Aberrant expression of B7-H3 in gastric adenocarcinoma promotes cancer cell metastasis Retraction in /10.3892/or.2022.8421

Dai,Wei; Shen,Genhai; Qiu,Jianping; Zhao,Xin; Gao,Quangen

doi:10.3892/or.2014.3405

Aberrant expression of B7-H3 in gastric adenocarcinoma promotes cancer cell metastasis

Retraction in: /10.3892/or.2022.8421

Authors:
- Wei Dai
- Genhai Shen
- Jianping Qiu
- Xin Zhao
- Quangen Gao
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Affiliations: Department of General Surgery, The First People's Hospital of Wujiang, Wujiang, Jiangsu 215200, P.R. China, Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
Published online on: August 14, 2014 https://doi.org/10.3892/or.2014.3405
Pages: 2086-2092

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Abstract

B7-H3 belongs to the B7 superfamily, a group of molecules that costimulate or downmodulate T cell responses. Although it has been shown that B7-H3 can inhibit T cell responses, several studies, most of them performed in murine systems, found B7-H3 to act in a co-stimulatory manner. In addition, B7-H3 is also expressed in various human cancers and is correlated with the poor outcome of cancer patients. The functional role of B7-H3 in cancer is still controversially discussed. In the present study, we compared B7-H3 expression in normal gastric tissues and gastric cancer tissue specimens and determined the effects of low B7-H3 expression on the human gastric cancer cell line SGC-7901 by using RNAi. B7-H3 expression in gastric specimens was determined by tissue qPCR and immunohistochemisty. A SGC-7901 cell line with low B7-H3 expression was established by lentiviral-mediated RNA interference to investigate the effect of B7-H3 on cancer cell migration and invasion in vitro. By establishing an orthotopic transplantation gastric cancer mouse model, the effect of B7-H3 on cell migration and invasion was studied in vivo. B7-H3 expression was significantly higher in the gastric cancer group than that in the normal gaster group. B7-H3 knockdown by RNA interference decreased cell migration and Transwell invasion up to 50% in vitro. In the orthotopic transplantation gastric cancer mouse model, the effect of inhibiting metastasis by knockdown of B7-H3 was assessed in terms of the average postmortem abdominal visceral metastatic tumor weight. The results revealed that inhibition of B7-H3 expression reduced gastric cancer metastasis in vivo. In conclusion, B7-H3 is aberrantly expressed in gastric cancer. In addition to modulating tumor immunity, B7-H3 may have a novel role in regulating SGC-7901 cell metastasis.

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