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Article

Loss of ALCAM expression is linked to adverse phenotype and poor prognosis in breast cancer: A TMA-based immunohistochemical study on 2,197 breast cancer patients

  • Authors:
    • Eike Burandt
    • Tanaz Bari Noubar
    • Annette Lebeau
    • Sarah  Minner
    • Christoph Burdelski
    • Fritz  Jänicke
    • Vollkmar  Müller
    • Luigi  Terracciano
    • Ronald  Simon
    • Guido Sauter
    • Waldemar  Wilczak
    • Patrick Lebok
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany, Department of Gynecology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany, Department of Pathology, University Hospital, Basel, Switzerland
  • Pages: 2628-2634
    |
    Published online on: October 1, 2014
       https://doi.org/10.3892/or.2014.3523
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Abstract

Activated leukocyte cell adhesion molecule (ALCAM) is a membranous cell adhesion protein that is often expressed in breast cancer. Data on the prognostic impact of ALCAM expression is highly controversial in this cancer. To evaluate the clinical impact of ALCAM expression in a sufficiently large patient cohort, we utilized a tissue microarray (TMA) containing more than 2,100 primary breast cancers with clinical follow-up data by immunohistochemistry. TMA spots containing normal breast epithelium showed moderate to strong membranous ALCAM staining. ALCAM staining was strong in 66.2%, moderate in 10.9%, weak in 11.1% and absent in 11.8% of 1,778 (80.9%) interpretable breast cancer tissue spots. Decreased ALCAM expression was significantly associated with advanced tumor size (p=0.0017), unfavorable tumor grade (p<0.0001), negative ER and PR status (p<0.0001 each) as well as high Ki67 labeling index (p<0.0001). Cancers with ACLAM expression loss had a significantly poorer overall (p<0.0001) and disease-specific survival (p=0.0088). This association also held true in the subset of nodal positive cancers (p<0.0001). In conclusion, these data demonstrate that ALCAM is generally expressed in normal and cancerous breast epithelium and that a marked reduction of ALCAM expression characterizes a subset of breast cancer patients with adverse tumor characteristics and unfavorable clinical outcome.
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1 

Ohneda O, Ohneda K, Arai F, et al: ALCAM (CD166): its role in hematopoietic and endothelial development. Blood. 98:2134–2142. 2001. View Article : Google Scholar : PubMed/NCBI

2 

Masedunskas A, King JA, Tan F, et al: Activated leukocyte cell adhesion molecule is a component of the endothelial junction involved in transendothelial monocyte migration. FEBS Lett. 580:2637–2645. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Swart GW: Activated leukocyte cell adhesion molecule (CD166/ ALCAM): developmental and mechanistic aspects of cell clustering and cell migration. Eur J Cell Biol. 81:313–321. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Degen WG, van Kempen LC, Gijzen EG, et al: MEMD, a new cell adhesion molecule in metastasizing human melanoma cell lines, is identical to ALCAM (activated leukocyte cell adhesion molecule). Am J Pathol. 152:805–813. 1998.PubMed/NCBI

5 

van Kempen LC, van den Oord JJ, van Muijen GN, Weidle UH, Bloemers HP and Swart GW: Activated leukocyte cell adhesion molecule/CD166, a marker of tumor progression in primary malignant melanoma of the skin. Am J Pathol. 156:769–774. 2000.PubMed/NCBI

6 

Tomita K, van Bokhoven A, Jansen C, et al: Activated leukocyte cell adhesion molecule (ALCAM) expression is associated with a poor prognosis for bladder cancer patients. UroOncology. 3:121–129. 2003. View Article : Google Scholar

7 

Weichert W, Knösel T, Bellach J, Dietel M and Kristiansen G: ALCAM/CD166 is overexpressed in colorectal carcinoma and correlates with shortened patient survival. J Clin Pathol. 57:1160–1164. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Verma A, Shukla NK, Deo SV, Gupta SD and Ralhan R: MEMD/ ALCAM: a potential marker for tumor invasion and nodal metastasis in esophageal squamous cell carcinoma. Oncology. 68:462–470. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Kahlert C, Weber H, Mogler C, et al: Increased expression of ALCAM/CD166 in pancreatic cancer is an independent prognostic marker for poor survival and early tumour relapse. Br J Cancer. 101:457–464. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Sawhney M, Matta A, Macha MA, et al: Cytoplasmic accumulation of activated leukocyte cell adhesion molecule is a predictor of disease progression and reduced survival in oral cancer patients. Int J Cancer. 124:2098–2105. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Mezzanzanica D, Fabbi M, Bagnoli M, et al: Subcellular localization of activated leukocyte cell adhesion molecule is a molecular predictor of survival in ovarian carcinoma patients. Clin Cancer Res. 14:1726–1733. 2008. View Article : Google Scholar

