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Article

Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors

  • Authors:
    • Rong-Feng Song
    • Xiao-Jun Li
    • Xiao-Liang Cheng
    • Ai-Rong  Fu
    • Yan-Hua Wang
    • Yan-Jun Feng
    • Yan Xiong
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Cancer Hospital of Jiangxi Province, Nanchang, Jiangxi 330029, P.R. China, Department of Hepatic Neoplasms Surgery, Cancer Hospital of Jiangxi Province, Nanchang, Jiangxi 330029, P.R. China, Department of Thoracic Surgery, Cancer Hospital of Jiangxi Province, Nanchang, Jiangxi 330029, P.R. China, Department of Pathology, Cancer Hospital of Jiangxi Province, Nanchang, Jiangxi 330029, P.R. China
  • Pages: 1481-1488
    |
    Published online on: July 21, 2014
       https://doi.org/10.3892/or.2014.3344
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Abstract

Gastroenteric cancer is one of the most prevalent cancers and is responsible for most cancer-related deaths worldwide. Paclitaxel (PTX), a classical microtubule inhibitor, is indicated in the treatment of gastric/gastroenteric cancers. In the present study, trimethyl chitosan (TMC)-loaded PTX (TMC-PTX) was prepared and evaluated for its therapeutic effect in gastric cancers. A spherical shaped nanosized TMC-PTX particle was formed with high loading capacity (~30%) for PTX. The nanoparticles (NPs) showed a sustained release pattern (~70%) for up to 96 h of study period. The positively charged NPs were preferentially internalized by Caco-2 cells. TMC-PTX inhibited the gastric cell proliferation with an IC50 value of 0.6 µg in NCI-N87 cells while it was 1.26 µg in the SGC-7901 cell line after 24 h exposure. The apoptosis assay (Annexin V/PI) showed a large presence of cells in the early and late apoptosis chamber, while cell cycle analysis showed a marked G2/M phase arrest (50-60%) in NCI-N87 and SGC-7901 cell lines indicating its potent anti-proliferative effect. The in vivo antitumor study in NCI-N87 and SGC-7901 bearing xenograft model showed a superior chemotherapeutic efficacy for TMC-PTX NP. The NP group significantly reduced the tumor growth with no obvious sign of systemic side-effects (safety). Collectively, our results suggest that the microtubule inhibitory effect of PTX-loaded polymer NP could be a promising system for the treatment of gastroenteric cancers.
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1 

Oh SC: Update of adjuvant chemotherapy for resected gastric cancer. J Gastric Cancer. 12:3–6. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Schwarz RE and Smith DD: Clinical impact of lymphadenectomy extent in resectable gastric cancer of advanced stage. Ann Surg Oncol. 14:317–328. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar

4 

Rowinsky EK, Cazenave LA and Donehower RC: Taxol: a novel investigational antimicrotubule agent. J Natl Cancer Inst. 82:1247–1259. 1990. View Article : Google Scholar : PubMed/NCBI

5 

Mancuso A, Oudard S and Sternberg CN: Effective chemotherapy for hormone-refractory prostate cancer (HRPC): present status and perspectives with taxane-based treatments. Crit Rev Oncol Hematol. 61:176–185. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Weiss RB, Donehower RC, Wiernik PH, Ohnuma T, Gralla RJ, Trump DL, et al: Hypersensitivity reactions from taxol. J Clin Oncol. 8:1263–1268. 1990.PubMed/NCBI

7 

Akhlaghi SP, Saremi S, Ostad SN, Dinarvand R and Atyabi F: Discriminated effects of thiolated chitosan-coated pMMA paclitaxel-loaded nanoparticles on different normal and cancer cell lines. Nanomedicine. 6:689–697. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Zhang W, Shi Y, Chen Y, Yu S, Hao J, Luo J, Sha X and Fang X: Enhanced antitumor efficacy by paclitaxel-loaded pluronic P123/F127 mixed micelles against non-small cell lung cancer based on passive tumor targeting and modulation of drug resistance. Eur J Pharm Biopharm. 75:341–353. 2010. View Article : Google Scholar : PubMed/NCBI

9 

de Britto D and Assis OBG: A novel method for obtaining a quaternary salt of chitosan. Carbohydr Polym. 69:305–310. 2007.

10 

Slütter B and Jiskoot W: Dual role of CpG as immune modulator and physical crosslinker in ovalbumin loaded N-trimethyl chitosan (TMC) nanoparticles for nasal vaccination. J Control Release. 148:117–121. 2010.PubMed/NCBI

11 

Subbiah R, Ramalingam P, Ramasundaram S, et al: N,N,N-trimethyl chitosan nanoparticles for controlled intranasal delivery of HBV surface antigen. Carbohydr Polym. 89:1289–1297. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Hamman JH, Stander M and Kotze AF: Effect of the degree of quaternisation of N-trimethyl chitosan chloride on absorption enhancement: in vivo evaluation in rat nasal epithelia. Int J Pharm. 232:235–242. 2002. View Article : Google Scholar : PubMed/NCBI

13 

de Britto D, Forato LA and Assis OBG: Determination of the average degree of quaternization of N,N,N-trimethylchitosan by solid state C-13 NMR. Carbohydr Polym. 74:86–91. 2008.

14 

de Moura MR, Aouada FA, Avena-Bustillos RJ, McHugh TH, Krochta JM and Mattoso LHC: Improved barrier and mechanical properties of novel hydroxypropyl methylcellulose edible films with chitosan/tripolyphosphate nanoparticles. J Food Eng. 92:448–453. 2009.

