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Article

Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells

  • Authors:
    • Xiuyuan Ou
    • Yan Chen
    • Xinxin Cheng
    • Xumeng Zhang
    • Qiyang He
  • View Affiliations / Copyright

    Affiliations: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, P.R. China, Academy of the State Administration of Grain, Beijing 100037, P.R. China, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, P.R. China
  • Pages: 2803-2809
    |
    Published online on: September 23, 2014
       https://doi.org/10.3892/or.2014.3512
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Abstract

Resveratrol, a natural polyphenolic phytochemical, has received considerable attention due to its potential chemopreventive and chemotherapeutic properties. In the present study, we first evaluated the growth-inhibitory effect of resveratrol on HepG2 cells and explored the underlying molecular mechanisms. Resveratrol inhibited proliferation and induced apoptosis in HepG2 cells via activation of caspase-9 and caspase-3, upregulation of the Bax/Bcl-2 ratio and induction of p53 expression. Cell cycle analysis demonstrated that resveratrol arrested cell cycle progression in the G1 and S phase. We further focused on the combination of matrine, a natural component extracted from the traditional Chinese medical herb Sophora flavescens Ait., as a mechanism to potentiate the growth-inhibitory effect of resveratrol on HepG2 cells. Both MTT and colony formation assay results indicated that the combined treatment of resveratrol and matrine exhibited a synergistic antiproliferative effect. In addition, resveratrol-induced apoptosis was significantly enhanced by matrine, which could be attributed to activation of caspase-3 and caspase-9, downregulation of survivin, induction of reactive oxygen species (ROS) generation and disruption of mitochondria membrane potential (Δψm). Our findings suggest that the combination treatment of resveratrol and matrine is a promising novel anticancer strategy for liver cancer; it also provides new insights into the mechanisms of combined therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Ou X, Chen Y, Cheng X, Zhang X and He Q: Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells. Oncol Rep 32: 2803-2809, 2014.
APA
Ou, X., Chen, Y., Cheng, X., Zhang, X., & He, Q. (2014). Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells. Oncology Reports, 32, 2803-2809. https://doi.org/10.3892/or.2014.3512
MLA
Ou, X., Chen, Y., Cheng, X., Zhang, X., He, Q."Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells". Oncology Reports 32.6 (2014): 2803-2809.
Chicago
Ou, X., Chen, Y., Cheng, X., Zhang, X., He, Q."Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells". Oncology Reports 32, no. 6 (2014): 2803-2809. https://doi.org/10.3892/or.2014.3512
Copy and paste a formatted citation
x
Spandidos Publications style
Ou X, Chen Y, Cheng X, Zhang X and He Q: Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells. Oncol Rep 32: 2803-2809, 2014.
APA
Ou, X., Chen, Y., Cheng, X., Zhang, X., & He, Q. (2014). Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells. Oncology Reports, 32, 2803-2809. https://doi.org/10.3892/or.2014.3512
MLA
Ou, X., Chen, Y., Cheng, X., Zhang, X., He, Q."Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells". Oncology Reports 32.6 (2014): 2803-2809.
Chicago
Ou, X., Chen, Y., Cheng, X., Zhang, X., He, Q."Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells". Oncology Reports 32, no. 6 (2014): 2803-2809. https://doi.org/10.3892/or.2014.3512
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