Synergistic cytotoxic effect of genistein and doxorubicin on drug-resistant human breast cancer MCF-7/Adr cells

  • Authors:
    • Jia-Peng Xue
    • Geng Wang
    • Zong-Bin Zhao
    • Qun Wang
    • Yun Shi
  • View Affiliations

  • Published online on: July 29, 2014     https://doi.org/10.3892/or.2014.3365
  • Pages: 1647-1653
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Abstract

The molecular mechanisms underlying genistein-mediated reversal of chemoresistance remains unknown. In the present study, we investigated the molecular mechanisms by which genistein overcomes chemoresistance and its effect on doxorubicin-induced cytotoxicity. Consistent with previous reports, genistein combined with doxorubicin had a synergistic effect on MCF-7/Adr cells, and genistein reduced the chemoresistance of these cells. Genistein treatment increased the intracellular accumulation of doxorubicin but did not influence P-gp function. The combination of genistein and doxorubicin significantly induced cell cycle arrest and apoptosis. Genistein treatment strongly inhibited HER2/neu but not MDR-1 expression at both the mRNA and protein levels. Therefore, our results demonstrated that genistein combined with doxorubicin had a synergistic effect on MCF-7/Adr cells, and the mechanisms likely involve an increase in the intracellular accumulation of doxorubicin and suppression of HER2/neu expression.
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October 2014
Volume 32 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xue J, Wang G, Zhao Z, Wang Q and Shi Y: Synergistic cytotoxic effect of genistein and doxorubicin on drug-resistant human breast cancer MCF-7/Adr cells. Oncol Rep 32: 1647-1653, 2014
APA
Xue, J., Wang, G., Zhao, Z., Wang, Q., & Shi, Y. (2014). Synergistic cytotoxic effect of genistein and doxorubicin on drug-resistant human breast cancer MCF-7/Adr cells. Oncology Reports, 32, 1647-1653. https://doi.org/10.3892/or.2014.3365
MLA
Xue, J., Wang, G., Zhao, Z., Wang, Q., Shi, Y."Synergistic cytotoxic effect of genistein and doxorubicin on drug-resistant human breast cancer MCF-7/Adr cells". Oncology Reports 32.4 (2014): 1647-1653.
Chicago
Xue, J., Wang, G., Zhao, Z., Wang, Q., Shi, Y."Synergistic cytotoxic effect of genistein and doxorubicin on drug-resistant human breast cancer MCF-7/Adr cells". Oncology Reports 32, no. 4 (2014): 1647-1653. https://doi.org/10.3892/or.2014.3365