Association of increased levels of TGF-β1 and p14ARF in prostate carcinoma cell lines overexpressing Egr-1

  • Authors:
    • Eduardo Parra
    • Luis Gutiérrez
    • Jorge Ferreira
  • View Affiliations

  • Published online on: September 9, 2014     https://doi.org/10.3892/or.2014.3472
  • Pages: 2191-2198
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Abstract

The present study examined the effect of the overexpression of early growth response gene (Egr-1) on transforming growth factor β-1 (TGF-β1) and p14ARF levels, in PC-3 and LNCaP prostate carcinoma cell lines. Amplification of EGR-1, TGF-β1 and p14ARF were observed in the two cell lines treated with different stimuli and resulted in a corresponding mRNA and protein expression. The downregulation of TGF-β1 and the attenuation of p14ARF expression by siRNA against Egr-1 predominantly suggested that TGF-β1 and p14ARF may be regulated by the transcription factor EGR-1. A marginal attenuation of cell growth in PC-3 and LNCaP prostate carcinoma cell lines overexpressing p14ARF was observed. Cells transfected with Egr-1 wild-type were able to grow and avoid cell cycle arrest and apoptosis in the presence or absence of p14ARF. In addition, EGR-1 stimulated the expression of TGF β-l as well as the accumulation of the p14ARF proteins. The results suggested that TGF-β1 and p14ARF activities in the presence of EGR-1 overexpression can exist independently of the presence of cells carrying a mutant p53 (PC-3 cells) or cells carrying a wild‑type p53 (LNCaP cells). Thus, the effect of EGR-1 on the growth of prostate carcinoma cells may occur through multiple mechanisms, but be independent of p53 expression control.
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November-2014
Volume 32 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Parra E, Gutiérrez L and Ferreira J: Association of increased levels of TGF-β1 and p14ARF in prostate carcinoma cell lines overexpressing Egr-1. Oncol Rep 32: 2191-2198, 2014
APA
Parra, E., Gutiérrez, L., & Ferreira, J. (2014). Association of increased levels of TGF-β1 and p14ARF in prostate carcinoma cell lines overexpressing Egr-1. Oncology Reports, 32, 2191-2198. https://doi.org/10.3892/or.2014.3472
MLA
Parra, E., Gutiérrez, L., Ferreira, J."Association of increased levels of TGF-β1 and p14ARF in prostate carcinoma cell lines overexpressing Egr-1". Oncology Reports 32.5 (2014): 2191-2198.
Chicago
Parra, E., Gutiérrez, L., Ferreira, J."Association of increased levels of TGF-β1 and p14ARF in prostate carcinoma cell lines overexpressing Egr-1". Oncology Reports 32, no. 5 (2014): 2191-2198. https://doi.org/10.3892/or.2014.3472