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October 2014 Volume 32 Issue 4

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Article

Diallyl disulfide induces G2/M arrest and promotes apoptosis through the p53/p21 and MEK-ERK pathways in human esophageal squamous cell carcinoma

  • Authors:
    • Xiaoran Yin
    • Rong Zhang
    • Cheng Feng
    • Jun Zhang
    • Dong Liu
    • Kun Xu
    • Xijing Wang
    • Shuqun Zhang
    • Zongfang Li
    • Xinlian Liu
    • Hongbing Ma
  • View Affiliations

    Affiliations: Department of Oncology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Gastroenterology, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China, Department of Digestion, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of General Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
  • Published online on: July 25, 2014     https://doi.org/10.3892/or.2014.3361
  • Pages: 1748-1756
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Abstract

Esophageal squamous cell carcinoma (ESCC) is an aggressive tumor with high incidence and mortality worldwide. Diallyl disulfide (DADS) is a natural organosulfur compound, isolated from garlic. In this study, MTT assay showed that DADS significantly reduced cell viability in a dose- and time-dependent manner in ESCC cells, with lower toxicity in normal liver cells. Cell cycle analysis revealed that DADS made G2/M phase arrest. Molecular analysis suggested that this cell cycle arrest was likely made by the decrease of cyclin B1, cdc2, p-cdc2, cdc25c in concomitance with activation of the p53/p21 pathway. Apoptosis was detected by Annexin V/PI staining. The molecule markers showed that DADS induced apoptosis through activating caspases, altering the Bax/Bcl-2 balance and suppressing the MEK-ERK pathway. Our data indicated that DADS has the potential to be an effective and safe anticancer agent for ESCC therapy in the near future.
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Copy and paste a formatted citation
Spandidos Publications style
Yin X, Zhang R, Feng C, Zhang J, Liu D, Xu K, Wang X, Zhang S, Li Z, Liu X, Liu X, et al: Diallyl disulfide induces G2/M arrest and promotes apoptosis through the p53/p21 and MEK-ERK pathways in human esophageal squamous cell carcinoma. Oncol Rep 32: 1748-1756, 2014.
APA
Yin, X., Zhang, R., Feng, C., Zhang, J., Liu, D., Xu, K. ... Ma, H. (2014). Diallyl disulfide induces G2/M arrest and promotes apoptosis through the p53/p21 and MEK-ERK pathways in human esophageal squamous cell carcinoma. Oncology Reports, 32, 1748-1756. https://doi.org/10.3892/or.2014.3361
MLA
Yin, X., Zhang, R., Feng, C., Zhang, J., Liu, D., Xu, K., Wang, X., Zhang, S., Li, Z., Liu, X., Ma, H."Diallyl disulfide induces G2/M arrest and promotes apoptosis through the p53/p21 and MEK-ERK pathways in human esophageal squamous cell carcinoma". Oncology Reports 32.4 (2014): 1748-1756.
Chicago
Yin, X., Zhang, R., Feng, C., Zhang, J., Liu, D., Xu, K., Wang, X., Zhang, S., Li, Z., Liu, X., Ma, H."Diallyl disulfide induces G2/M arrest and promotes apoptosis through the p53/p21 and MEK-ERK pathways in human esophageal squamous cell carcinoma". Oncology Reports 32, no. 4 (2014): 1748-1756. https://doi.org/10.3892/or.2014.3361
Copy and paste a formatted citation
x
Spandidos Publications style
Yin X, Zhang R, Feng C, Zhang J, Liu D, Xu K, Wang X, Zhang S, Li Z, Liu X, Liu X, et al: Diallyl disulfide induces G2/M arrest and promotes apoptosis through the p53/p21 and MEK-ERK pathways in human esophageal squamous cell carcinoma. Oncol Rep 32: 1748-1756, 2014.
APA
Yin, X., Zhang, R., Feng, C., Zhang, J., Liu, D., Xu, K. ... Ma, H. (2014). Diallyl disulfide induces G2/M arrest and promotes apoptosis through the p53/p21 and MEK-ERK pathways in human esophageal squamous cell carcinoma. Oncology Reports, 32, 1748-1756. https://doi.org/10.3892/or.2014.3361
MLA
Yin, X., Zhang, R., Feng, C., Zhang, J., Liu, D., Xu, K., Wang, X., Zhang, S., Li, Z., Liu, X., Ma, H."Diallyl disulfide induces G2/M arrest and promotes apoptosis through the p53/p21 and MEK-ERK pathways in human esophageal squamous cell carcinoma". Oncology Reports 32.4 (2014): 1748-1756.
Chicago
Yin, X., Zhang, R., Feng, C., Zhang, J., Liu, D., Xu, K., Wang, X., Zhang, S., Li, Z., Liu, X., Ma, H."Diallyl disulfide induces G2/M arrest and promotes apoptosis through the p53/p21 and MEK-ERK pathways in human esophageal squamous cell carcinoma". Oncology Reports 32, no. 4 (2014): 1748-1756. https://doi.org/10.3892/or.2014.3361
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