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November-2014 Volume 32 Issue 5

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Article

The endogenous oxindole isatin induces apoptosis of MCF‑7 breast cancer cells through a mitochondrial pathway

  • Authors:
    • Zhongliang Ma
    • Lin Hou
    • Yuhong Jiang
    • Yanpin Chen
    • Jinlian Song
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China, Department of Biochemistry, Medical College, Qingdao University, Qingdao, Shandong 266021, P.R. China, Department of Laboratory, Women and Children's Hospital of Qingdao, Qingdao, Shandong 266011, P.R. China
  • Pages: 2111-2117
    |
    Published online on: August 20, 2014
       https://doi.org/10.3892/or.2014.3426
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Abstract

Isatin is an endogenous indole in mammalian tissues and fluids that is expected to have antitumor effects in human breast cancer cells. Human breast cancer cells (MCF-7) were exposed to isatin at various concentrations (0, 50, 100, 200 µmol/l) for 48 h. Apoptotic features were demonstrated by nuclei staining with Hoechst 33258 and flow cytometry. Bcl-2 and Bax mRNA were analyzed via reverse transcription-polymerase chain reaction. Bcl-2, Bax, the inhibitor of caspase-activated DNase (ICAD), and cytochrome c protein were analyzed by western blot analysis. Apoptosis, caspase-9 and -3 activation and mitochondrial depolarization were assayed by flow cytometry. The results showed that isatin induced apoptosis of MCF-7 cells. Furthermore, Bcl-2 expression was decreased and the ratio of Bcl-2 to Bax was significantly decreased by isatin. The mitochondrial transmembrane potential was markedly decreased and the release of cytochrome c into the cytosol was elevated following treatment with isatin. At the same time, caspase-9 and -3 were stimulated, followed by the degradation of ICAD, a caspase-3 substrate.
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Copy and paste a formatted citation
Spandidos Publications style
Ma Z, Hou L, Jiang Y, Chen Y and Song J: The endogenous oxindole isatin induces apoptosis of MCF‑7 breast cancer cells through a mitochondrial pathway. Oncol Rep 32: 2111-2117, 2014.
APA
Ma, Z., Hou, L., Jiang, Y., Chen, Y., & Song, J. (2014). The endogenous oxindole isatin induces apoptosis of MCF‑7 breast cancer cells through a mitochondrial pathway. Oncology Reports, 32, 2111-2117. https://doi.org/10.3892/or.2014.3426
MLA
Ma, Z., Hou, L., Jiang, Y., Chen, Y., Song, J."The endogenous oxindole isatin induces apoptosis of MCF‑7 breast cancer cells through a mitochondrial pathway". Oncology Reports 32.5 (2014): 2111-2117.
Chicago
Ma, Z., Hou, L., Jiang, Y., Chen, Y., Song, J."The endogenous oxindole isatin induces apoptosis of MCF‑7 breast cancer cells through a mitochondrial pathway". Oncology Reports 32, no. 5 (2014): 2111-2117. https://doi.org/10.3892/or.2014.3426
Copy and paste a formatted citation
x
Spandidos Publications style
Ma Z, Hou L, Jiang Y, Chen Y and Song J: The endogenous oxindole isatin induces apoptosis of MCF‑7 breast cancer cells through a mitochondrial pathway. Oncol Rep 32: 2111-2117, 2014.
APA
Ma, Z., Hou, L., Jiang, Y., Chen, Y., & Song, J. (2014). The endogenous oxindole isatin induces apoptosis of MCF‑7 breast cancer cells through a mitochondrial pathway. Oncology Reports, 32, 2111-2117. https://doi.org/10.3892/or.2014.3426
MLA
Ma, Z., Hou, L., Jiang, Y., Chen, Y., Song, J."The endogenous oxindole isatin induces apoptosis of MCF‑7 breast cancer cells through a mitochondrial pathway". Oncology Reports 32.5 (2014): 2111-2117.
Chicago
Ma, Z., Hou, L., Jiang, Y., Chen, Y., Song, J."The endogenous oxindole isatin induces apoptosis of MCF‑7 breast cancer cells through a mitochondrial pathway". Oncology Reports 32, no. 5 (2014): 2111-2117. https://doi.org/10.3892/or.2014.3426
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