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Article

MicroRNA-145 inhibits cell proliferation by directly targeting ADAM17 in hepatocellular carcinoma

  • Authors:
    • Yuwu Liu
    • Chang Wu
    • Ying Wang
    • Sailan Wen
    • Junpu Wang
    • Zhihong Chen
    • Qiongqiong He
    • Deyun Feng
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Pathology, Shenzhen Sixth People's Hospital (Nanshan Hospital), Shenzhen, Guangdong 518052, P.R. China
  • Pages: 1923-1930
    |
    Published online on: August 20, 2014
       https://doi.org/10.3892/or.2014.3424
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Abstract

MicroRNAs (miRNAs) are key regulators in cell processes. Emerging evidence has suggested that there is a direct association between miRNAs and cancer. However, the exact regulatory mechanisms of miRNAs in tumorigenesis are poorly understood. In the present study, we showed that miR-145 is able to significantly reduce mRNA and protein expression levels of A disintegrin and metalloproteinase 17 (ADAM17) in liver cancer cells (SMMC-7721, BEL-7402 and Huh-7). Dual luciferase reporter assays confirmed that ADAM17 is a direct target of miR-145. Notably, we found that miR-145 inhibits cell proliferation and growth activity in SMMC-7721 cells. These results demonstrated that it may be exert the function of tumor suppression in a particular link of cancer cell growth. Further studies revealed that the silencing of ADAM17 decreased the proliferation and growth activity of SMMC-7721 cells. Moreover, it reduced the expression of MMP-9. In conclusion, miR-145 inhibits liver cancer cell proliferation by directly targeting ADAM17. Thus, it may become a promising biological target in the treatment strategy of hepatocellular carcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Wu C, Wang Y, Wen S, Wang J, Chen Z, He Q and Feng D: MicroRNA-145 inhibits cell proliferation by directly targeting ADAM17 in hepatocellular carcinoma. Oncol Rep 32: 1923-1930, 2014.
APA
Liu, Y., Wu, C., Wang, Y., Wen, S., Wang, J., Chen, Z. ... Feng, D. (2014). MicroRNA-145 inhibits cell proliferation by directly targeting ADAM17 in hepatocellular carcinoma. Oncology Reports, 32, 1923-1930. https://doi.org/10.3892/or.2014.3424
MLA
Liu, Y., Wu, C., Wang, Y., Wen, S., Wang, J., Chen, Z., He, Q., Feng, D."MicroRNA-145 inhibits cell proliferation by directly targeting ADAM17 in hepatocellular carcinoma". Oncology Reports 32.5 (2014): 1923-1930.
Chicago
Liu, Y., Wu, C., Wang, Y., Wen, S., Wang, J., Chen, Z., He, Q., Feng, D."MicroRNA-145 inhibits cell proliferation by directly targeting ADAM17 in hepatocellular carcinoma". Oncology Reports 32, no. 5 (2014): 1923-1930. https://doi.org/10.3892/or.2014.3424
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Wu C, Wang Y, Wen S, Wang J, Chen Z, He Q and Feng D: MicroRNA-145 inhibits cell proliferation by directly targeting ADAM17 in hepatocellular carcinoma. Oncol Rep 32: 1923-1930, 2014.
APA
Liu, Y., Wu, C., Wang, Y., Wen, S., Wang, J., Chen, Z. ... Feng, D. (2014). MicroRNA-145 inhibits cell proliferation by directly targeting ADAM17 in hepatocellular carcinoma. Oncology Reports, 32, 1923-1930. https://doi.org/10.3892/or.2014.3424
MLA
Liu, Y., Wu, C., Wang, Y., Wen, S., Wang, J., Chen, Z., He, Q., Feng, D."MicroRNA-145 inhibits cell proliferation by directly targeting ADAM17 in hepatocellular carcinoma". Oncology Reports 32.5 (2014): 1923-1930.
Chicago
Liu, Y., Wu, C., Wang, Y., Wen, S., Wang, J., Chen, Z., He, Q., Feng, D."MicroRNA-145 inhibits cell proliferation by directly targeting ADAM17 in hepatocellular carcinoma". Oncology Reports 32, no. 5 (2014): 1923-1930. https://doi.org/10.3892/or.2014.3424
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