12 

Corrias MV, Gambini C, Gregorio A, et al: Different subcellular localization of ALCAM molecules in neuroblastoma: association with relapse. Cell Oncol. 32:77–86. 2010.PubMed/NCBI

13 

Tomita K, van Bokhoven A, Jansen CF, Bussemakers MJ and Schalken JA: Coordinate recruitment of E-cadherin and ALCAM to cell-cell contacts by α-catenin. Biochem Biophys Res Commun. 267:870–874. 2000.PubMed/NCBI

14 

King JA, Ofori-Acquah SF, Stevens T, Al-Mehdi AB, Fodstad O and Jiang WG: Activated leukocyte cell adhesion molecule in breast cancer: prognostic indicator. Breast Cancer Res. 6:R478–R487. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Burkhardt M, Mayordomo E, Winzer KJ, et al: Cytoplasmic overexpression of ALCAM is prognostic of disease progression in breast cancer. J Clin Pathol. 59:403–409. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Wiiger MT, Gehrken HB, Fodstad Ø, Maelandsmo GM and Andersson Y: A novel human recombinant single-chain antibody targeting CD166/ALCAM inhibits cancer cell invasion in vitro and in vivo tumour growth. Cancer Immunol Immunother. 59:1665–1674. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Hein S, Muller V, Kohler N, et al: Biologic role of activated leukocyte cell adhesion molecule overexpression in breast cancer cell lines and clinical tumor tissue. Breast Cancer Res Treat. 129:347–360. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Piao D, Jiang T, Liu G, Wang B, Xu J and Zhu A: Clinical implications of activated leukocyte cell adhesion molecule expression in breast cancer. Mol Biol Rep. 39:661–668. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Davies S and Jiang WG: ALCAM, activated leukocyte cell adhesion molecule, influences the aggressive nature of breast cancer cells, a potential connection to bone metastasis. Anti-cancer Res. 30:1163–1168. 2010.

20 

Milde-Langosch K, Janke S, Wagner I, et al: Role of Fra-2 in breast cancer: influence on tumor cell invasion and motility. Breast Cancer Res Treat. 107:337–347. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Jezierska A, Matysiak W and Motyl T: ALCAM/CD166 protects breast cancer cells against apoptosis and autophagy. Med Sci Monit. 12:BR263–BR273. 2006.PubMed/NCBI

22 

Ruiz C, Seibt S, Al Kuraya K, et al: Tissue microarrays for comparing molecular features with proliferation activity in breast cancer. Int J Cancer. 118:2190–2194. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Elston CW and Ellis IO: Pathological prognostic factors in breast cancer. I The value of histological grade in breast cancer: experience from a large study with long-term follow-up. Histopathology. 19:403–410. 1991. View Article : Google Scholar

24 

McShane LM, Altman DG, Sauerbrei W, Taube SE, Gion M and Clark GM; Statistics Subcommittee of NCI-EORTC Working Group on Cancer Diagnostics. REporting recommendations for tumor MARKer prognostic studies (REMARK). Breast Cancer Res Treat. 100:229–235. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Al-Kuraya K, Schraml P, Torhorst J, et al: Prognostic relevance of gene amplifications and coamplifications in breast cancer. Cancer Res. 64:8534–8540. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Kilic E, Milde-Langosch K, Müller V, Wirtz R and Ihnen M: Expression of activated leukocyte cell adhesion molecule in breast cancer. Predictability of the response to taxane-free chemotherapy. Pathologe. 29(Suppl 2): S347–S352. 2008.(In German).

27 

Ihnen M, Müller V, Wirtz RM, et al: Predictive impact of activated leukocyte cell adhesion molecule (ALCAM/CD166) in breast cancer. Breast Cancer Res Treat. 112:419–427. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Ihnen M, Köhler N, Kersten JF, et al: Expression levels of activated leukocyte cell adhesion molecule (ALCAM/CD166) in primary breast carcinoma and distant breast cancer metastases. Dis Markers. 28:71–78. 2010. View Article : Google Scholar : PubMed/NCBI

29 

King JA, Tan F, Mbeunkui F, et al: Mechanisms of transcriptional regulation and prognostic significance of activated leukocyte cell adhesion molecule in cancer. Mol Cancer. 9:2662010. View Article : Google Scholar : PubMed/NCBI