15 

de Britto D, de Moura MR, Aouada FA, Mattoso LHC and Assis OBG: N,N,N-trimethyl chitosan nanoparticles as a vitamin carrier system. Food Hydrocoll. 27:487–493. 2012.

16 

Bhumkar DR and Pokharkar VB: Studies on effect of pH on cross-linking of chitosan with sodium tripolyphosphate: a technical note. AAPS PharmSciTech. 7:E502006. View Article : Google Scholar : PubMed/NCBI

17 

Chen F, Zhang ZR and Huang Y: Evaluation and modification of N-trimethyl chitosan chloride nanoparticles as protein carriers. Int J Pharm. 336:166–173. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Gan Q and Wang T: Chitosan nanoparticle as protein delivery carrier - systematic examination of fabrication conditions for efficient loading and release. Colloids Surf B Biointerfaces. 59:24–34. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Qi L, Xu Z, Jiang X, Hu C and Zou X: Preparation and antibacterial activity of chitosan nanoparticles. Carbohydr Res. 339:2693–2700. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Davda J and Labhasetwar V: Characterization of nanoparticle uptake by endothelial cells. Int J Pharm. 233:51–59. 2002. View Article : Google Scholar

21 

Yu B, Zhang Y, Zheng W, Fan C and Chen T: Positive surface charge enhances selective cellular uptake and anticancer efficacy of selenium nanoparticles. Inorg Chem. 51:8956–8963. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Ganesh T, Yang C, Norris A, et al: Evaluation of the tubulin-bound paclitaxel conformation: synthesis, biology, and SAR studies of C-4 to C-3′ bridged paclitaxel analogues. J Med Chem. 50:713–725. 2007.PubMed/NCBI

23 

Sharma AK, Zhang L, Li S, Kelly DL, Alakhov VY, Batrakova EV and Kabanov AV: Prevention of MDR development in leukemia cells by micelle-forming polymeric surfactant. J Control Release. 131:220–227. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Guo DD, Moon HS, Arote R, Seo JH, Quan JS, Choi YJ and Cho CS: Enhanced anticancer effect of conjugated linoleic acid by conjugation with Pluronic F127 on MCF-7 breast cancer cells. Cancer Lett. 254:244–254. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Guo DD, Xu CX, Quan JS, Song CK, Jin H, Kim DD, Choi YJ, Cho MH and Cho CS: Synergistic anti-tumor activity of paclitaxel-incorporated conjugated linoleic acid-coupled poloxamer thermosensitive hydrogel in vitro and in vivo. Biomaterials. 30:4777–4785. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Ahmed F, Pakunlu RI, Brannan A, Bates F, Minko T and Discher DE: Biodegradable polymersomes loaded with both paclitaxel and doxorubicin permeate and shrink tumors, inducing apoptosis in proportion to accumulated drug. J Control Release. 116:150–158. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Ng SSW, Tsao MS, Chow S and Hedley DW: Inhibition of phosphatidylinositide 3-kinase enhances gemcitabine-induced apoptosis in human pancreatic cancer cells. Cancer Res. 60:5451–5455. 2000.PubMed/NCBI

28 

Kim K, Kim JH, Park H, Kim YS, Park K, Nam H, et al: Tumor-homing multifunctional nanoparticles for cancer theragnosis: simultaneous diagnosis, drug delivery, and therapeutic monitoring. J Control Release. 146:219–227. 2010. View Article : Google Scholar

29 

Zhang L, He Y, Ma G, Song C and Sun H: Paclitaxel-loaded polymeric micelles based on poly(ɛ-caprolactone)-poly(ethylene glycol)-poly(ɛ-caprolactone) triblock copolymers: in vitro and in vivo evaluation. Nanomedicine. 8:925–934. 2012.

30 

Xiao K, Luo J, Fowler WL, Li Y, Lee JS, Xing L, Cheng RH, Wang L and Lam KS: A self-assembling nanoparticle for paclitaxel delivery in ovarian cancer. Biomaterials. 30:6006–6016. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Song R, Li X, Cheng X, Fu A, Wang Y, Feng Y and Xiong Y: Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors. Oncol Rep 32: 1481-1488, 2014.
APA
Song, R., Li, X., Cheng, X., Fu, A., Wang, Y., Feng, Y., & Xiong, Y. (2014). Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors. Oncology Reports, 32, 1481-1488. https://doi.org/10.3892/or.2014.3344
MLA
Song, R., Li, X., Cheng, X., Fu, A., Wang, Y., Feng, Y., Xiong, Y."Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors". Oncology Reports 32.4 (2014): 1481-1488.
Chicago
Song, R., Li, X., Cheng, X., Fu, A., Wang, Y., Feng, Y., Xiong, Y."Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors". Oncology Reports 32, no. 4 (2014): 1481-1488. https://doi.org/10.3892/or.2014.3344
Copy and paste a formatted citation
x
Spandidos Publications style
Song R, Li X, Cheng X, Fu A, Wang Y, Feng Y and Xiong Y: Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors. Oncol Rep 32: 1481-1488, 2014.
APA
Song, R., Li, X., Cheng, X., Fu, A., Wang, Y., Feng, Y., & Xiong, Y. (2014). Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors. Oncology Reports, 32, 1481-1488. https://doi.org/10.3892/or.2014.3344
MLA
Song, R., Li, X., Cheng, X., Fu, A., Wang, Y., Feng, Y., Xiong, Y."Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors". Oncology Reports 32.4 (2014): 1481-1488.
Chicago
Song, R., Li, X., Cheng, X., Fu, A., Wang, Y., Feng, Y., Xiong, Y."Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors". Oncology Reports 32, no. 4 (2014): 1481-1488. https://doi.org/10.3892/or.2014.3344
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