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Article

Overexpression of syntenin enhances hepatoma cell proliferation and invasion: Potential roles in human hepatoma

  • Authors:
    • Xuemin Liu
    • Xufeng Zhang
    • Yi Lv
    • Junxi Xiang
    • Jianhua Shi
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
  • Pages: 2810-2816
    |
    Published online on: September 18, 2014
       https://doi.org/10.3892/or.2014.3498
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Abstract

Hepatocellular carcinoma (HCC) ranks as the third leading cause of tumor-related mortality worldwide. Recently, syntenin was found to be upregulated in several tumors and to exert pivotal roles in the development of cancer. However, its function and the underlying mechanism in HCC remain to be defined. In the present study, the elevated expression levels of syntenin mRNA and protein were detected in four HCC cell lines. Overexpression of syntenin in hepatoma HCCLM3 cells enhanced cell proliferation. Furthermore, syntenin upregulation increased epidermal growth factor receptor (EGFR) expression, which accounted for syntenin‑induced cell proliferation as precondition with EGFR siRNA clearly attenuated cell proliferation in syntenin-transfected cells. At the same time, syntenin overexpression promoted cell invasion by MMP-2, as pretreatment with anti-MMP-2 antibody blocked syntenin-induced invading cell numbers. Additionally, syntenin upregulation induced the phosphorylation of p38 MAPK contributing to the increase in MMP-2 expression, as treatment with the specific inhibitor for p38 MAPK (SB203580) clearly abrogated MMP-2 expression induced by syntenin. Collectively, our results suggest that syntenin overexpression plays a critical role in promoting the proliferation and invasion of hepatoma cells. Therefore, the present study provides new insight into how syntenin accelerates the development and progression of hepatoma, and suggests that syntenin may be a promising therapeutic agent against hepatoma.
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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Zhang X, Lv Y, Xiang J and Shi J: Overexpression of syntenin enhances hepatoma cell proliferation and invasion: Potential roles in human hepatoma. Oncol Rep 32: 2810-2816, 2014.
APA
Liu, X., Zhang, X., Lv, Y., Xiang, J., & Shi, J. (2014). Overexpression of syntenin enhances hepatoma cell proliferation and invasion: Potential roles in human hepatoma. Oncology Reports, 32, 2810-2816. https://doi.org/10.3892/or.2014.3498
MLA
Liu, X., Zhang, X., Lv, Y., Xiang, J., Shi, J."Overexpression of syntenin enhances hepatoma cell proliferation and invasion: Potential roles in human hepatoma". Oncology Reports 32.6 (2014): 2810-2816.
Chicago
Liu, X., Zhang, X., Lv, Y., Xiang, J., Shi, J."Overexpression of syntenin enhances hepatoma cell proliferation and invasion: Potential roles in human hepatoma". Oncology Reports 32, no. 6 (2014): 2810-2816. https://doi.org/10.3892/or.2014.3498
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Zhang X, Lv Y, Xiang J and Shi J: Overexpression of syntenin enhances hepatoma cell proliferation and invasion: Potential roles in human hepatoma. Oncol Rep 32: 2810-2816, 2014.
APA
Liu, X., Zhang, X., Lv, Y., Xiang, J., & Shi, J. (2014). Overexpression of syntenin enhances hepatoma cell proliferation and invasion: Potential roles in human hepatoma. Oncology Reports, 32, 2810-2816. https://doi.org/10.3892/or.2014.3498
MLA
Liu, X., Zhang, X., Lv, Y., Xiang, J., Shi, J."Overexpression of syntenin enhances hepatoma cell proliferation and invasion: Potential roles in human hepatoma". Oncology Reports 32.6 (2014): 2810-2816.
Chicago
Liu, X., Zhang, X., Lv, Y., Xiang, J., Shi, J."Overexpression of syntenin enhances hepatoma cell proliferation and invasion: Potential roles in human hepatoma". Oncology Reports 32, no. 6 (2014): 2810-2816. https://doi.org/10.3892/or.2014.3498
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