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Article

EphA3, induced by PC-1/PrLZ, contributes to the malignant progression of prostate cancer

  • Authors:
    • Ruiqin Wu
    • Hongtao Wang
    • Jian Wang
    • Peng Wang
    • Fang Huang
    • Bangxiang Xie
    • Yali Zhao
    • Shanhu Li
    • Jianguang Zhou
  • View Affiliations / Copyright

    Affiliations: Laboratory of Medical Molecular Biology, Beijing Institute of Biotechnology, Beijing 100850, P.R. China
  • Pages: 2657-2665
    |
    Published online on: September 16, 2014
       https://doi.org/10.3892/or.2014.3482
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Abstract

Our previous study revealed the potential linkage of PC-1/PrLZ, a novel isolated prostate-specific gene, to the progression of prostate cancer (PCa) in vitro and in vivo. To gain more insight into the mechanism of PC-1-induced promotion of PCa, expression analysis of differential genes induced by PC-1 was scanned by microarray. Among all the differentially expressed genes, EphA3 was altered to the greatest extent. EphA3 has been identified to be associated with multiple tumor progression. However, little is known concerning the function of EphA3 in PCa. In the present study, we aimed to ascertain whether EphA3 is induced by PC-1 and the functional significance of EphA3 expression in PCa. We found that overexpression of PC-1 increased the amount of EphA3 and that knockdown of PC-1 led to a decrease in EphA3 in PCa cells. The functional significance and mechanisms by which EphA3 contributes to PCa was investigated in vitro using cell lines, and in vivo using a mouse model and clinical specimens. The results showed that EphA3 enhanced the proliferation and survival of LNCaP cells and suppression of EphA3 inhibited the survival of C4-2B cells. EphA3 enhanced the tumor development of LNCaP cells in null mice. A positive correlation between the levels of EphA3 and the Gleason grade of PCa was identified in clinical PCa specimens. In addition, cellular localization changed with Gleason grade. We further detected that EphA3 increased phosphorylation of Akt (Ser473 and Thr308), indicating that EphA3 activated the Akt pathway. Taken together, EphA3 was induced by PC-1 and contributed to the malignant progression of prostate cancer. Our results provide the first demonstration that EphA3 is a novel promoter of human prostate cancer development and progression.
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Copy and paste a formatted citation
Spandidos Publications style
Wu R, Wang H, Wang J, Wang P, Huang F, Xie B, Zhao Y, Li S and Zhou J: EphA3, induced by PC-1/PrLZ, contributes to the malignant progression of prostate cancer. Oncol Rep 32: 2657-2665, 2014.
APA
Wu, R., Wang, H., Wang, J., Wang, P., Huang, F., Xie, B. ... Zhou, J. (2014). EphA3, induced by PC-1/PrLZ, contributes to the malignant progression of prostate cancer. Oncology Reports, 32, 2657-2665. https://doi.org/10.3892/or.2014.3482
MLA
Wu, R., Wang, H., Wang, J., Wang, P., Huang, F., Xie, B., Zhao, Y., Li, S., Zhou, J."EphA3, induced by PC-1/PrLZ, contributes to the malignant progression of prostate cancer". Oncology Reports 32.6 (2014): 2657-2665.
Chicago
Wu, R., Wang, H., Wang, J., Wang, P., Huang, F., Xie, B., Zhao, Y., Li, S., Zhou, J."EphA3, induced by PC-1/PrLZ, contributes to the malignant progression of prostate cancer". Oncology Reports 32, no. 6 (2014): 2657-2665. https://doi.org/10.3892/or.2014.3482
Copy and paste a formatted citation
x
Spandidos Publications style
Wu R, Wang H, Wang J, Wang P, Huang F, Xie B, Zhao Y, Li S and Zhou J: EphA3, induced by PC-1/PrLZ, contributes to the malignant progression of prostate cancer. Oncol Rep 32: 2657-2665, 2014.
APA
Wu, R., Wang, H., Wang, J., Wang, P., Huang, F., Xie, B. ... Zhou, J. (2014). EphA3, induced by PC-1/PrLZ, contributes to the malignant progression of prostate cancer. Oncology Reports, 32, 2657-2665. https://doi.org/10.3892/or.2014.3482
MLA
Wu, R., Wang, H., Wang, J., Wang, P., Huang, F., Xie, B., Zhao, Y., Li, S., Zhou, J."EphA3, induced by PC-1/PrLZ, contributes to the malignant progression of prostate cancer". Oncology Reports 32.6 (2014): 2657-2665.
Chicago
Wu, R., Wang, H., Wang, J., Wang, P., Huang, F., Xie, B., Zhao, Y., Li, S., Zhou, J."EphA3, induced by PC-1/PrLZ, contributes to the malignant progression of prostate cancer". Oncology Reports 32, no. 6 (2014): 2657-2665. https://doi.org/10.3892/or.2014.3482
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