isoforms in colorectal carcinoma and normal colon tissue. Int J Oncol. 42:305–316. 2013.PubMed/NCBI

25 

Kasprzak A, Szaflarski W, Szmeja J, et al: Expression of various insulin-like growth factor-1 mRNA isoforms in colorectal cancer. Contemp Oncol (Pozn). 16:147–153. 2012.PubMed/NCBI

26 

Koczorowska MM, Kwasniewska A and Gozdzicka-Jozefiak A: IGF1 mRNA isoform expression in the cervix of HPV-positive women with pre-cancerous and cancer lesions. Exp Ther Med. 2:149–156. 2011.PubMed/NCBI

27 

Cui H, Yi Q, Feng J, Yang L and Tang L: Mechano growth factor E peptide regulates migration and differentiation of bone marrow mesenchymal stem cells. J Mol Endocrinol. 52:111–120. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Wu J, Wu K, Lin F, et al: Mechano-growth factor induces migration of rat mesenchymal stem cells by altering its mechanical properties and activating ERK pathway. Biochem Biophys Res Commun. 441:202–207. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Fornaro M, Hinken AC, Needle S, et al: Mechano-growth factor peptide, the COOH terminus of unprocessed insulin-like growth factor 1, has no apparent effect on myoblasts or primary muscle stem cells. Am J Physiol Endocrinol Metab. 306:E150–E156. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Armakolas A, Philippou A, Panteleakou Z, et al: Preferential expression of IGF-1Ec (MGF) transcript in cancerous tissues of human prostate: evidence for a novel and autonomous growth factor activity of MGF E peptide in human prostate cancer cells. Prostate. 70:1233–1242. 2010. View Article : Google Scholar

31 

Philippou A, Armakolas A and Koutsilieris M: Evidence for the possible biological significance of the IGF-1 gene alternative splicing in prostate cancer. Front Endocrinol (Lausanne). 4:312013.PubMed/NCBI

32 

Straub L: Beyond the transcripts: what controls protein variation? PLoS Biol. 9:e10011462011. View Article : Google Scholar : PubMed/NCBI

33 

Vogel C and Marcotte EM: Insights into the regulation of protein abundance from proteomic and transcriptomic analyses. Nat Rev Genet. 13:227–232. 2012.PubMed/NCBI

34 

Klevebring D, Fagerberg L, Lundberg E, Emanuelsson O, Uhlén M and Lundeberg J: Analysis of transcript and protein overlap in a human osteosarcoma cell line. BMC Genomics. 11:6842010. View Article : Google Scholar : PubMed/NCBI

35 

Dengler MA, Weilbacher A, Gutekunst M, et al: Discrepant NOXA (PMAIP1) transcript and NOXA protein levels: a potential Achilles’ heel in mantle cell lymphoma. Cell Death Dis. 5:e10132014.PubMed/NCBI

36 

Mole S, McFarlane M, Chuen-Im T, Milligan SG, Millan D and Graham SV: RNA splicing factors regulated by HPV16 during cervical tumour progression. J Pathol. 219:383–391. 2009. View Article : Google Scholar : PubMed/NCBI

37 

Condemine W, Takahashi Y, Zhu J, et al: Characterization of endogenous human promyelocytic leukemia isoforms. Cancer Res. 66:6192–6198. 2006. View Article : Google Scholar : PubMed/NCBI

38 

Nojima T, Oshiro-Ideue T, Nakanoya H, et al: Herpesvirus protein ICP27 switches PML isoform by altering mRNA splicing. Nucleic Acids Res. 37:6515–6527. 2009. View Article : Google Scholar : PubMed/NCBI

39 

Liu Y, Tong Z, Li T, et al: Hepatitis B virus × protein stabilizes amplified in breast cancer 1 protein and cooperates with it to promote human hepatocellular carcinoma cell invasiveness. Hepatology. 56:1015–1024. 2012.

40 

Kalamvoki M and Roizman B: HSV-1 degrades, stabilizes, requires, or is stung by STING depending on ICP0, the US3 protein kinase, and cell derivation. Proc Natl Acad Sci USA. 111:E611–E617. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Durzyńska J and Barton E: IGF expression in HPV-related and HPV-unrelated human cancer cells. Oncol Rep 32: 893-900, 2014.
APA
Durzyńska, J., & Barton, E. (2014). IGF expression in HPV-related and HPV-unrelated human cancer cells. Oncology Reports, 32, 893-900. https://doi.org/10.3892/or.2014.3329
MLA
Durzyńska, J., Barton, E."IGF expression in HPV-related and HPV-unrelated human cancer cells". Oncology Reports 32.3 (2014): 893-900.
Chicago
Durzyńska, J., Barton, E."IGF expression in HPV-related and HPV-unrelated human cancer cells". Oncology Reports 32, no. 3 (2014): 893-900. https://doi.org/10.3892/or.2014.3329
Copy and paste a formatted citation
x
Spandidos Publications style
Durzyńska J and Barton E: IGF expression in HPV-related and HPV-unrelated human cancer cells. Oncol Rep 32: 893-900, 2014.
APA
Durzyńska, J., & Barton, E. (2014). IGF expression in HPV-related and HPV-unrelated human cancer cells. Oncology Reports, 32, 893-900. https://doi.org/10.3892/or.2014.3329
MLA
Durzyńska, J., Barton, E."IGF expression in HPV-related and HPV-unrelated human cancer cells". Oncology Reports 32.3 (2014): 893-900.
Chicago
Durzyńska, J., Barton, E."IGF expression in HPV-related and HPV-unrelated human cancer cells". Oncology Reports 32, no. 3 (2014): 893-900. https://doi.org/10.3892/or.2014.3329
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team