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Article

A new version of targeted minicircle producer system for EBV-positive human nasopharyngeal carcinoma

  • Authors:
    • Yufang Zuo
    • Sihai Liao
    • Zumin Xu
    • Jierong Xie
    • Wenlin Huang
    • Zhonghua Yu
  • View Affiliations / Copyright

    Affiliations: Cancer Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, P.R. China, State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, Guangzhou, Guangdong, P.R. China
  • Pages: 2564-2570
    |
    Published online on: September 17, 2014
       https://doi.org/10.3892/or.2014.3486
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Abstract

Targeted gene therapy needs to be implemented for future therapies to ensure efficient activity at the site of patient primary tumors or metastases without causing intolerable side-effects. One of the elements of gene therapy is vector, which includes viral and non-viral vector. In the present study, we constructed a novel non-viral targeted gene therapeutic system by using the new minicircle (MC) producing plasmid for Epstein-Barr virus (EBV)-positive nasopharyngeal carcinoma (NPC). Molecular cloning technique was used to construct plasmids and electrophoretic analysis. Dual-luciferase reporter assay was used to evaluate the expression of luciferase. Fluorescence microscope was used to detect the expression of enhanced green fluorescence protein (EGFP). We constructed a new MC producing system pMC.BESPX-origin of plasmid replication (oriP), and demonstrated that this system could produce highly purified MC-oriP. Furthermore, our results showed that MC-oriP vector produced by the new system could mediate targeted luciferase gene expression in EBV-positive NPC cells. In addition, we verified that MC could mediate enhanced transgene expression compared with parent plasmid through EGFP transfection. The present study constructed a targeted expression vector pMC.BESPX-oriP which could carry diversified therapeutic genes for EBV-positive NPC and provides a new approach for MC-based therapies.
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1 

Lyon AR, Sato M, Hajjar RJ, Samulski RJ and Harding SE: Gene therapy: targeting the myocardium. Heart. 94:89–99. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Marshall E: Gene therapy death prompts review of adenovirus vector. Science. 286:2244–2245. 1999. View Article : Google Scholar : PubMed/NCBI

3 

Faurez F, Dory D, Le Moigne V, Gravier R and Jestin A: Biosafety of DNA vaccines: new generation of DNA vectors and current knowledge on the fate of plasmids after injection. Vaccine. 28:3888–3895. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Ginn SL, Alexander IE, Edelstein ML, Abedi MR and Wixon J: Gene therapy clinical trials worldwide to 2012 - an update. J Gene Med. 15:65–77. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Jechlinger W: Optimization and delivery of plasmid DNA for vaccination. Expert Rev Vaccines. 5:803–825. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Huang M, Chen Z, Hu S, et al: Novel minicircle vector for gene therapy in murine myocardial infarction. Circulation. 120(Suppl 11): S230–S237. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Chen ZY, He CY, Ehrhardt A and Kay MA: Minicircle DNA vectors devoid of bacterial DNA result in persistent and high-level transgene expression in vivo. Mol Ther. 8:495–500. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Chen ZY, He CY, Meuse L and Kay MA: Silencing of episomal transgene expression by plasmid bacterial DNA elements in vivo. Gene Ther. 11:856–864. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Chen ZY, He CY and Kay MA: Improved production and purification of minicircle DNA vector free of plasmid bacterial sequences and capable of persistent transgene expression in vivo. Hum Gene Ther. 16:126–131. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Jia F, Wilson KD, Sun N, et al: A nonviral minicircle vector for deriving human iPS cells. Nat Methods. 7:197–199. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Zhang C, Gao S, Jiang W, et al: Targeted minicircle DNA delivery using folate-poly(ethylene glycol)-polyethylenimine as non-viral carrier. Biomaterials. 31:6075–6086. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Dietz WM, Skinner NE, Hamilton SE, et al: Minicircle DNA is superior to plasmid DNA in eliciting antigen-specific CD8+ T-cell responses. Mol Ther. 21:1526–1535. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Hine CM, Seluanov A and Gorbunova V: Use of the Rad51 promoter for targeted anti-cancer therapy. Proc Natl Acad Sci USA. 105:20810–20815. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Cohen JI: Epstein-Barr virus infection. N Engl J Med. 343:481–492. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Bochkarev A, Barwell JA, Pfuetzner RA, Bochkareva E, Frappier L and Edwards AM: Crystal structure of the DNA-binding domain of the Epstein-Barr virus origin-binding protein, EBNA1, bound to DNA. Cell. 84:791–800. 1996. View Article : Google Scholar : PubMed/NCBI

16 

Niedobitek G, Agathanggelou A and Nicholls JM: Epstein-Barr virus infection and the pathogenesis of nasopharyngeal carcinoma: viral gene expression, tumour cell phenotype, and the role of the lymphoid stroma. Semin Cancer Biol. 7:165–174. 1996. View Article : Google Scholar : PubMed/NCBI

17 

Altmann M, Pich D, Ruiss R, Wang J, Sugden B and Hammerschmidt W: Transcriptional activation by EBV nuclear antigen 1 is essential for the expression of EBV’s transforming genes. Proc Natl Acad Sci USA. 103:14188–14193. 2006.

