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Article

A comprehensive search for microRNAs with expression profiles modulated by oncogenic KRAS: Potential involvement of miR-31 in lung carcinogenesis

  • Authors:
    • Koji Okudela
    • Takeshisa Suzuki
    • Shigeaki  Umeda
    • Yoko  Tateishi
    • Hideaki  Mitsui
    • Yohei  Miyagi
    • Kenichi  Ohashi
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Yokohama City University, Graduate School of Medicine, Kanazawa-Ku, Yokohama 236-0004, Japan, Clinical Research Institute, Kanagawa Prefectural Cancer Center Hospital, Asahi-ku, Yokohama 241-8515, Japan
  • Pages: 1374-1384
    |
    Published online on: July 18, 2014
       https://doi.org/10.3892/or.2014.3339
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Abstract

Small non-protein coding RNAs that regulate messenger RNA levels, namely microRNAs (miRNAs), have been implicated in the pathogenesis of various diseases. The purpose of the present study was to identify essential miRNAs involved in lung carcinogenesis. Previous studies demonstrated that an investigation into the downstream targets of oncogenic KRAS could be used as a strategy to elucidate the molecular mechanisms involved in lung cancer; therefore, we examined the expression profiles of mRNAs modulated by oncogenic KRAS in the present study. We focused on miR-31 from the miRNAs that were differentially expressed, and evaluated its potential role in the development of lung cancer. miR-31 was upregulated not only by oncogenic KRAS, but also by oncogenic EGFR. The expression of miR-31 was markedly attenuated in some lung cancer cell lines by deleting its host gene locus. The restoration of miR-31 in lung cancer cell lines that lost its expression attenuated their growth activities. The knockdown of miR-31 expression in lung cancer cell lines retaining its expression enhanced anchorage-independent growth activity. These results suggest that miR-31 may be a suppressor that regulates an essential oncogenic pathway, the loss of which may promote lung carcinogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Okudela K, Suzuki T, Umeda S, Tateishi Y, Mitsui H, Miyagi Y and Ohashi K: A comprehensive search for microRNAs with expression profiles modulated by oncogenic KRAS: Potential involvement of miR-31 in lung carcinogenesis. Oncol Rep 32: 1374-1384, 2014.
APA
Okudela, K., Suzuki, T., Umeda, S., Tateishi, Y., Mitsui, H., Miyagi, Y., & Ohashi, K. (2014). A comprehensive search for microRNAs with expression profiles modulated by oncogenic KRAS: Potential involvement of miR-31 in lung carcinogenesis. Oncology Reports, 32, 1374-1384. https://doi.org/10.3892/or.2014.3339
MLA
Okudela, K., Suzuki, T., Umeda, S., Tateishi, Y., Mitsui, H., Miyagi, Y., Ohashi, K."A comprehensive search for microRNAs with expression profiles modulated by oncogenic KRAS: Potential involvement of miR-31 in lung carcinogenesis". Oncology Reports 32.4 (2014): 1374-1384.
Chicago
Okudela, K., Suzuki, T., Umeda, S., Tateishi, Y., Mitsui, H., Miyagi, Y., Ohashi, K."A comprehensive search for microRNAs with expression profiles modulated by oncogenic KRAS: Potential involvement of miR-31 in lung carcinogenesis". Oncology Reports 32, no. 4 (2014): 1374-1384. https://doi.org/10.3892/or.2014.3339
Copy and paste a formatted citation
x
Spandidos Publications style
Okudela K, Suzuki T, Umeda S, Tateishi Y, Mitsui H, Miyagi Y and Ohashi K: A comprehensive search for microRNAs with expression profiles modulated by oncogenic KRAS: Potential involvement of miR-31 in lung carcinogenesis. Oncol Rep 32: 1374-1384, 2014.
APA
Okudela, K., Suzuki, T., Umeda, S., Tateishi, Y., Mitsui, H., Miyagi, Y., & Ohashi, K. (2014). A comprehensive search for microRNAs with expression profiles modulated by oncogenic KRAS: Potential involvement of miR-31 in lung carcinogenesis. Oncology Reports, 32, 1374-1384. https://doi.org/10.3892/or.2014.3339
MLA
Okudela, K., Suzuki, T., Umeda, S., Tateishi, Y., Mitsui, H., Miyagi, Y., Ohashi, K."A comprehensive search for microRNAs with expression profiles modulated by oncogenic KRAS: Potential involvement of miR-31 in lung carcinogenesis". Oncology Reports 32.4 (2014): 1374-1384.
Chicago
Okudela, K., Suzuki, T., Umeda, S., Tateishi, Y., Mitsui, H., Miyagi, Y., Ohashi, K."A comprehensive search for microRNAs with expression profiles modulated by oncogenic KRAS: Potential involvement of miR-31 in lung carcinogenesis". Oncology Reports 32, no. 4 (2014): 1374-1384. https://doi.org/10.3892/or.2014.3339
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