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Article

Mechanism and biological significance of the overexpression of IFITM3 in gastric cancer

  • Authors:
    • Jun Hu
    • Shuwei Wang
    • Yan Zhao
    • Qinhao  Guo
    • Dongsheng  Zhang
    • Jiejing Chen
    • Juan  Li
    • Qiang Fei
    • Yueming Sun
  • View Affiliations / Copyright

    Affiliations: Department of Colorectal Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
  • Pages: 2648-2656
    |
    Published online on: October 1, 2014
       https://doi.org/10.3892/or.2014.3522
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Abstract

Interferon‑induced transmembrane protein 3 (IFITM3) has been recently identified as a potential molecular marker. IFITM3 has been reported to be upregulated in various human diseases, including colon and breast cancer, astrocytoma, as well as ulcerative colitis. However, the clinical significance and underlying mechanisms of IFITM3 dysregulated expression in gastric cancer (GC) remain to be determined. The present study aimed to evaluate the expression of IFITM3 in human gastric tumor cells and specimens and investigate the effects of IFITM3 knockdown in the regulation of GC growth and its potential mechanism. IFITM3 expression was significantly overexpressed in the GC cell lines and GC tissues compared with corresponding normal controls by RT‑qPCR, western blot analysis and immunohistochemistry, and this overexpression was correlated with tumor differentiation, lymph node and distant metastasis, and advanced tumor node metastasis stages. Furthermore, knockdown of IFITM3 expression suppressed tumor cell migration, invasion and proliferation significantly in vitro, arrested tumor cells at the G0/G1 phase and reduced the cell numbers in the S phase of the cell cycle. We preliminarily confirmed that IFITM3 can be mediated by the activities of Wnt/β-catenin signaling. Further investigation revealed that silencing of IFITM3 effectively reversed the epithelial-to-mesenchymal transition (EMT) phenotype and reduced the activities of MMP-2 and MMP-9 expression. Taken together, these data suggested that IFITM3 is a potential therapeutic target for GC.
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Copy and paste a formatted citation
Spandidos Publications style
Hu J, Wang S, Zhao Y, Guo Q, Zhang D, Chen J, Li J, Fei Q and Sun Y: Mechanism and biological significance of the overexpression of IFITM3 in gastric cancer. Oncol Rep 32: 2648-2656, 2014.
APA
Hu, J., Wang, S., Zhao, Y., Guo, Q., Zhang, D., Chen, J. ... Sun, Y. (2014). Mechanism and biological significance of the overexpression of IFITM3 in gastric cancer. Oncology Reports, 32, 2648-2656. https://doi.org/10.3892/or.2014.3522
MLA
Hu, J., Wang, S., Zhao, Y., Guo, Q., Zhang, D., Chen, J., Li, J., Fei, Q., Sun, Y."Mechanism and biological significance of the overexpression of IFITM3 in gastric cancer". Oncology Reports 32.6 (2014): 2648-2656.
Chicago
Hu, J., Wang, S., Zhao, Y., Guo, Q., Zhang, D., Chen, J., Li, J., Fei, Q., Sun, Y."Mechanism and biological significance of the overexpression of IFITM3 in gastric cancer". Oncology Reports 32, no. 6 (2014): 2648-2656. https://doi.org/10.3892/or.2014.3522
Copy and paste a formatted citation
x
Spandidos Publications style
Hu J, Wang S, Zhao Y, Guo Q, Zhang D, Chen J, Li J, Fei Q and Sun Y: Mechanism and biological significance of the overexpression of IFITM3 in gastric cancer. Oncol Rep 32: 2648-2656, 2014.
APA
Hu, J., Wang, S., Zhao, Y., Guo, Q., Zhang, D., Chen, J. ... Sun, Y. (2014). Mechanism and biological significance of the overexpression of IFITM3 in gastric cancer. Oncology Reports, 32, 2648-2656. https://doi.org/10.3892/or.2014.3522
MLA
Hu, J., Wang, S., Zhao, Y., Guo, Q., Zhang, D., Chen, J., Li, J., Fei, Q., Sun, Y."Mechanism and biological significance of the overexpression of IFITM3 in gastric cancer". Oncology Reports 32.6 (2014): 2648-2656.
Chicago
Hu, J., Wang, S., Zhao, Y., Guo, Q., Zhang, D., Chen, J., Li, J., Fei, Q., Sun, Y."Mechanism and biological significance of the overexpression of IFITM3 in gastric cancer". Oncology Reports 32, no. 6 (2014): 2648-2656. https://doi.org/10.3892/or.2014.3522
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