Knockout of the HCC suppressor gene Lass2 downregulates the expression level of miR-694

  • Authors:
    • Xiaodong Lu
    • Yuanyuan Chen
    • Tiantian Zeng
    • Lufang  Chen
    • Qixiang Shao
    • Wenxin Qin
  • View Affiliations

  • Published online on: October 3, 2014     https://doi.org/10.3892/or.2014.3527
  • Pages: 2696-2702
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Homo sapiens longevity assurance homolog 2 of yeast LAG (Lass2) catalyzes the synthesis of long-chain ceramide which is an essential element of membranous structures. Deletion of Lass2 is associated with a high risk of spontaneous or DEN-induced hepatocellular carcinoma (HCC), yet the mechanism remains unclear. In the present study, we found extensive vesicles in hepatocytes of one-month-old Lass2-knockout (KO) mice. Hepatic biochemical indices were increased and expression of albumin was attenuated in the one‑month Lass2-KO liver. The results indicate that the injuries of the hepatocytes in young Lass2-KO mice, based on the results of Gene Ontology analysis of mRNA microarray of Lass2-KO liver vs. wild-type liver showed ‘wounding response’ was the mostly possible altered pathway in the Lass2-KO mice. miR-mRNA integrated analysis revealed that miR-694 was downregulated while its target gene tumor necrosis factor α-induced protein 3 (Tnfaip3) was upregulated, as confirmed by qPCR. The expression of NF-κB which is negatively controlled by Tnfaip3 was detected by qPCR and was found to be downregulated. Herein, we first report that Lass2 deficiency caused the downregulation of miR-694 and the upregulation of its target gene Tnfaip3 in vivo in mice, which may be related to a high risk of occurrence of HCC.
View Figures
View References

Related Articles

Journal Cover

December-2014
Volume 32 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lu X, Chen Y, Zeng T, Chen L, Shao Q and Qin W: Knockout of the HCC suppressor gene Lass2 downregulates the expression level of miR-694. Oncol Rep 32: 2696-2702, 2014.
APA
Lu, X., Chen, Y., Zeng, T., Chen, L., Shao, Q., & Qin, W. (2014). Knockout of the HCC suppressor gene Lass2 downregulates the expression level of miR-694. Oncology Reports, 32, 2696-2702. https://doi.org/10.3892/or.2014.3527
MLA
Lu, X., Chen, Y., Zeng, T., Chen, L., Shao, Q., Qin, W."Knockout of the HCC suppressor gene Lass2 downregulates the expression level of miR-694". Oncology Reports 32.6 (2014): 2696-2702.
Chicago
Lu, X., Chen, Y., Zeng, T., Chen, L., Shao, Q., Qin, W."Knockout of the HCC suppressor gene Lass2 downregulates the expression level of miR-694". Oncology Reports 32, no. 6 (2014): 2696-2702. https://doi.org/10.3892/or.2014.3527