30 

Paik S, Shak S, Tang G, et al: A multigene assay to predict recurrence of tamoxifen-treated, node-negative breast cancer. N Engl J Med. 351:2817–2826. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Glas AM, Floore A, Delahaye LJ, et al: Converting a breast cancer microarray signature into a high-throughput diagnostic test. BMC Genomics. 7:2782006. View Article : Google Scholar : PubMed/NCBI

32 

Paik S, Tang G, Shak S, et al: Gene expression and benefit of chemotherapy in women with node-negative, estrogen receptor-positive breast cancer. J Clin Oncol. 24:3726–3734. 2006. View Article : Google Scholar : PubMed/NCBI

33 

Sparano JA and Paik S: Development of the 21-gene assay and its application in clinical practice and clinical trials. J Clin Oncol. 26:721–728. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Carlson JJ and Roth JA: The impact of the Oncotype Dx breast cancer assay in clinical practice: a systematic review and meta-analysis. Breast Cancer Res Treat. 141:13–22. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Roth A, Drummond DC, Conrad F, et al: Anti-CD166 single chain antibody-mediated intracellular delivery of liposomal drugs to prostate cancer cells. Mol Cancer Ther. 6:2737–2746. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Burandt E, Bari Noubar T, Lebeau A, Minner S, Burdelski C, Jänicke F, Müller V, Terracciano L, Simon R, Sauter G, Sauter G, et al: Loss of ALCAM expression is linked to adverse phenotype and poor prognosis in breast cancer: A TMA-based immunohistochemical study on 2,197 breast cancer patients. Oncol Rep 32: 2628-2634, 2014.
APA
Burandt, E., Bari Noubar, T., Lebeau, A., Minner, S., Burdelski, C., Jänicke, F. ... Lebok, P. (2014). Loss of ALCAM expression is linked to adverse phenotype and poor prognosis in breast cancer: A TMA-based immunohistochemical study on 2,197 breast cancer patients. Oncology Reports, 32, 2628-2634. https://doi.org/10.3892/or.2014.3523
MLA
Burandt, E., Bari Noubar, T., Lebeau, A., Minner, S., Burdelski, C., Jänicke, F., Müller, V., Terracciano, L., Simon, R., Sauter, G., Wilczak, W., Lebok, P."Loss of ALCAM expression is linked to adverse phenotype and poor prognosis in breast cancer: A TMA-based immunohistochemical study on 2,197 breast cancer patients". Oncology Reports 32.6 (2014): 2628-2634.
Chicago
Burandt, E., Bari Noubar, T., Lebeau, A., Minner, S., Burdelski, C., Jänicke, F., Müller, V., Terracciano, L., Simon, R., Sauter, G., Wilczak, W., Lebok, P."Loss of ALCAM expression is linked to adverse phenotype and poor prognosis in breast cancer: A TMA-based immunohistochemical study on 2,197 breast cancer patients". Oncology Reports 32, no. 6 (2014): 2628-2634. https://doi.org/10.3892/or.2014.3523
Copy and paste a formatted citation
x
Spandidos Publications style
Burandt E, Bari Noubar T, Lebeau A, Minner S, Burdelski C, Jänicke F, Müller V, Terracciano L, Simon R, Sauter G, Sauter G, et al: Loss of ALCAM expression is linked to adverse phenotype and poor prognosis in breast cancer: A TMA-based immunohistochemical study on 2,197 breast cancer patients. Oncol Rep 32: 2628-2634, 2014.
APA
Burandt, E., Bari Noubar, T., Lebeau, A., Minner, S., Burdelski, C., Jänicke, F. ... Lebok, P. (2014). Loss of ALCAM expression is linked to adverse phenotype and poor prognosis in breast cancer: A TMA-based immunohistochemical study on 2,197 breast cancer patients. Oncology Reports, 32, 2628-2634. https://doi.org/10.3892/or.2014.3523
MLA
Burandt, E., Bari Noubar, T., Lebeau, A., Minner, S., Burdelski, C., Jänicke, F., Müller, V., Terracciano, L., Simon, R., Sauter, G., Wilczak, W., Lebok, P."Loss of ALCAM expression is linked to adverse phenotype and poor prognosis in breast cancer: A TMA-based immunohistochemical study on 2,197 breast cancer patients". Oncology Reports 32.6 (2014): 2628-2634.
Chicago
Burandt, E., Bari Noubar, T., Lebeau, A., Minner, S., Burdelski, C., Jänicke, F., Müller, V., Terracciano, L., Simon, R., Sauter, G., Wilczak, W., Lebok, P."Loss of ALCAM expression is linked to adverse phenotype and poor prognosis in breast cancer: A TMA-based immunohistochemical study on 2,197 breast cancer patients". Oncology Reports 32, no. 6 (2014): 2628-2634. https://doi.org/10.3892/or.2014.3523
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