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Article

Association of increased levels of TGF-β1 and p14ARF in prostate carcinoma cell lines overexpressing Egr-1

  • Authors:
    • Eduardo Parra
    • Luis Gutiérrez
    • Jorge Ferreira
  • View Affiliations / Copyright

    Affiliations: Laboratory of Experimental Biomedicine, University of Tarapaca, Campus Esmeralda, Iquique, Chile, Faculty of Sciences, Arturo Prat University, Iquique, Chile, Programme of Molecular and Clinical Pharmacology, ICBM, Medical Faculty, University of Chile, Independencia, Santiago, Chile
  • Pages: 2191-2198
    |
    Published online on: September 9, 2014
       https://doi.org/10.3892/or.2014.3472
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Abstract

The present study examined the effect of the overexpression of early growth response gene (Egr-1) on transforming growth factor β-1 (TGF-β1) and p14ARF levels, in PC-3 and LNCaP prostate carcinoma cell lines. Amplification of EGR-1, TGF-β1 and p14ARF were observed in the two cell lines treated with different stimuli and resulted in a corresponding mRNA and protein expression. The downregulation of TGF-β1 and the attenuation of p14ARF expression by siRNA against Egr-1 predominantly suggested that TGF-β1 and p14ARF may be regulated by the transcription factor EGR-1. A marginal attenuation of cell growth in PC-3 and LNCaP prostate carcinoma cell lines overexpressing p14ARF was observed. Cells transfected with Egr-1 wild-type were able to grow and avoid cell cycle arrest and apoptosis in the presence or absence of p14ARF. In addition, EGR-1 stimulated the expression of TGF β-l as well as the accumulation of the p14ARF proteins. The results suggested that TGF-β1 and p14ARF activities in the presence of EGR-1 overexpression can exist independently of the presence of cells carrying a mutant p53 (PC-3 cells) or cells carrying a wild‑type p53 (LNCaP cells). Thus, the effect of EGR-1 on the growth of prostate carcinoma cells may occur through multiple mechanisms, but be independent of p53 expression control.
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Copy and paste a formatted citation
Spandidos Publications style
Parra E, Gutiérrez L and Ferreira J: Association of increased levels of TGF-β1 and p14ARF in prostate carcinoma cell lines overexpressing Egr-1. Oncol Rep 32: 2191-2198, 2014.
APA
Parra, E., Gutiérrez, L., & Ferreira, J. (2014). Association of increased levels of TGF-β1 and p14ARF in prostate carcinoma cell lines overexpressing Egr-1. Oncology Reports, 32, 2191-2198. https://doi.org/10.3892/or.2014.3472
MLA
Parra, E., Gutiérrez, L., Ferreira, J."Association of increased levels of TGF-β1 and p14ARF in prostate carcinoma cell lines overexpressing Egr-1". Oncology Reports 32.5 (2014): 2191-2198.
Chicago
Parra, E., Gutiérrez, L., Ferreira, J."Association of increased levels of TGF-β1 and p14ARF in prostate carcinoma cell lines overexpressing Egr-1". Oncology Reports 32, no. 5 (2014): 2191-2198. https://doi.org/10.3892/or.2014.3472
Copy and paste a formatted citation
x
Spandidos Publications style
Parra E, Gutiérrez L and Ferreira J: Association of increased levels of TGF-β1 and p14ARF in prostate carcinoma cell lines overexpressing Egr-1. Oncol Rep 32: 2191-2198, 2014.
APA
Parra, E., Gutiérrez, L., & Ferreira, J. (2014). Association of increased levels of TGF-β1 and p14ARF in prostate carcinoma cell lines overexpressing Egr-1. Oncology Reports, 32, 2191-2198. https://doi.org/10.3892/or.2014.3472
MLA
Parra, E., Gutiérrez, L., Ferreira, J."Association of increased levels of TGF-β1 and p14ARF in prostate carcinoma cell lines overexpressing Egr-1". Oncology Reports 32.5 (2014): 2191-2198.
Chicago
Parra, E., Gutiérrez, L., Ferreira, J."Association of increased levels of TGF-β1 and p14ARF in prostate carcinoma cell lines overexpressing Egr-1". Oncology Reports 32, no. 5 (2014): 2191-2198. https://doi.org/10.3892/or.2014.3472
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