Effect of the PTEN gene on adhesion, invasion and metastasis of osteosarcoma cells

  • Authors:
    • Yong Hu
    • Shenglin Xu
    • Wensen Jin
    • Qiyi Yi
    • Wei  Wei
  • View Affiliations

  • Published online on: July 25, 2014     https://doi.org/10.3892/or.2014.3362
  • Pages: 1741-1747
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Abstract

The phosphatase and tensin homolog (PTEN) gene, an important tumor-suppressor gene, has been demonstrated to have the potential for inhibiting proliferation, migration and invasion in various types of cancer cells. The aim of the present study was to investigate the effect of PTEN expression on osteosarcoma (OS) cells. The wild-type PTEN plasmid was transfected into OS U2-OS cells. The effects of PTEN on the adhesion, migration and invasion of U2-OS cells were evaluated by cell adhesion analysis, in vitro scratch and Transwell assays, respectively. The levels of MMP-2 and MMP-9, and focal adhesion kinase (FAK) protein regulated by PTEN were detected via western blot analysis. Meanwhile, the level of intracellular FAK phosphorylation was observed. The results from the present study showed that overexpression of PTEN transcription and protein were observed in U2-OS cells following PTEN transfection. Furthermore, the migration, invasion and adhesion capabilities of the cells with PTEN transfection were significantly decreased compared to these capacities in the cells without PTEN. Meanwhile, it was shown that there was downregulation of MMP-9, FAK and p-FAK concomitant with the elevation of the intracellular PTEN level. It is therefore evident that the upregulation of PTEN may attenuate the adhesion, migration and invasion capabilities of OS cells. The mechanisms of the effects of PTEN on OS cells may be correlated with a reduction in the related genes by PTEN regulation.
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October 2014
Volume 32 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Hu Y, Xu S, Jin W, Yi Q and Wei W: Effect of the PTEN gene on adhesion, invasion and metastasis of osteosarcoma cells. Oncol Rep 32: 1741-1747, 2014
APA
Hu, Y., Xu, S., Jin, W., Yi, Q., & Wei, W. (2014). Effect of the PTEN gene on adhesion, invasion and metastasis of osteosarcoma cells. Oncology Reports, 32, 1741-1747. https://doi.org/10.3892/or.2014.3362
MLA
Hu, Y., Xu, S., Jin, W., Yi, Q., Wei, W."Effect of the PTEN gene on adhesion, invasion and metastasis of osteosarcoma cells". Oncology Reports 32.4 (2014): 1741-1747.
Chicago
Hu, Y., Xu, S., Jin, W., Yi, Q., Wei, W."Effect of the PTEN gene on adhesion, invasion and metastasis of osteosarcoma cells". Oncology Reports 32, no. 4 (2014): 1741-1747. https://doi.org/10.3892/or.2014.3362