18 

Leight ER and Sugden B: EBNA-1: a protein pivotal to latent infection by Epstein-Barr virus. Rev Med Virol. 10:83–100. 2000. View Article : Google Scholar : PubMed/NCBI

19 

Lindner SE and Sugden B: The plasmid replicon of Epstein-Barr virus: mechanistic insights into efficient, licensed, extrachromosomal replication in human cells. Plasmid. 58:1–12. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Li JH, Chia M, Shi W, et al: Tumor-targeted gene therapy for nasopharyngeal carcinoma. Cancer Res. 62:171–178. 2002.PubMed/NCBI

21 

Zuo Y, Wu J, Xu Z, et al: Minicircle-oriP-IFNγ: a novel targeted gene therapeutic system for EBV positive human nasopharyngeal carcinoma. PLoS One. 6:e194072011.

22 

Kay MA, He CY and Chen ZY: A robust system for production of minicircle DNA vectors. Nat Biotechnol. 28:1287–1289. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Teng ZP, Ooka T, Huang DP and Zeng Y: Detection of Epstein-Barr virus DNA in well and poorly differentiated nasopharyngeal carcinoma cell lines. Virus Genes. 13:53–60. 1996. View Article : Google Scholar : PubMed/NCBI

24 

Liu T, Ding Y, Xie W, et al: An imageable metastatic treatment model of nasopharyngeal carcinoma. Clin Cancer Res. 13:3960–3967. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Cheung ST, Huang DP, Hui AB, et al: Nasopharyngeal carcinoma cell line (C666-1) consistently harbouring Epstein-Barr virus. Int J Cancer. 83:121–126. 1999. View Article : Google Scholar : PubMed/NCBI

26 

Dong Y, Aied A, Li J, Wang Q, Hu X and Wang W: An in vitro approach for production of non-scar minicircle DNA vectors. J Biotechnol. 166:84–87. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Manjila SB, Baby JN, Bijin EN, Constantine I, Pramod K and Valsalakumari J: Novel gene delivery systems. Int J Pharm Investig. 3:1–7. 2013. View Article : Google Scholar

28 

Gao L, Xie L, Long X, et al: Efficacy of MRI visible iron oxide nanoparticles in delivering minicircle DNA into liver via intra-biliary infusion. Biomaterials. 34:3688–3696. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zuo Y, Liao S, Xu Z, Xie J, Huang W and Yu Z: A new version of targeted minicircle producer system for EBV-positive human nasopharyngeal carcinoma. Oncol Rep 32: 2564-2570, 2014.
APA
Zuo, Y., Liao, S., Xu, Z., Xie, J., Huang, W., & Yu, Z. (2014). A new version of targeted minicircle producer system for EBV-positive human nasopharyngeal carcinoma. Oncology Reports, 32, 2564-2570. https://doi.org/10.3892/or.2014.3486
MLA
Zuo, Y., Liao, S., Xu, Z., Xie, J., Huang, W., Yu, Z."A new version of targeted minicircle producer system for EBV-positive human nasopharyngeal carcinoma". Oncology Reports 32.6 (2014): 2564-2570.
Chicago
Zuo, Y., Liao, S., Xu, Z., Xie, J., Huang, W., Yu, Z."A new version of targeted minicircle producer system for EBV-positive human nasopharyngeal carcinoma". Oncology Reports 32, no. 6 (2014): 2564-2570. https://doi.org/10.3892/or.2014.3486
Copy and paste a formatted citation
x
Spandidos Publications style
Zuo Y, Liao S, Xu Z, Xie J, Huang W and Yu Z: A new version of targeted minicircle producer system for EBV-positive human nasopharyngeal carcinoma. Oncol Rep 32: 2564-2570, 2014.
APA
Zuo, Y., Liao, S., Xu, Z., Xie, J., Huang, W., & Yu, Z. (2014). A new version of targeted minicircle producer system for EBV-positive human nasopharyngeal carcinoma. Oncology Reports, 32, 2564-2570. https://doi.org/10.3892/or.2014.3486
MLA
Zuo, Y., Liao, S., Xu, Z., Xie, J., Huang, W., Yu, Z."A new version of targeted minicircle producer system for EBV-positive human nasopharyngeal carcinoma". Oncology Reports 32.6 (2014): 2564-2570.
Chicago
Zuo, Y., Liao, S., Xu, Z., Xie, J., Huang, W., Yu, Z."A new version of targeted minicircle producer system for EBV-positive human nasopharyngeal carcinoma". Oncology Reports 32, no. 6 (2014): 2564-2570. https://doi.org/10.3892/or.2014.3486
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