Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors

  • Authors:
    • Rong-Feng Song
    • Xiao-Jun Li
    • Xiao-Liang Cheng
    • Ai-Rong  Fu
    • Yan-Hua Wang
    • Yan-Jun Feng
    • Yan Xiong
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  • Published online on: July 21, 2014     https://doi.org/10.3892/or.2014.3344
  • Pages: 1481-1488
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Abstract

Gastroenteric cancer is one of the most prevalent cancers and is responsible for most cancer-related deaths worldwide. Paclitaxel (PTX), a classical microtubule inhibitor, is indicated in the treatment of gastric/gastroenteric cancers. In the present study, trimethyl chitosan (TMC)-loaded PTX (TMC-PTX) was prepared and evaluated for its therapeutic effect in gastric cancers. A spherical shaped nanosized TMC-PTX particle was formed with high loading capacity (~30%) for PTX. The nanoparticles (NPs) showed a sustained release pattern (~70%) for up to 96 h of study period. The positively charged NPs were preferentially internalized by Caco-2 cells. TMC-PTX inhibited the gastric cell proliferation with an IC50 value of 0.6 µg in NCI-N87 cells while it was 1.26 µg in the SGC-7901 cell line after 24 h exposure. The apoptosis assay (Annexin V/PI) showed a large presence of cells in the early and late apoptosis chamber, while cell cycle analysis showed a marked G2/M phase arrest (50-60%) in NCI-N87 and SGC-7901 cell lines indicating its potent anti-proliferative effect. The in vivo antitumor study in NCI-N87 and SGC-7901 bearing xenograft model showed a superior chemotherapeutic efficacy for TMC-PTX NP. The NP group significantly reduced the tumor growth with no obvious sign of systemic side-effects (safety). Collectively, our results suggest that the microtubule inhibitory effect of PTX-loaded polymer NP could be a promising system for the treatment of gastroenteric cancers.
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October 2014
Volume 32 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Song R, Li X, Cheng X, Fu A, Wang Y, Feng Y and Xiong Y: Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors. Oncol Rep 32: 1481-1488, 2014
APA
Song, R., Li, X., Cheng, X., Fu, A., Wang, Y., Feng, Y., & Xiong, Y. (2014). Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors. Oncology Reports, 32, 1481-1488. https://doi.org/10.3892/or.2014.3344
MLA
Song, R., Li, X., Cheng, X., Fu, A., Wang, Y., Feng, Y., Xiong, Y."Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors". Oncology Reports 32.4 (2014): 1481-1488.
Chicago
Song, R., Li, X., Cheng, X., Fu, A., Wang, Y., Feng, Y., Xiong, Y."Paclitaxel-loaded trimethyl chitosan-based polymeric nanoparticle for the effective treatment of gastroenteric tumors". Oncology Reports 32, no. 4 (2014): 1481-1488. https://doi.org/10.3892/or.2014.3344